The new application of photosensitization reaction to atrial fibrillation treatment: Mechanism and demonstration of non-thermal electrical conduction block with porcine heart

Arisa Ito, Hiroki Matsuo, Tsukasa Suenari, Takuro Kajihara, Takehiro Kimura, Shunichiro Miyoshi, Satoshi Ogawa, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We have proposed non-thermal electrical conduction block for atrial fibrillation treatment by the photosensitization reaction, in which the interval time between the photosensitizer injection and irradiation is less than tenth of that in conventional way. To study the mechanism of photosensitization reaction-induced electrical conduction block, intracellular Ca2+ concentration change in rat myocardial cells was measured by fluorescent Ca 2+ indicator Fluo-4 AM with confocal laser microscopy. Measured rapid increase in the fluorescence intensity and a change in cell morphology indicated that cell membrane damage; that is Ca2+ influx and eventually cell death caused by the photosensitization reaction. To demonstrate myocardial electrical conduction block induced by the photosensitization reaction, surgically exposed porcine heart under deep anesthesia was used. The myocardial tissue was paced with a stimulation electrode. The propagated electrical signals were measured by bipolar electrodes at two different positions. Thirty minutes after the injection of 5-10 mg/kg Porfimer sodium or Talaporfin sodium, the red laser light was irradiated to the tissue point by point crossing the measuring positions by the total energy density of less than 200 J/cm2. The electrical signal conduction between the measuring electrodes in the myocardial tissue was delayed by each irradiation procedure. The electrical conduction delay corresponded to the block line length was obtained. These results demonstrated the possibility of non-thermal electrical conduction block for atrial fibrillation treatment by the photosensitization reaction.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7380
DOIs
Publication statusPublished - 2009
Event12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future - Seattle, WA, United States
Duration: 2009 Jun 112009 Jun 15

Other

Other12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future
CountryUnited States
CitySeattle, WA
Period09/6/1109/6/15

Fingerprint

fibrillation
Photosensitivity Disorders
Atrial Fibrillation
Demonstrations
Swine
Tissue
conduction
Electrodes
Sodium
Dihematoporphyrin Ether
Irradiation
Photosensitizing Agents
Photosensitizers
Confocal Microscopy
Lasers
Cell death
Cell membranes
electrodes
Rats
Microscopic examination

Keywords

  • electrical conduction block
  • intracellular free Ca concentration
  • photosensitization reaction
  • Porfimer sodium
  • Talaporfin sodium
  • therapy (PDT)

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Ito, A., Matsuo, H., Suenari, T., Kajihara, T., Kimura, T., Miyoshi, S., ... Arai, T. (2009). The new application of photosensitization reaction to atrial fibrillation treatment: Mechanism and demonstration of non-thermal electrical conduction block with porcine heart. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7380). [738051] https://doi.org/10.1117/12.822924

The new application of photosensitization reaction to atrial fibrillation treatment : Mechanism and demonstration of non-thermal electrical conduction block with porcine heart. / Ito, Arisa; Matsuo, Hiroki; Suenari, Tsukasa; Kajihara, Takuro; Kimura, Takehiro; Miyoshi, Shunichiro; Ogawa, Satoshi; Arai, Tsunenori.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7380 2009. 738051.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ito, A, Matsuo, H, Suenari, T, Kajihara, T, Kimura, T, Miyoshi, S, Ogawa, S & Arai, T 2009, The new application of photosensitization reaction to atrial fibrillation treatment: Mechanism and demonstration of non-thermal electrical conduction block with porcine heart. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7380, 738051, 12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future, Seattle, WA, United States, 09/6/11. https://doi.org/10.1117/12.822924
Ito A, Matsuo H, Suenari T, Kajihara T, Kimura T, Miyoshi S et al. The new application of photosensitization reaction to atrial fibrillation treatment: Mechanism and demonstration of non-thermal electrical conduction block with porcine heart. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7380. 2009. 738051 https://doi.org/10.1117/12.822924
Ito, Arisa ; Matsuo, Hiroki ; Suenari, Tsukasa ; Kajihara, Takuro ; Kimura, Takehiro ; Miyoshi, Shunichiro ; Ogawa, Satoshi ; Arai, Tsunenori. / The new application of photosensitization reaction to atrial fibrillation treatment : Mechanism and demonstration of non-thermal electrical conduction block with porcine heart. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7380 2009.
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