The NF-κB RelA subunit confers resistance to Leishmania major by inducing nitric oxide synthase 2 and fas expression but not Th1 differentiation

Setsuko Mise-Omata, Etsushi Kuroda, Tsutomu Sugiura, Uki Yamashita, Yuichi Obata, Takahiro S. Doi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Although the NF-κB transcription factors participate in both innate and adaptive immune responses, little is known about the role of the RelA subunit because mice lacking the rela gene die at embryonic day 14. To elucidate the role of RelA in Leishmania major infection, we prepared fetal liver chimeric mice by adoptively transferring embryonic day 13.5 rela-/- or rela+/+ fetal liver into lethally irradiated host mice. About 90% of the peripheral lymphocytes of the chimeric mice had differentiated from rela fetal liver cells. The rela-/- fetal liver chimeric mice were highly sensitive to infection with L. major and died within 11 wk after infection. Despite the severity of the disease, parasite Ag-reactive Th1 cells developed normally. The rela-/- macrophages were less able to control intracellular parasite replication than rela+/+ macrophages, despite showing equally efficient phagocytosis. Both in vitro NO production of macrophages and in vivo expression of NO synthase 2 in the lesions and draining lymph nodes was reduced in rela-/- fetal liver chimeric mice. Moreover, up-regulation of Fas in rela-/- macrophages was impaired both after in vitro stimulation with LPS and after in vivo infection with L. major, implying a defect in their ability to eliminate infected cells. Thus, RelA is necessary for macrophages to be resistant to intracellular parasite infection.

Original languageEnglish
Pages (from-to)4910-4916
Number of pages7
JournalJournal of Immunology
Volume182
Issue number8
DOIs
Publication statusPublished - 2009 Apr 15
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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