The NIA cDNA project in mouse stem cells and early embryos

Mark G. Carter, Yulan Piao, Dawood B. Dudekula, Yong Qian, Vincent VanBuren, Alexei A. Sharov, Tetsuya S. Tanaka, Patrick R. Martin, Uwem C. Bassey, Carole A. Stagg, Kazuhiro Aiba, Toshio Hamatani, Ryo Matoba, George J. Kargul, Minoru S.H. Ko

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

A catalog of mouse genes expressed in early embryos, embryonic and adult stem cells was assembled, including 250 000 ESTs, representing approximately 39 000 unique transcripts. The cDNA libraries, enriched in full-length clones, were condensed into the NIA 15 and 7.4K clone sets, freely distributed to the research community, providing a standard platform for expression studies using microarrays. They are essential tools for studying mammalian development and stem cell biology, and to provide hints about the differential nature of embryonic and adult stem cells.

Original languageEnglish
Pages (from-to)931-940
Number of pages10
JournalComptes Rendus - Biologies
Volume326
Issue number10-11
DOIs
Publication statusPublished - 2003 Jan 1
Externally publishedYes

Keywords

  • Embryo
  • Genomics
  • Microarray
  • Pre-implantation development
  • Stem cell

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

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