The noninvasive treatment for sentinel lymph node metastasis by photodynamic therapy using phospholipid polymer as a nanotransporter of verteporfin

Kyosuke Shimada, Sachiko Matsuda, Hiromitsu Jinno, Noriaki Kameyama, Tomohiro Konno, Tsunenori Arai, Kazuhiko Ishihara, Yuukou Kitagawa

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Abstract

Aim. The usefulness of photodynamic therapy (PDT) for treating sentinel lymph node (SLN) metastasis was evaluated. Materials and Methods. Verteporfin, a hydrophobic photosensitizer, forms a soluble aggregate with poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) (PMB). The concentrations of verteporfin were determined by measuring the fluorescence emitted at 700 nm. Seven days after the inoculation of A431 cells at the forearm of BALB/c nude mice, PMB-verteporfin was injected at dorsum manus and 75 J of light energy was delivered for 1 minute. Fifty-three mice were randomly assigned to the combination of PMB-verteporfin injection and light exposure, light exposure alone, PMB-verteporfin injection alone, and no treatment groups. Ten days after PDT, brachial lymph nodes, which were considered as SLNs, were harvested and evaluated. Results. The concentration of verteporfin in SLN was significantly higher than other organs. The combination of PMB-verteporfin injection and light exposure group significantly reduced the SLN metastasis (13%) comparing with no treatment group (52%), light exposure alone group (57%), and PMB-verteporfin injection alone group (46%). Conclusions. These data suggested that PDT using PMB as a nanotransporter of verteporfin could be a minimally invasive treatment of SLN metastasis in breast cancer and represent a potential alternative procedure to SLNB.

Original languageEnglish
Article number7412865
JournalBioMed Research International
Volume2017
DOIs
Publication statusPublished - 2017

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Photodynamic therapy
Photochemotherapy
Phospholipids
Polymers
Neoplasm Metastasis
Light
Injections
Therapeutics
verteporfin
Sentinel Lymph Node
cyhalothrin
Photosensitizing Agents
Forearm
Nude Mice
Arm
Fluorescence
Lymph Nodes
Breast Neoplasms

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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The noninvasive treatment for sentinel lymph node metastasis by photodynamic therapy using phospholipid polymer as a nanotransporter of verteporfin. / Shimada, Kyosuke; Matsuda, Sachiko; Jinno, Hiromitsu; Kameyama, Noriaki; Konno, Tomohiro; Arai, Tsunenori; Ishihara, Kazuhiko; Kitagawa, Yuukou.

In: BioMed Research International, Vol. 2017, 7412865, 2017.

Research output: Contribution to journalArticle

Shimada, Kyosuke ; Matsuda, Sachiko ; Jinno, Hiromitsu ; Kameyama, Noriaki ; Konno, Tomohiro ; Arai, Tsunenori ; Ishihara, Kazuhiko ; Kitagawa, Yuukou. / The noninvasive treatment for sentinel lymph node metastasis by photodynamic therapy using phospholipid polymer as a nanotransporter of verteporfin. In: BioMed Research International. 2017 ; Vol. 2017.
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abstract = "Aim. The usefulness of photodynamic therapy (PDT) for treating sentinel lymph node (SLN) metastasis was evaluated. Materials and Methods. Verteporfin, a hydrophobic photosensitizer, forms a soluble aggregate with poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) (PMB). The concentrations of verteporfin were determined by measuring the fluorescence emitted at 700 nm. Seven days after the inoculation of A431 cells at the forearm of BALB/c nude mice, PMB-verteporfin was injected at dorsum manus and 75 J of light energy was delivered for 1 minute. Fifty-three mice were randomly assigned to the combination of PMB-verteporfin injection and light exposure, light exposure alone, PMB-verteporfin injection alone, and no treatment groups. Ten days after PDT, brachial lymph nodes, which were considered as SLNs, were harvested and evaluated. Results. The concentration of verteporfin in SLN was significantly higher than other organs. The combination of PMB-verteporfin injection and light exposure group significantly reduced the SLN metastasis (13{\%}) comparing with no treatment group (52{\%}), light exposure alone group (57{\%}), and PMB-verteporfin injection alone group (46{\%}). Conclusions. These data suggested that PDT using PMB as a nanotransporter of verteporfin could be a minimally invasive treatment of SLN metastasis in breast cancer and represent a potential alternative procedure to SLNB.",
author = "Kyosuke Shimada and Sachiko Matsuda and Hiromitsu Jinno and Noriaki Kameyama and Tomohiro Konno and Tsunenori Arai and Kazuhiko Ishihara and Yuukou Kitagawa",
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AU - Shimada, Kyosuke

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AU - Kameyama, Noriaki

AU - Konno, Tomohiro

AU - Arai, Tsunenori

AU - Ishihara, Kazuhiko

AU - Kitagawa, Yuukou

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N2 - Aim. The usefulness of photodynamic therapy (PDT) for treating sentinel lymph node (SLN) metastasis was evaluated. Materials and Methods. Verteporfin, a hydrophobic photosensitizer, forms a soluble aggregate with poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) (PMB). The concentrations of verteporfin were determined by measuring the fluorescence emitted at 700 nm. Seven days after the inoculation of A431 cells at the forearm of BALB/c nude mice, PMB-verteporfin was injected at dorsum manus and 75 J of light energy was delivered for 1 minute. Fifty-three mice were randomly assigned to the combination of PMB-verteporfin injection and light exposure, light exposure alone, PMB-verteporfin injection alone, and no treatment groups. Ten days after PDT, brachial lymph nodes, which were considered as SLNs, were harvested and evaluated. Results. The concentration of verteporfin in SLN was significantly higher than other organs. The combination of PMB-verteporfin injection and light exposure group significantly reduced the SLN metastasis (13%) comparing with no treatment group (52%), light exposure alone group (57%), and PMB-verteporfin injection alone group (46%). Conclusions. These data suggested that PDT using PMB as a nanotransporter of verteporfin could be a minimally invasive treatment of SLN metastasis in breast cancer and represent a potential alternative procedure to SLNB.

AB - Aim. The usefulness of photodynamic therapy (PDT) for treating sentinel lymph node (SLN) metastasis was evaluated. Materials and Methods. Verteporfin, a hydrophobic photosensitizer, forms a soluble aggregate with poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) (PMB). The concentrations of verteporfin were determined by measuring the fluorescence emitted at 700 nm. Seven days after the inoculation of A431 cells at the forearm of BALB/c nude mice, PMB-verteporfin was injected at dorsum manus and 75 J of light energy was delivered for 1 minute. Fifty-three mice were randomly assigned to the combination of PMB-verteporfin injection and light exposure, light exposure alone, PMB-verteporfin injection alone, and no treatment groups. Ten days after PDT, brachial lymph nodes, which were considered as SLNs, were harvested and evaluated. Results. The concentration of verteporfin in SLN was significantly higher than other organs. The combination of PMB-verteporfin injection and light exposure group significantly reduced the SLN metastasis (13%) comparing with no treatment group (52%), light exposure alone group (57%), and PMB-verteporfin injection alone group (46%). Conclusions. These data suggested that PDT using PMB as a nanotransporter of verteporfin could be a minimally invasive treatment of SLN metastasis in breast cancer and represent a potential alternative procedure to SLNB.

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