The number of Langerhans' cells in corneal limbus decreases during preservation

N. Shinozaki, J. Shimazaki, A. Hirakata, M. Fukuda, Kazuo Tsubota

Research output: Contribution to journalArticle

Abstract

Purpose. To estimate the distribution of Langerhans' (LH) cells in limbal tissue of the cornea during preservation. Methods. The human limbal tissue was placed in storage media (Optisol GSR) for 1, 2, 3 and 5 days after the donation to eye banks. The tissue was then cut in two pieces. One piece was fixed in Dent's fixative for immunohistochemistry which was carried out with anti-S100 antibody and anti-GD1a antibody. The other piece was placed in 1.25% Glutaraldehyde overnight at 4°C for electron microscopic observation. The cells in the medium were also collected for the immunohistochemistry. Results. The number of LH cells were high in the timbal tissue which was cultured in Optisol GSR for one day. The cell distribution density decreases with culturing time. In the tissue which was cultured for five days, the LH cells were not observed. LH cells were observed in the storage medium after 3 days preservation. Conclusions. The LH cells in limbus of the cornea decreased during the preservation over three days. Although the mechanism is not known, the presence of LH cells in the media suggests the migration of the cells. It is suggested that in case of limbal transplantation of the cornea, rejection rate may decrease if the donor tissue has been preserved over three days.

Original languageEnglish
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
Publication statusPublished - 1996 Feb 15
Externally publishedYes

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Limbus Corneae
Langerhans Cells
Anti-Idiotypic Antibodies
Immunohistochemistry
Eye Banks
Fixatives
Graft Rejection
Glutaral
Cornea
Cell Movement
Cell Count
Tissue Donors
Electrons

ASJC Scopus subject areas

  • Ophthalmology

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The number of Langerhans' cells in corneal limbus decreases during preservation. / Shinozaki, N.; Shimazaki, J.; Hirakata, A.; Fukuda, M.; Tsubota, Kazuo.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

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abstract = "Purpose. To estimate the distribution of Langerhans' (LH) cells in limbal tissue of the cornea during preservation. Methods. The human limbal tissue was placed in storage media (Optisol GSR) for 1, 2, 3 and 5 days after the donation to eye banks. The tissue was then cut in two pieces. One piece was fixed in Dent's fixative for immunohistochemistry which was carried out with anti-S100 antibody and anti-GD1a antibody. The other piece was placed in 1.25{\%} Glutaraldehyde overnight at 4°C for electron microscopic observation. The cells in the medium were also collected for the immunohistochemistry. Results. The number of LH cells were high in the timbal tissue which was cultured in Optisol GSR for one day. The cell distribution density decreases with culturing time. In the tissue which was cultured for five days, the LH cells were not observed. LH cells were observed in the storage medium after 3 days preservation. Conclusions. The LH cells in limbus of the cornea decreased during the preservation over three days. Although the mechanism is not known, the presence of LH cells in the media suggests the migration of the cells. It is suggested that in case of limbal transplantation of the cornea, rejection rate may decrease if the donor tissue has been preserved over three days.",
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AU - Shimazaki, J.

AU - Hirakata, A.

AU - Fukuda, M.

AU - Tsubota, Kazuo

PY - 1996/2/15

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