The oocyte-specific linker histone H1FOO plays a key role in establishing high-quality mouse induced pluripotent stem cells

Akira Kunitomi, Keiichi Fukuda

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The faster and more efficient reprogramming done by somatic cell nuclear transfer (SCNT) than by induced pluripotent stem cell (iPSC) generation suggests that oocyte constituents have a pivotal role in promoting somatic cell reprogramming. The mammalian oocyte-specific linker histone H1FOO decondenses the chromatin of sperm nuclei after fertilization or of transferred somatic cell nuclei after SCNT in oocytes and has beneficial effects on mouse iPSC generation. The induction of H1foo with Oct4, Sox2, and Klf4 in mouse somatic cells significantly enhances the efficiency of qualified iPSC generation. Notably, H1foo promotes in vitro differentiation characteristics with low heterogeneity in iPSCs. Furthermore, H1foo enhances the generation of germline competent chimeric mice from iPSCs in a manner similar to that for embryonic stem cells. These findings indicate that H1foo contributes to the generation of higher-quality mouse iPSCs.

Original languageEnglish
Title of host publicationMolecular Players in iPSC Technology
PublisherElsevier
Pages381-401
Number of pages21
ISBN (Electronic)9780323900591
DOIs
Publication statusPublished - 2021 Jan 1
Externally publishedYes

Keywords

  • Chimeric mouse
  • Chromatin decondensation
  • Embryoid body (EB)
  • H1foo
  • Imprinted gene
  • Induced pluripotent stem cell (iPSC)
  • Linker histone
  • Oocyte
  • Reprogramming
  • Somatic cell nuclear transfer (SCNT)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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