The organization of the human immunoglobulin λ gene locus

K. Kawasaki, S. Minoshima, K. Schooler, J. Kudoh, S. Asakawa, P. J. De Jong, N. Shimizu

Research output: Contribution to journalArticle

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Abstract

To elucidate the complex structure of the human immunoglobulin λ gene locus, a 1020-kb contig was constructed using 184 cosmid clones and one bacterial artificial chromosome (BAC) clone. A high-resolution physical map of this contig revealed that the entire λ gene locus is 911 kb in length. It contains seven constant region (C(λ)) gene segments and 69 unique EcoRI-HindIII segments that hybridize to variable region gene (V(λ) probes. The VpreB gene, BCRL4, and γ-glutamyl transpeptidase gene (GGT)-like sequences are also located within the λ gene locus. Hybridization analysis suggested that the λ gene locus has undergone extensive amplification events in evolution.

Original languageEnglish
Pages (from-to)125-135
Number of pages11
JournalGenome Research
Volume5
Issue number2
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    Kawasaki, K., Minoshima, S., Schooler, K., Kudoh, J., Asakawa, S., De Jong, P. J., & Shimizu, N. (1995). The organization of the human immunoglobulin λ gene locus. Genome Research, 5(2), 125-135.