The organization of the human immunoglobulin λ gene locus

K. Kawasaki, S. Minoshima, K. Schooler, Jun Kudo, S. Asakawa, P. J. De Jong, N. Shimizu

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

To elucidate the complex structure of the human immunoglobulin λ gene locus, a 1020-kb contig was constructed using 184 cosmid clones and one bacterial artificial chromosome (BAC) clone. A high-resolution physical map of this contig revealed that the entire λ gene locus is 911 kb in length. It contains seven constant region (C(λ)) gene segments and 69 unique EcoRI-HindIII segments that hybridize to variable region gene (V(λ) probes. The VpreB gene, BCRL4, and γ-glutamyl transpeptidase gene (GGT)-like sequences are also located within the λ gene locus. Hybridization analysis suggested that the λ gene locus has undergone extensive amplification events in evolution.

Original languageEnglish
Pages (from-to)125-135
Number of pages11
JournalGenome Research
Volume5
Issue number2
Publication statusPublished - 1995

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Immunoglobulin Genes
Genes
Clone Cells
Bacterial Artificial Chromosomes
Cosmids
gamma-Glutamyltransferase

ASJC Scopus subject areas

  • Genetics

Cite this

Kawasaki, K., Minoshima, S., Schooler, K., Kudo, J., Asakawa, S., De Jong, P. J., & Shimizu, N. (1995). The organization of the human immunoglobulin λ gene locus. Genome Research, 5(2), 125-135.

The organization of the human immunoglobulin λ gene locus. / Kawasaki, K.; Minoshima, S.; Schooler, K.; Kudo, Jun; Asakawa, S.; De Jong, P. J.; Shimizu, N.

In: Genome Research, Vol. 5, No. 2, 1995, p. 125-135.

Research output: Contribution to journalArticle

Kawasaki, K, Minoshima, S, Schooler, K, Kudo, J, Asakawa, S, De Jong, PJ & Shimizu, N 1995, 'The organization of the human immunoglobulin λ gene locus', Genome Research, vol. 5, no. 2, pp. 125-135.
Kawasaki K, Minoshima S, Schooler K, Kudo J, Asakawa S, De Jong PJ et al. The organization of the human immunoglobulin λ gene locus. Genome Research. 1995;5(2):125-135.
Kawasaki, K. ; Minoshima, S. ; Schooler, K. ; Kudo, Jun ; Asakawa, S. ; De Jong, P. J. ; Shimizu, N. / The organization of the human immunoglobulin λ gene locus. In: Genome Research. 1995 ; Vol. 5, No. 2. pp. 125-135.
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