The patch-clamp and planar lipid bilayer techniques: Powerful and versatile tools to investigate the CFTR Cl- channel

David N. Sheppard, Michael A. Gray, Xiandi Gong, Yoshiro Sohma, Ilana Kogan, Dale J. Benos, Toby S. Scott-Ward, Jeng Haur Chen, Hongyu Li, Zhiwei Cai, Jyoti Gupta, Canhui Li, Mohabir Ramjeesingh, Bakhrom K. Berdiev, Iskander I. Ismailov, Christine E. Bear, Tzyh Chang Hwang, Paul Linsdell, Martin J. Hug

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism of action, interaction with other proteins and physiological role. Using the PLB technique, the structure and function of CFTR can be investigated free from the influence of other proteins. Here we discuss how these techniques are employed to investigate the CFTR Cl- channel with special emphasis on its permeation, conduction and gating properties.

Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalJournal of Cystic Fibrosis
Volume3
Issue numberSUPPL. 2
DOIs
Publication statusPublished - 2004 Aug
Externally publishedYes

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Keywords

  • Anion permeation
  • Channel gating
  • Channel regulation
  • Chloride channel activity
  • Single-channel recording
  • Whole-cell recording

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Sheppard, D. N., Gray, M. A., Gong, X., Sohma, Y., Kogan, I., Benos, D. J., Scott-Ward, T. S., Chen, J. H., Li, H., Cai, Z., Gupta, J., Li, C., Ramjeesingh, M., Berdiev, B. K., Ismailov, I. I., Bear, C. E., Hwang, T. C., Linsdell, P., & Hug, M. J. (2004). The patch-clamp and planar lipid bilayer techniques: Powerful and versatile tools to investigate the CFTR Cl- channel. Journal of Cystic Fibrosis, 3(SUPPL. 2), 101-108. https://doi.org/10.1016/j.jcf.2004.05.046