The patch-clamp and planar lipid bilayer techniques: Powerful and versatile tools to investigate the CFTR Cl- channel

David N. Sheppard; Michael A. Gray; Xiandi Gong; Yoshiro Sohma; Ilana Kogan; Dale J. Benos; Toby S. Scott-Ward; Jeng Haur Chen; Hongyu Li; Zhiwei Cai; Jyoti Gupta; Canhui Li; Mohabir Ramjeesingh; Bakhrom K. Berdiev; Iskander I. Ismailov; Christine E. Bear; Tzyh Chang Hwang; Paul Linsdell; Martin J. Hug

doi:10.1016/j.jcf.2004.05.046

The patch-clamp and planar lipid bilayer techniques: Powerful and versatile tools to investigate the CFTR Cl^- channel

David N. Sheppard, Michael A. Gray, Xiandi Gong, Yoshiro Sohma, Ilana Kogan, Dale J. Benos, Toby S. Scott-Ward, Jeng Haur Chen, Hongyu Li, Zhiwei Cai, Jyoti Gupta, Canhui Li, Mohabir Ramjeesingh, Bakhrom K. Berdiev, Iskander I. Ismailov, Christine E. Bear, Tzyh Chang Hwang, Paul Linsdell, Martin J. Hug

Department of Pharmacology

Research output: Contribution to journal › Article

20 Citations (Scopus)

Abstract

Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl^- channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism of action, interaction with other proteins and physiological role. Using the PLB technique, the structure and function of CFTR can be investigated free from the influence of other proteins. Here we discuss how these techniques are employed to investigate the CFTR Cl^- channel with special emphasis on its permeation, conduction and gating properties.

Original language	English
Pages (from-to)	101-108
Number of pages	8
Journal	Journal of Cystic Fibrosis
Volume	3
Issue number	SUPPL. 2
DOIs	https://doi.org/10.1016/j.jcf.2004.05.046
Publication status	Published - 2004 Aug

Keywords

Anion permeation
Channel gating
Channel regulation
Chloride channel activity
Single-channel recording
Whole-cell recording

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Pulmonary and Respiratory Medicine

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Sheppard, D. N., Gray, M. A., Gong, X., Sohma, Y., Kogan, I., Benos, D. J., Scott-Ward, T. S., Chen, J. H., Li, H., Cai, Z., Gupta, J., Li, C., Ramjeesingh, M., Berdiev, B. K., Ismailov, I. I., Bear, C. E., Hwang, T. C., Linsdell, P., & Hug, M. J. (2004). The patch-clamp and planar lipid bilayer techniques: Powerful and versatile tools to investigate the CFTR Cl^- channel. Journal of Cystic Fibrosis, 3(SUPPL. 2), 101-108. https://doi.org/10.1016/j.jcf.2004.05.046

The patch-clamp and planar lipid bilayer techniques : Powerful and versatile tools to investigate the CFTR Cl^- channel. / Sheppard, David N.; Gray, Michael A.; Gong, Xiandi; Sohma, Yoshiro; Kogan, Ilana; Benos, Dale J.; Scott-Ward, Toby S.; Chen, Jeng Haur; Li, Hongyu; Cai, Zhiwei; Gupta, Jyoti; Li, Canhui; Ramjeesingh, Mohabir; Berdiev, Bakhrom K.; Ismailov, Iskander I.; Bear, Christine E.; Hwang, Tzyh Chang; Linsdell, Paul; Hug, Martin J.

In: Journal of Cystic Fibrosis, Vol. 3, No. SUPPL. 2, 08.2004, p. 101-108.

Research output: Contribution to journal › Article

Sheppard, DN, Gray, MA, Gong, X, Sohma, Y, Kogan, I, Benos, DJ, Scott-Ward, TS, Chen, JH, Li, H, Cai, Z, Gupta, J, Li, C, Ramjeesingh, M, Berdiev, BK, Ismailov, II, Bear, CE, Hwang, TC, Linsdell, P & Hug, MJ 2004, 'The patch-clamp and planar lipid bilayer techniques: Powerful and versatile tools to investigate the CFTR Cl^- channel', Journal of Cystic Fibrosis, vol. 3, no. SUPPL. 2, pp. 101-108. https://doi.org/10.1016/j.jcf.2004.05.046

Sheppard, David N. ; Gray, Michael A. ; Gong, Xiandi ; Sohma, Yoshiro ; Kogan, Ilana ; Benos, Dale J. ; Scott-Ward, Toby S. ; Chen, Jeng Haur ; Li, Hongyu ; Cai, Zhiwei ; Gupta, Jyoti ; Li, Canhui ; Ramjeesingh, Mohabir ; Berdiev, Bakhrom K. ; Ismailov, Iskander I. ; Bear, Christine E. ; Hwang, Tzyh Chang ; Linsdell, Paul ; Hug, Martin J. / The patch-clamp and planar lipid bilayer techniques : Powerful and versatile tools to investigate the CFTR Cl^- channel. In: Journal of Cystic Fibrosis. 2004 ; Vol. 3, No. SUPPL. 2. pp. 101-108.

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abstract = "Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism of action, interaction with other proteins and physiological role. Using the PLB technique, the structure and function of CFTR can be investigated free from the influence of other proteins. Here we discuss how these techniques are employed to investigate the CFTR Cl- channel with special emphasis on its permeation, conduction and gating properties.",

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AU - Cai, Zhiwei

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AU - Li, Canhui

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AU - Berdiev, Bakhrom K.

AU - Ismailov, Iskander I.

AU - Bear, Christine E.

AU - Hwang, Tzyh Chang

AU - Linsdell, Paul

AU - Hug, Martin J.

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