The perivitelline space-forming capacity of mouse oocytes is associated with meiotic competence

Azusa Inoue, Tomohiko Akiyama, Masao Nagata, Fugaku Aoki

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Although mouse oocytes progressively acquire meiotic competence during their growth in the ovaries, only half of full-grown oocytes can accomplish meiosis. Two types of full-grown oocytes have been reported on the basis of their chromatin configuration, the surrounded-nucleolus (SN) type and the non-surrounded-nucleolus (NSN) type. Therefore, full-grown oocytes collected from the ovaries of adult animals comprise a heterogeneous population; some oocytes are meiotically incompetent (NSN-type), and some are competent (SN-type). In the present study, we found that full-grown oocytes could be classified into two groups using the criterion of formation of the perivitelline space (PVS) after culture with 3-isobutyl-1-methylxanthine (IBMX) for 1 h. In oocytes with a PVS, actin-filled processes within zona pellucidae originating from cumulus cells were reduced, while they were rich in oocytes without a PVS, suggesting that a reduction in these processes contributes to PVS formation. PVS formation was highly correlated with meiotic competence and SN-type configuration. The results of this study demonstrate that PVS formation is a useful criterion for easily distinguishing between SN- and NSN-type oocytes, without injury to the cells.

Original languageEnglish
Pages (from-to)1043-1052
Number of pages10
JournalJournal of Reproduction and Development
Volume53
Issue number5
DOIs
Publication statusPublished - 2007 Oct 1
Externally publishedYes

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cell nucleolus
oocytes
mice
zona pellucida
meiosis
actin
chromatin
cells

Keywords

  • Meiosis
  • Mouse
  • Oocyte
  • Perivitelline space

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

The perivitelline space-forming capacity of mouse oocytes is associated with meiotic competence. / Inoue, Azusa; Akiyama, Tomohiko; Nagata, Masao; Aoki, Fugaku.

In: Journal of Reproduction and Development, Vol. 53, No. 5, 01.10.2007, p. 1043-1052.

Research output: Contribution to journalArticle

Inoue, Azusa ; Akiyama, Tomohiko ; Nagata, Masao ; Aoki, Fugaku. / The perivitelline space-forming capacity of mouse oocytes is associated with meiotic competence. In: Journal of Reproduction and Development. 2007 ; Vol. 53, No. 5. pp. 1043-1052.
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