The potential of enriched mesenchymal stem cells with neural crest cell phenotypes as a cell source for regenerative dentistry

Kunimichi Niibe, Maolin Zhang, Kosuke Nakazawa, Satoru Morikawa, Taneaki Nakagawa, Yumi Matsuzaki, Hiroshi Egusa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Effective regenerative treatments for periodontal tissue defects have recently been demonstrated using mesenchymal stromal/stem cells (MSCs). Furthermore, current bioengineering techniques have enabled de novo fabrication of tooth-perio dental units in mice. These cutting-edge technologies are expected to address unmet needs within regenerative dentistry. However, to achieve efficient and stable treatment outcomes, preparation of an appropriate stem cell source is essential. Many researchers are investigating the use of adult stem cells for regenerative dentistry; bone marrow-derived MSCs (BM-MSCs) are particularly promising and presently used clinically. However, current BM-MSC isolation techniques result in a heterogeneous, non-reproducible cell population because of a lack of identified distinct BM-MSC surface markers. Recently, specific subsets of cell surface markers for BM-MSCs have been reported in mice (PDGFRα+ and Sca-1+) and humans (LNGFR+, THY-1+ and VCAM-1+), facilitating the isolation of unique enriched BM-MSCs (so-called "purified MSCs"). Notably, the enriched BM-MSC population contains neural crest-derived cells, which can differentiate into cells of neural crest- and mesenchymal lineages. In this review, characteristics of the enriched BM-MSCs are outlined with a focus on their potential application within future regenerative dentistry.

Original languageEnglish
JournalJapanese Dental Science Review
DOIs
Publication statusAccepted/In press - 2016 Apr 28

Fingerprint

Neural Crest
Dentistry
Mesenchymal Stromal Cells
Bone Marrow
Phenotype
Tooth
Bioengineering
Adult Stem Cells
Vascular Cell Adhesion Molecule-1
Population
Stem Cells
Research Personnel
Technology

Keywords

  • Bone marrow-derived mesenchymal stem cell
  • Enriched/purified mesenchymal stem cell
  • Flow cytometric isolation
  • Neural crest cell
  • Regenerative dentistry

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

The potential of enriched mesenchymal stem cells with neural crest cell phenotypes as a cell source for regenerative dentistry. / Niibe, Kunimichi; Zhang, Maolin; Nakazawa, Kosuke; Morikawa, Satoru; Nakagawa, Taneaki; Matsuzaki, Yumi; Egusa, Hiroshi.

In: Japanese Dental Science Review, 28.04.2016.

Research output: Contribution to journalArticle

@article{904075f0d4984f8db499419e55d83109,
title = "The potential of enriched mesenchymal stem cells with neural crest cell phenotypes as a cell source for regenerative dentistry",
abstract = "Effective regenerative treatments for periodontal tissue defects have recently been demonstrated using mesenchymal stromal/stem cells (MSCs). Furthermore, current bioengineering techniques have enabled de novo fabrication of tooth-perio dental units in mice. These cutting-edge technologies are expected to address unmet needs within regenerative dentistry. However, to achieve efficient and stable treatment outcomes, preparation of an appropriate stem cell source is essential. Many researchers are investigating the use of adult stem cells for regenerative dentistry; bone marrow-derived MSCs (BM-MSCs) are particularly promising and presently used clinically. However, current BM-MSC isolation techniques result in a heterogeneous, non-reproducible cell population because of a lack of identified distinct BM-MSC surface markers. Recently, specific subsets of cell surface markers for BM-MSCs have been reported in mice (PDGFRα+ and Sca-1+) and humans (LNGFR+, THY-1+ and VCAM-1+), facilitating the isolation of unique enriched BM-MSCs (so-called {"}purified MSCs{"}). Notably, the enriched BM-MSC population contains neural crest-derived cells, which can differentiate into cells of neural crest- and mesenchymal lineages. In this review, characteristics of the enriched BM-MSCs are outlined with a focus on their potential application within future regenerative dentistry.",
keywords = "Bone marrow-derived mesenchymal stem cell, Enriched/purified mesenchymal stem cell, Flow cytometric isolation, Neural crest cell, Regenerative dentistry",
author = "Kunimichi Niibe and Maolin Zhang and Kosuke Nakazawa and Satoru Morikawa and Taneaki Nakagawa and Yumi Matsuzaki and Hiroshi Egusa",
year = "2016",
month = "4",
day = "28",
doi = "10.1016/j.jdsr.2016.09.001",
language = "English",
journal = "Japanese Dental Science Review",
issn = "1882-7616",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - The potential of enriched mesenchymal stem cells with neural crest cell phenotypes as a cell source for regenerative dentistry

AU - Niibe, Kunimichi

AU - Zhang, Maolin

AU - Nakazawa, Kosuke

AU - Morikawa, Satoru

AU - Nakagawa, Taneaki

AU - Matsuzaki, Yumi

AU - Egusa, Hiroshi

PY - 2016/4/28

Y1 - 2016/4/28

N2 - Effective regenerative treatments for periodontal tissue defects have recently been demonstrated using mesenchymal stromal/stem cells (MSCs). Furthermore, current bioengineering techniques have enabled de novo fabrication of tooth-perio dental units in mice. These cutting-edge technologies are expected to address unmet needs within regenerative dentistry. However, to achieve efficient and stable treatment outcomes, preparation of an appropriate stem cell source is essential. Many researchers are investigating the use of adult stem cells for regenerative dentistry; bone marrow-derived MSCs (BM-MSCs) are particularly promising and presently used clinically. However, current BM-MSC isolation techniques result in a heterogeneous, non-reproducible cell population because of a lack of identified distinct BM-MSC surface markers. Recently, specific subsets of cell surface markers for BM-MSCs have been reported in mice (PDGFRα+ and Sca-1+) and humans (LNGFR+, THY-1+ and VCAM-1+), facilitating the isolation of unique enriched BM-MSCs (so-called "purified MSCs"). Notably, the enriched BM-MSC population contains neural crest-derived cells, which can differentiate into cells of neural crest- and mesenchymal lineages. In this review, characteristics of the enriched BM-MSCs are outlined with a focus on their potential application within future regenerative dentistry.

AB - Effective regenerative treatments for periodontal tissue defects have recently been demonstrated using mesenchymal stromal/stem cells (MSCs). Furthermore, current bioengineering techniques have enabled de novo fabrication of tooth-perio dental units in mice. These cutting-edge technologies are expected to address unmet needs within regenerative dentistry. However, to achieve efficient and stable treatment outcomes, preparation of an appropriate stem cell source is essential. Many researchers are investigating the use of adult stem cells for regenerative dentistry; bone marrow-derived MSCs (BM-MSCs) are particularly promising and presently used clinically. However, current BM-MSC isolation techniques result in a heterogeneous, non-reproducible cell population because of a lack of identified distinct BM-MSC surface markers. Recently, specific subsets of cell surface markers for BM-MSCs have been reported in mice (PDGFRα+ and Sca-1+) and humans (LNGFR+, THY-1+ and VCAM-1+), facilitating the isolation of unique enriched BM-MSCs (so-called "purified MSCs"). Notably, the enriched BM-MSC population contains neural crest-derived cells, which can differentiate into cells of neural crest- and mesenchymal lineages. In this review, characteristics of the enriched BM-MSCs are outlined with a focus on their potential application within future regenerative dentistry.

KW - Bone marrow-derived mesenchymal stem cell

KW - Enriched/purified mesenchymal stem cell

KW - Flow cytometric isolation

KW - Neural crest cell

KW - Regenerative dentistry

UR - http://www.scopus.com/inward/record.url?scp=85008197530&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85008197530&partnerID=8YFLogxK

U2 - 10.1016/j.jdsr.2016.09.001

DO - 10.1016/j.jdsr.2016.09.001

M3 - Article

AN - SCOPUS:85008197530

JO - Japanese Dental Science Review

JF - Japanese Dental Science Review

SN - 1882-7616

ER -