The potential of vitamin K3 as an anticancer agent against breast cancer that acts via the mitochondria-related apoptotic pathway

Takeshi Akiyoshi, Sumio Matzno, Mika Sakai, Noboru Okamura, Kenji Matsuyama

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Purpose: We tried to clarify the cytotoxic mechanism of VK3 using the breast cancer cell line MCF-7. Methods: Cytotoxicity was measured via intracellular esterase activity. DNA fragmentation was assessed by agarose gel electrophoresis. JC-1 staining was applied to measure mitochondrial dysfunction. Caspase activation and reactive oxidative species (ROS) generation were also measured. Results: VK3 exhibited cytotoxicity that caused DNA fragmentation in MCF-7 cells with an IC50 of 14.2 μM. JC-1 staining revealed that VK3 caused mitochondrial dysfunction including a disappearance of mitochondrial membrane potential. Additional investigation showed that the mitochondrial damage was induced by the generation of ROS and the subsequent activation of caspase-7 and -9. Conclusions: Our findings demonstrate that VK3-induced apoptosis is selectively initiated by the mitochondria-related pathway and might be useful in breast cancer chemotherapy.

Original languageEnglish
Pages (from-to)143-150
Number of pages8
JournalCancer Chemotherapy and Pharmacology
Volume65
Issue number1
DOIs
Publication statusPublished - 2009

Fingerprint

Vitamin K 3
Mitochondria
DNA Fragmentation
Cytotoxicity
Antineoplastic Agents
Chemical activation
Staining and Labeling
Breast Neoplasms
Caspase 7
Chemotherapy
Caspase 9
Agar Gel Electrophoresis
Mitochondrial Membrane Potential
DNA
MCF-7 Cells
Esterases
Caspases
Electrophoresis
Sepharose
Inhibitory Concentration 50

Keywords

  • Breast cancer
  • Mitochondrial dysfunction
  • ROS
  • Vitamin K

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pharmacology
  • Pharmacology (medical)
  • Toxicology

Cite this

The potential of vitamin K3 as an anticancer agent against breast cancer that acts via the mitochondria-related apoptotic pathway. / Akiyoshi, Takeshi; Matzno, Sumio; Sakai, Mika; Okamura, Noboru; Matsuyama, Kenji.

In: Cancer Chemotherapy and Pharmacology, Vol. 65, No. 1, 2009, p. 143-150.

Research output: Contribution to journalArticle

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AU - Matsuyama, Kenji

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