The potential of vitamin K3 as an anticancer agent against breast cancer that acts via the mitochondria-related apoptotic pathway

Takeshi Akiyoshi, Sumio Matzno, Mika Sakai, Noboru Okamura, Kenji Matsuyama

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Purpose: We tried to clarify the cytotoxic mechanism of VK3 using the breast cancer cell line MCF-7. Methods: Cytotoxicity was measured via intracellular esterase activity. DNA fragmentation was assessed by agarose gel electrophoresis. JC-1 staining was applied to measure mitochondrial dysfunction. Caspase activation and reactive oxidative species (ROS) generation were also measured. Results: VK3 exhibited cytotoxicity that caused DNA fragmentation in MCF-7 cells with an IC50 of 14.2 μM. JC-1 staining revealed that VK3 caused mitochondrial dysfunction including a disappearance of mitochondrial membrane potential. Additional investigation showed that the mitochondrial damage was induced by the generation of ROS and the subsequent activation of caspase-7 and -9. Conclusions: Our findings demonstrate that VK3-induced apoptosis is selectively initiated by the mitochondria-related pathway and might be useful in breast cancer chemotherapy.

Original languageEnglish
Pages (from-to)143-150
Number of pages8
JournalCancer Chemotherapy and Pharmacology
Volume65
Issue number1
DOIs
Publication statusPublished - 2009 May 18

Keywords

  • Breast cancer
  • Mitochondrial dysfunction
  • ROS
  • Vitamin K

ASJC Scopus subject areas

  • Oncology
  • Toxicology
  • Pharmacology
  • Cancer Research
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'The potential of vitamin K<sub>3</sub> as an anticancer agent against breast cancer that acts via the mitochondria-related apoptotic pathway'. Together they form a unique fingerprint.

  • Cite this