The promoter of the endo a cytokeratin gene is activated by a 3′ downstream enhancer

Yoshihiro Takemoto, Yasuo Fujimura, Midori Matsumoto, Yoshitaka Tamai, Takashi Morita, Aizo Matsushiro, Masami Nozaki

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Mouse cytokeratin EndoA is an intermediate filament subunit of the type II cytokeratin class which initiates expression in trophectoderm cells of blastocyst during embryogenesis. To identify the regulatory elements of the endo A gene, we constructed a series of CAT expression vectors and transfected them into PYS-2 cells We found an enhancer element locating 1 kb downstream from the endo A gene which acts on both the endo A and SV40 promoters. This enhancer consists of six direct repeated sequences with homology to the PEA3 motif in polyoma virus a enhancer core. In undifferentiated F9 embryonal carcinoma cells, expression of the construct containing the enhancer was not detected. These results indicate that one of the regulatory mechanisms of endo A gene expression is the 3′ downstream enhancer.

Original languageEnglish
Pages (from-to)2761-2765
Number of pages5
JournalNucleic Acids Research
Volume19
Issue number10
Publication statusPublished - 1991 May 25
Externally publishedYes

Fingerprint

Keratins
Promoter
Genes
Gene
Embryonal Carcinoma Stem Cells
Polyomavirus
Intermediate Filaments
Cell
Blastocyst
Sequence Homology
Viruses
Gene expression
Embryonic Development
Embryogenesis
Cells
Filament
Gene Expression
Virus
Mouse
Homology

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Takemoto, Y., Fujimura, Y., Matsumoto, M., Tamai, Y., Morita, T., Matsushiro, A., & Nozaki, M. (1991). The promoter of the endo a cytokeratin gene is activated by a 3′ downstream enhancer. Nucleic Acids Research, 19(10), 2761-2765.

The promoter of the endo a cytokeratin gene is activated by a 3′ downstream enhancer. / Takemoto, Yoshihiro; Fujimura, Yasuo; Matsumoto, Midori; Tamai, Yoshitaka; Morita, Takashi; Matsushiro, Aizo; Nozaki, Masami.

In: Nucleic Acids Research, Vol. 19, No. 10, 25.05.1991, p. 2761-2765.

Research output: Contribution to journalArticle

Takemoto, Y, Fujimura, Y, Matsumoto, M, Tamai, Y, Morita, T, Matsushiro, A & Nozaki, M 1991, 'The promoter of the endo a cytokeratin gene is activated by a 3′ downstream enhancer', Nucleic Acids Research, vol. 19, no. 10, pp. 2761-2765.
Takemoto Y, Fujimura Y, Matsumoto M, Tamai Y, Morita T, Matsushiro A et al. The promoter of the endo a cytokeratin gene is activated by a 3′ downstream enhancer. Nucleic Acids Research. 1991 May 25;19(10):2761-2765.
Takemoto, Yoshihiro ; Fujimura, Yasuo ; Matsumoto, Midori ; Tamai, Yoshitaka ; Morita, Takashi ; Matsushiro, Aizo ; Nozaki, Masami. / The promoter of the endo a cytokeratin gene is activated by a 3′ downstream enhancer. In: Nucleic Acids Research. 1991 ; Vol. 19, No. 10. pp. 2761-2765.
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