The pyrimidine/purine-biased region of the epidermal growth factor receptor gene is sensitive to S1 nuclease and may form an intramolecular triplex

M. Kato, J. Kudoh, N. Shimizu

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The pyrimidine/purine-biased region located upstream of the EGF (epidermal growth factor) receptor gene transcription initiation sites was sensitive to S1 nuclease when under superhelical tension. The structural basis of this specific reactivity to S1 nuclease was probed by the use of diethyl pyrocarbonate. The patterns of modification suggested that the H-form proposed by Mirkin, Lyamichev, Drushlyak, Dobrynin, Fillippov and Frank-Kamenetskii [Nature (London) (1987) 330, 495-497], which includes an intramolecular triplex and a single-stranded region, was the most plausible model for the sequence tested. The results of dimethyl sulphate modification also supported this model.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalBiochemical Journal
Issue number1
Publication statusPublished - 1990 Jan 1


ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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