The ratio of cancer cells to stroma after induction therapy in the treatment of non-small cell lung cancer

Masaki Goto, Masahito Naito, Koichi Saruwatari, Kakeru Hisakane, Motohiro Kojima, Satoshi Fujii, Takeshi Kuwata, Atsushi Ochiai, Shogo Nomura, Keiju Aokage, Tomoyuki Hishida, Junji Yoshida, Kohei Yokoi, Masahiro Tsuboi, Genichiro Ishii

Research output: Contribution to journalArticle

Abstract

Purpose: Induction therapy induces degenerative changes of various degrees in both cancerous and non-cancerous cells of non-small cell lung cancer (NSCLC). The effect of induction therapy on histological characteristics, in particular the ratio of residual cancer cells to non-cancerous components, is unknown. Methods: Seventy-four NSCLC patients treated with induction therapy followed by surgery were enrolled. Residual cancer cells were identified using anti-pan-cytokeratin antibody (AE1/AE3). We analyzed and quantified the following three factors via digital image analysis; (1) the tumor area containing cancer cells and non-cancerous components (TA), (2) the total area of AE1/AE3 positive cancer cells (TACC), (3) the percentage of TACC to TA (%TACC). These factors were also analyzed in a matched control group (surgery alone, n = 80). Results: The median TACC of the induction therapy group was significantly lower than that of the control group (p < 0.01). In addition, the median %TACC of the induction therapy group (5.9 %) was significantly lower than that of the control group (58.6 %) (p < 0.01). TACC had a strong positive correlation with TA in the control group (r = 0.93), but not in the induction therapy group. Conversely, TACC had a strong positive correlation with %TACC in the induction therapy group (r = 0.95), but not in the control group. Conclusion: Unlike the control group, the smaller the total area of residual cancer cells, the higher residual tumor contained non-cancerous components in the induction group, which may be the characteristic histological feature of NSCLC after induction therapy.

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalJournal of Cancer Research and Clinical Oncology
Volume143
Issue number2
DOIs
Publication statusPublished - 2017 Feb 1
Externally publishedYes

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Non-Small Cell Lung Carcinoma
Neoplasms
Residual Neoplasm
Control Groups
Therapeutics
Group Psychotherapy
Cell- and Tissue-Based Therapy
Keratins
Research Design
Antibodies

Keywords

  • Cancer cell
  • Cancer stroma
  • Induction therapy
  • Non-small cell lung cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

The ratio of cancer cells to stroma after induction therapy in the treatment of non-small cell lung cancer. / Goto, Masaki; Naito, Masahito; Saruwatari, Koichi; Hisakane, Kakeru; Kojima, Motohiro; Fujii, Satoshi; Kuwata, Takeshi; Ochiai, Atsushi; Nomura, Shogo; Aokage, Keiju; Hishida, Tomoyuki; Yoshida, Junji; Yokoi, Kohei; Tsuboi, Masahiro; Ishii, Genichiro.

In: Journal of Cancer Research and Clinical Oncology, Vol. 143, No. 2, 01.02.2017, p. 215-223.

Research output: Contribution to journalArticle

Goto, M, Naito, M, Saruwatari, K, Hisakane, K, Kojima, M, Fujii, S, Kuwata, T, Ochiai, A, Nomura, S, Aokage, K, Hishida, T, Yoshida, J, Yokoi, K, Tsuboi, M & Ishii, G 2017, 'The ratio of cancer cells to stroma after induction therapy in the treatment of non-small cell lung cancer', Journal of Cancer Research and Clinical Oncology, vol. 143, no. 2, pp. 215-223. https://doi.org/10.1007/s00432-016-2271-8
Goto, Masaki ; Naito, Masahito ; Saruwatari, Koichi ; Hisakane, Kakeru ; Kojima, Motohiro ; Fujii, Satoshi ; Kuwata, Takeshi ; Ochiai, Atsushi ; Nomura, Shogo ; Aokage, Keiju ; Hishida, Tomoyuki ; Yoshida, Junji ; Yokoi, Kohei ; Tsuboi, Masahiro ; Ishii, Genichiro. / The ratio of cancer cells to stroma after induction therapy in the treatment of non-small cell lung cancer. In: Journal of Cancer Research and Clinical Oncology. 2017 ; Vol. 143, No. 2. pp. 215-223.
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abstract = "Purpose: Induction therapy induces degenerative changes of various degrees in both cancerous and non-cancerous cells of non-small cell lung cancer (NSCLC). The effect of induction therapy on histological characteristics, in particular the ratio of residual cancer cells to non-cancerous components, is unknown. Methods: Seventy-four NSCLC patients treated with induction therapy followed by surgery were enrolled. Residual cancer cells were identified using anti-pan-cytokeratin antibody (AE1/AE3). We analyzed and quantified the following three factors via digital image analysis; (1) the tumor area containing cancer cells and non-cancerous components (TA), (2) the total area of AE1/AE3 positive cancer cells (TACC), (3) the percentage of TACC to TA ({\%}TACC). These factors were also analyzed in a matched control group (surgery alone, n = 80). Results: The median TACC of the induction therapy group was significantly lower than that of the control group (p < 0.01). In addition, the median {\%}TACC of the induction therapy group (5.9 {\%}) was significantly lower than that of the control group (58.6 {\%}) (p < 0.01). TACC had a strong positive correlation with TA in the control group (r = 0.93), but not in the induction therapy group. Conversely, TACC had a strong positive correlation with {\%}TACC in the induction therapy group (r = 0.95), but not in the control group. Conclusion: Unlike the control group, the smaller the total area of residual cancer cells, the higher residual tumor contained non-cancerous components in the induction group, which may be the characteristic histological feature of NSCLC after induction therapy.",
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AU - Goto, Masaki

AU - Naito, Masahito

AU - Saruwatari, Koichi

AU - Hisakane, Kakeru

AU - Kojima, Motohiro

AU - Fujii, Satoshi

AU - Kuwata, Takeshi

AU - Ochiai, Atsushi

AU - Nomura, Shogo

AU - Aokage, Keiju

AU - Hishida, Tomoyuki

AU - Yoshida, Junji

AU - Yokoi, Kohei

AU - Tsuboi, Masahiro

AU - Ishii, Genichiro

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N2 - Purpose: Induction therapy induces degenerative changes of various degrees in both cancerous and non-cancerous cells of non-small cell lung cancer (NSCLC). The effect of induction therapy on histological characteristics, in particular the ratio of residual cancer cells to non-cancerous components, is unknown. Methods: Seventy-four NSCLC patients treated with induction therapy followed by surgery were enrolled. Residual cancer cells were identified using anti-pan-cytokeratin antibody (AE1/AE3). We analyzed and quantified the following three factors via digital image analysis; (1) the tumor area containing cancer cells and non-cancerous components (TA), (2) the total area of AE1/AE3 positive cancer cells (TACC), (3) the percentage of TACC to TA (%TACC). These factors were also analyzed in a matched control group (surgery alone, n = 80). Results: The median TACC of the induction therapy group was significantly lower than that of the control group (p < 0.01). In addition, the median %TACC of the induction therapy group (5.9 %) was significantly lower than that of the control group (58.6 %) (p < 0.01). TACC had a strong positive correlation with TA in the control group (r = 0.93), but not in the induction therapy group. Conversely, TACC had a strong positive correlation with %TACC in the induction therapy group (r = 0.95), but not in the control group. Conclusion: Unlike the control group, the smaller the total area of residual cancer cells, the higher residual tumor contained non-cancerous components in the induction group, which may be the characteristic histological feature of NSCLC after induction therapy.

AB - Purpose: Induction therapy induces degenerative changes of various degrees in both cancerous and non-cancerous cells of non-small cell lung cancer (NSCLC). The effect of induction therapy on histological characteristics, in particular the ratio of residual cancer cells to non-cancerous components, is unknown. Methods: Seventy-four NSCLC patients treated with induction therapy followed by surgery were enrolled. Residual cancer cells were identified using anti-pan-cytokeratin antibody (AE1/AE3). We analyzed and quantified the following three factors via digital image analysis; (1) the tumor area containing cancer cells and non-cancerous components (TA), (2) the total area of AE1/AE3 positive cancer cells (TACC), (3) the percentage of TACC to TA (%TACC). These factors were also analyzed in a matched control group (surgery alone, n = 80). Results: The median TACC of the induction therapy group was significantly lower than that of the control group (p < 0.01). In addition, the median %TACC of the induction therapy group (5.9 %) was significantly lower than that of the control group (58.6 %) (p < 0.01). TACC had a strong positive correlation with TA in the control group (r = 0.93), but not in the induction therapy group. Conversely, TACC had a strong positive correlation with %TACC in the induction therapy group (r = 0.95), but not in the control group. Conclusion: Unlike the control group, the smaller the total area of residual cancer cells, the higher residual tumor contained non-cancerous components in the induction group, which may be the characteristic histological feature of NSCLC after induction therapy.

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KW - Cancer stroma

KW - Induction therapy

KW - Non-small cell lung cancer

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