The reeler gene-associated antigen on cajal-retzius neurons is a crucial molecule for laminar organization of cortical neurons

Masaharu Ogawa, Takaki Miyata, Kazunori Nakajima, Kenichi Yagyu, Masahiro Seike, Kazuhiro Ikenaka, Hiroshi Yamamoto, Katsuhiko Mikoshibat

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Abstract

In the neurological mutant mouse reeler, the histological organization of the neocortex develops abnormally and essentially results in an inversion of the relative positions of the cortical layers. The reeler mutation, therefore, provides an insight into the molecular mechanisms underlying the formation of the cortical layers. We have generated a monoclonal antibody (CR-50) that probes a distinct allelic antigen present in wild-type but not in reeler mutant mice. CR-50 reacted specifically with Cajal-Retzius neurons, one of the first cortical neurons to differentiate in the neocortex, but whose functional role is not known. When dissociated cerebral cortical cells were incubated with CR-50 in reaggregation culture, the genotype-dependent histogenetic assembly of wild-type cortical cells resembled that of reeler mutants. These findings revealed that the selective expression of a distinct molecule on Cajal-Retzius neurons is critical for the normal lamination of cortical neurons in the mammalian neocortex.

Original languageEnglish
Pages (from-to)899-912
Number of pages14
JournalNeuron
Volume14
Issue number5
DOIs
Publication statusPublished - 1995

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Neocortex
Neurologic Mutant Mice
Neurons
Antigens
Genes
Genotype
Monoclonal Antibodies
Mutation

ASJC Scopus subject areas

  • Neuroscience(all)

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The reeler gene-associated antigen on cajal-retzius neurons is a crucial molecule for laminar organization of cortical neurons. / Ogawa, Masaharu; Miyata, Takaki; Nakajima, Kazunori; Yagyu, Kenichi; Seike, Masahiro; Ikenaka, Kazuhiro; Yamamoto, Hiroshi; Mikoshibat, Katsuhiko.

In: Neuron, Vol. 14, No. 5, 1995, p. 899-912.

Research output: Contribution to journalArticle

Ogawa, Masaharu ; Miyata, Takaki ; Nakajima, Kazunori ; Yagyu, Kenichi ; Seike, Masahiro ; Ikenaka, Kazuhiro ; Yamamoto, Hiroshi ; Mikoshibat, Katsuhiko. / The reeler gene-associated antigen on cajal-retzius neurons is a crucial molecule for laminar organization of cortical neurons. In: Neuron. 1995 ; Vol. 14, No. 5. pp. 899-912.
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