The role of calcium in platelet microtubule assembly-disassembly

Y. Ikeda, M. Kikuchi, M. Handa, Y. Yoshii, K. Toyama, M. Yamamoto, K. Watanabe, Y. Ando

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The role of calcium in the equilibrium between assembled and disassembled microtubules was investigated in human platelets. The two pools of microtubule protein was "frozen" by addition of a glycerol-dimethyl sulfoxide-containing medium and were estimated by measuring the colchicine binding activities of total and polymerized tubulin. Addition of A23187 to platelet rich plasma produced a transient decrease in the pool of polymerized tubulin within 30 sec., followed by a return to base-line values within 2 min.. Total content of platelet tubulin remained unchanged during the course of aggregation. TMB-8, a known intracellular calcium antagonist, abolished this transient decrease in polymerized tubulin induced by A23187, while indomethacin did not. These findings strongly suggest the important role of intracellular calcium in microtubule assembly-disassembly.

Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalThrombosis Research
Volume23
Issue number1-2
DOIs
Publication statusPublished - 1981

Fingerprint

Tubulin
Microtubules
Blood Platelets
Calcium
Calcimycin
Microtubule Proteins
Platelet-Rich Plasma
Colchicine
Dimethyl Sulfoxide
Indomethacin
Glycerol

Keywords

  • calcium ionophore
  • intracellular calcium
  • Microtubules
  • tubulin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Ikeda, Y., Kikuchi, M., Handa, M., Yoshii, Y., Toyama, K., Yamamoto, M., ... Ando, Y. (1981). The role of calcium in platelet microtubule assembly-disassembly. Thrombosis Research, 23(1-2), 163-168. https://doi.org/10.1016/0049-3848(81)90248-6

The role of calcium in platelet microtubule assembly-disassembly. / Ikeda, Y.; Kikuchi, M.; Handa, M.; Yoshii, Y.; Toyama, K.; Yamamoto, M.; Watanabe, K.; Ando, Y.

In: Thrombosis Research, Vol. 23, No. 1-2, 1981, p. 163-168.

Research output: Contribution to journalArticle

Ikeda, Y, Kikuchi, M, Handa, M, Yoshii, Y, Toyama, K, Yamamoto, M, Watanabe, K & Ando, Y 1981, 'The role of calcium in platelet microtubule assembly-disassembly', Thrombosis Research, vol. 23, no. 1-2, pp. 163-168. https://doi.org/10.1016/0049-3848(81)90248-6
Ikeda Y, Kikuchi M, Handa M, Yoshii Y, Toyama K, Yamamoto M et al. The role of calcium in platelet microtubule assembly-disassembly. Thrombosis Research. 1981;23(1-2):163-168. https://doi.org/10.1016/0049-3848(81)90248-6
Ikeda, Y. ; Kikuchi, M. ; Handa, M. ; Yoshii, Y. ; Toyama, K. ; Yamamoto, M. ; Watanabe, K. ; Ando, Y. / The role of calcium in platelet microtubule assembly-disassembly. In: Thrombosis Research. 1981 ; Vol. 23, No. 1-2. pp. 163-168.
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