The Role of Catalase in Pulmonary Fibrosis

Nao Odajima, Tomoko Betsuyaku, Katsura Nagai, Chinatsu Moriyama, Da Hong Wang, Tomoko Takigawa, Keiki Ogino, Masaharu Nishimura

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Catalase is preferentially expressed in bronchiolar and alveolar epithelial cells, and acts as an endogenous antioxidant enzyme in normal lungs. We thus postulated epithelial damage would be associated with a functional deficiency of catalase during the development of lung fibrosis.Methods: The present study evaluates the expression of catalase mRNA and protein in human interstitial pneumonias and in mouse bleomycin-induced lung injury. We examined the degree of bleomycin-induced inflammation and fibrosis in the mice with lowered catalase activity.Results: In humans, catalase was decreased at the levels of activity, protein content and mRNA expression in fibrotic lungs (n = 12) compared to control lungs (n = 10). Immunohistochemistry revealed a decrease in catalase in bronchiolar epithelium and abnormal re-epithelialization in fibrotic areas. In C57BL/6J mice, catalase activity was suppressed along with downregulation of catalase mRNA in whole lung homogenates after bleomycin administration. In acatalasemic mice, neutrophilic inflammation was prolonged until 14 days, and there was a higher degree of lung fibrosis in association with a higher level of transforming growth factor-β expression and total collagen content following bleomycin treatment compared to wild-type mice.Conclusions: Taken together, these findings demonstrate diminished catalase expression and activity in human pulmonary fibrosis and suggest the protective role of catalase against bleomycin-induced inflammation and subsequent fibrosis.

Original languageEnglish
Article number183
JournalRespiratory Research
Volume11
DOIs
Publication statusPublished - 2010 Dec 29
Externally publishedYes

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Pulmonary Fibrosis
Catalase
Bleomycin
Lung
Fibrosis
Inflammation
Messenger RNA
Acatalasia
Re-Epithelialization
Alveolar Epithelial Cells
Interstitial Lung Diseases
Lung Injury
Transforming Growth Factors
Inbred C57BL Mouse
Human Activities
Proteins
Collagen
Down-Regulation
Epithelium
Antioxidants

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Odajima, N., Betsuyaku, T., Nagai, K., Moriyama, C., Wang, D. H., Takigawa, T., ... Nishimura, M. (2010). The Role of Catalase in Pulmonary Fibrosis. Respiratory Research, 11, [183]. https://doi.org/10.1186/1465-9921-11-183

The Role of Catalase in Pulmonary Fibrosis. / Odajima, Nao; Betsuyaku, Tomoko; Nagai, Katsura; Moriyama, Chinatsu; Wang, Da Hong; Takigawa, Tomoko; Ogino, Keiki; Nishimura, Masaharu.

In: Respiratory Research, Vol. 11, 183, 29.12.2010.

Research output: Contribution to journalArticle

Odajima, N, Betsuyaku, T, Nagai, K, Moriyama, C, Wang, DH, Takigawa, T, Ogino, K & Nishimura, M 2010, 'The Role of Catalase in Pulmonary Fibrosis', Respiratory Research, vol. 11, 183. https://doi.org/10.1186/1465-9921-11-183
Odajima N, Betsuyaku T, Nagai K, Moriyama C, Wang DH, Takigawa T et al. The Role of Catalase in Pulmonary Fibrosis. Respiratory Research. 2010 Dec 29;11. 183. https://doi.org/10.1186/1465-9921-11-183
Odajima, Nao ; Betsuyaku, Tomoko ; Nagai, Katsura ; Moriyama, Chinatsu ; Wang, Da Hong ; Takigawa, Tomoko ; Ogino, Keiki ; Nishimura, Masaharu. / The Role of Catalase in Pulmonary Fibrosis. In: Respiratory Research. 2010 ; Vol. 11.
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