The role of fractalkine as an accelerating factor on the autoimmune exocrinopathy in mice

Kazuo Tsubota, Tatsuaki Nishiyama, Kenji Mishima, Hiroko Inoue, Takeshi Doi, Yukio Hattori, Tatsuhiko Kodama, Akihiro Higuchi, Yoshio Hayashi, Ichiro Saito

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

PURPOSE. Sjögren's syndrome (SS) is an organ-specific autoimmune disease caused by the progressive loss of exocrine glands and is associated with several autoimmune phenomena. Various research studies have been performed, and many molecules have been suggested as responsible for the pathogenesis of SS. Here the authors show the increased expression of fractalkine (CX3CL1) in lacrimal glands of SS model mice. Among more than 50 known chemokines, fractalkine is the sole member of the CX3C family and has unique structural and functional attributes. The purpose of this study was to analyze the role of fractalkine in exocrine glands. METHODS. The expression of fractalkine in the lacrimal glands of thymectomized NFS/sld mice was investigated by immunohistochemistry and RT-PCR. To confirm the effects of fractalkine in exocrine glands, tissue-specific fractalkine transgenic mice were generated using the salivary amylase promoter. RESULTS. The results demonstrated the upregulated fractalkine expression in thymectomized NFS/sld mice. Furthermore, the lacrimal and salivary gland-specific fractalkine transgenic mice showed the expression of fragmented fractalkine and lymphocytic infiltration in their lacrimal and submandibular glands. Interestingly, the dominant population was B cells in the lacrimal glands, whereas B cells and CD4+ T cells were infiltrated in the submandibular glands. These mice also demonstrated slightly decreased tear and salivary secretion compared with wild-type mice. CONCLUSIONS. Based on these results, it may be that fractalkine contributes to the development of SS, especially in lymphocyte migration to exocrine glands, and that it accelerates the disease in association with other molecules.

Original languageEnglish
Pages (from-to)4753-4760
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume50
Issue number10
DOIs
Publication statusPublished - 2009

Fingerprint

Chemokine CX3CL1
Lacrimal Apparatus
Exocrine Glands
Submandibular Gland
Transgenic Mice
B-Lymphocytes
Amylases
Salivary Glands
Tears
Chemokines
Autoimmune Diseases
Immunohistochemistry

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

The role of fractalkine as an accelerating factor on the autoimmune exocrinopathy in mice. / Tsubota, Kazuo; Nishiyama, Tatsuaki; Mishima, Kenji; Inoue, Hiroko; Doi, Takeshi; Hattori, Yukio; Kodama, Tatsuhiko; Higuchi, Akihiro; Hayashi, Yoshio; Saito, Ichiro.

In: Investigative Ophthalmology and Visual Science, Vol. 50, No. 10, 2009, p. 4753-4760.

Research output: Contribution to journalArticle

Tsubota, K, Nishiyama, T, Mishima, K, Inoue, H, Doi, T, Hattori, Y, Kodama, T, Higuchi, A, Hayashi, Y & Saito, I 2009, 'The role of fractalkine as an accelerating factor on the autoimmune exocrinopathy in mice', Investigative Ophthalmology and Visual Science, vol. 50, no. 10, pp. 4753-4760. https://doi.org/10.1167/iovs.08-2596
Tsubota, Kazuo ; Nishiyama, Tatsuaki ; Mishima, Kenji ; Inoue, Hiroko ; Doi, Takeshi ; Hattori, Yukio ; Kodama, Tatsuhiko ; Higuchi, Akihiro ; Hayashi, Yoshio ; Saito, Ichiro. / The role of fractalkine as an accelerating factor on the autoimmune exocrinopathy in mice. In: Investigative Ophthalmology and Visual Science. 2009 ; Vol. 50, No. 10. pp. 4753-4760.
@article{62f70da0a5ba4f7a929fe598d4804535,
title = "The role of fractalkine as an accelerating factor on the autoimmune exocrinopathy in mice",
abstract = "PURPOSE. Sj{\"o}gren's syndrome (SS) is an organ-specific autoimmune disease caused by the progressive loss of exocrine glands and is associated with several autoimmune phenomena. Various research studies have been performed, and many molecules have been suggested as responsible for the pathogenesis of SS. Here the authors show the increased expression of fractalkine (CX3CL1) in lacrimal glands of SS model mice. Among more than 50 known chemokines, fractalkine is the sole member of the CX3C family and has unique structural and functional attributes. The purpose of this study was to analyze the role of fractalkine in exocrine glands. METHODS. The expression of fractalkine in the lacrimal glands of thymectomized NFS/sld mice was investigated by immunohistochemistry and RT-PCR. To confirm the effects of fractalkine in exocrine glands, tissue-specific fractalkine transgenic mice were generated using the salivary amylase promoter. RESULTS. The results demonstrated the upregulated fractalkine expression in thymectomized NFS/sld mice. Furthermore, the lacrimal and salivary gland-specific fractalkine transgenic mice showed the expression of fragmented fractalkine and lymphocytic infiltration in their lacrimal and submandibular glands. Interestingly, the dominant population was B cells in the lacrimal glands, whereas B cells and CD4+ T cells were infiltrated in the submandibular glands. These mice also demonstrated slightly decreased tear and salivary secretion compared with wild-type mice. CONCLUSIONS. Based on these results, it may be that fractalkine contributes to the development of SS, especially in lymphocyte migration to exocrine glands, and that it accelerates the disease in association with other molecules.",
author = "Kazuo Tsubota and Tatsuaki Nishiyama and Kenji Mishima and Hiroko Inoue and Takeshi Doi and Yukio Hattori and Tatsuhiko Kodama and Akihiro Higuchi and Yoshio Hayashi and Ichiro Saito",
year = "2009",
doi = "10.1167/iovs.08-2596",
language = "English",
volume = "50",
pages = "4753--4760",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "10",

}

TY - JOUR

T1 - The role of fractalkine as an accelerating factor on the autoimmune exocrinopathy in mice

AU - Tsubota, Kazuo

AU - Nishiyama, Tatsuaki

AU - Mishima, Kenji

AU - Inoue, Hiroko

AU - Doi, Takeshi

AU - Hattori, Yukio

AU - Kodama, Tatsuhiko

AU - Higuchi, Akihiro

AU - Hayashi, Yoshio

AU - Saito, Ichiro

PY - 2009

Y1 - 2009

N2 - PURPOSE. Sjögren's syndrome (SS) is an organ-specific autoimmune disease caused by the progressive loss of exocrine glands and is associated with several autoimmune phenomena. Various research studies have been performed, and many molecules have been suggested as responsible for the pathogenesis of SS. Here the authors show the increased expression of fractalkine (CX3CL1) in lacrimal glands of SS model mice. Among more than 50 known chemokines, fractalkine is the sole member of the CX3C family and has unique structural and functional attributes. The purpose of this study was to analyze the role of fractalkine in exocrine glands. METHODS. The expression of fractalkine in the lacrimal glands of thymectomized NFS/sld mice was investigated by immunohistochemistry and RT-PCR. To confirm the effects of fractalkine in exocrine glands, tissue-specific fractalkine transgenic mice were generated using the salivary amylase promoter. RESULTS. The results demonstrated the upregulated fractalkine expression in thymectomized NFS/sld mice. Furthermore, the lacrimal and salivary gland-specific fractalkine transgenic mice showed the expression of fragmented fractalkine and lymphocytic infiltration in their lacrimal and submandibular glands. Interestingly, the dominant population was B cells in the lacrimal glands, whereas B cells and CD4+ T cells were infiltrated in the submandibular glands. These mice also demonstrated slightly decreased tear and salivary secretion compared with wild-type mice. CONCLUSIONS. Based on these results, it may be that fractalkine contributes to the development of SS, especially in lymphocyte migration to exocrine glands, and that it accelerates the disease in association with other molecules.

AB - PURPOSE. Sjögren's syndrome (SS) is an organ-specific autoimmune disease caused by the progressive loss of exocrine glands and is associated with several autoimmune phenomena. Various research studies have been performed, and many molecules have been suggested as responsible for the pathogenesis of SS. Here the authors show the increased expression of fractalkine (CX3CL1) in lacrimal glands of SS model mice. Among more than 50 known chemokines, fractalkine is the sole member of the CX3C family and has unique structural and functional attributes. The purpose of this study was to analyze the role of fractalkine in exocrine glands. METHODS. The expression of fractalkine in the lacrimal glands of thymectomized NFS/sld mice was investigated by immunohistochemistry and RT-PCR. To confirm the effects of fractalkine in exocrine glands, tissue-specific fractalkine transgenic mice were generated using the salivary amylase promoter. RESULTS. The results demonstrated the upregulated fractalkine expression in thymectomized NFS/sld mice. Furthermore, the lacrimal and salivary gland-specific fractalkine transgenic mice showed the expression of fragmented fractalkine and lymphocytic infiltration in their lacrimal and submandibular glands. Interestingly, the dominant population was B cells in the lacrimal glands, whereas B cells and CD4+ T cells were infiltrated in the submandibular glands. These mice also demonstrated slightly decreased tear and salivary secretion compared with wild-type mice. CONCLUSIONS. Based on these results, it may be that fractalkine contributes to the development of SS, especially in lymphocyte migration to exocrine glands, and that it accelerates the disease in association with other molecules.

UR - http://www.scopus.com/inward/record.url?scp=70349568520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349568520&partnerID=8YFLogxK

U2 - 10.1167/iovs.08-2596

DO - 10.1167/iovs.08-2596

M3 - Article

VL - 50

SP - 4753

EP - 4760

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 10

ER -