The Role of NF-κB Signaling in the Maintenance of Pluripotency of Human Induced Pluripotent Stem Cells

Osamu Takase, Masahiro Yoshikawa, Mana Idei, Junichi Hirahashi, Toshiro Fujita, Tsuyoshi Takato, Takayuki Isagawa, Genta Nagae, Hirofumi Suemori, Hiroyuki Aburatani, Keiichi Hishikawa

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20 Citations (Scopus)

Abstract

NF-κB signaling plays an essential role in maintaining the undifferentiated state of embryonic stem (ES) cells. However, opposing roles of NF-κB have been reported in mouse and human ES cells, and the role of NF-κB in human induced pluripotent stem (iPS) cells has not yet been clarified. Here, we report the role of NF-κB signaling in maintaining the undifferentiated state of human iPS cells. Compared with differentiated cells, undifferentiated human iPS cells showed an augmentation of NF-κB activity. During differentiation induced by the removal of feeder cells and FGF2, we observed a reduction in NF-κB activity, the expression of the undifferentiation markers Oct3/4 and Nanog, and the up-regulation of the differentiated markers WT-1 and Pax-2. The specific knockdown of NF-κB signaling using p65 siRNA also reduced the expression of Oct3/4 and Nanog and up-regulated WT-1 and Pax-2 but did not change the ES-like colony formation. Our results show that the augmentation of NF-κB signaling maintains the undifferentiated state of human iPS and suggest the importance of this signaling pathway in maintenance of human iPS cells.

Original languageEnglish
Article numbere56399
JournalPloS one
Volume8
Issue number2
DOIs
Publication statusPublished - 2013 Feb 20
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Takase, O., Yoshikawa, M., Idei, M., Hirahashi, J., Fujita, T., Takato, T., Isagawa, T., Nagae, G., Suemori, H., Aburatani, H., & Hishikawa, K. (2013). The Role of NF-κB Signaling in the Maintenance of Pluripotency of Human Induced Pluripotent Stem Cells. PloS one, 8(2), [e56399]. https://doi.org/10.1371/journal.pone.0056399