TY - JOUR
T1 - The Role of NF-κB Signaling in the Maintenance of Pluripotency of Human Induced Pluripotent Stem Cells
AU - Takase, Osamu
AU - Yoshikawa, Masahiro
AU - Idei, Mana
AU - Hirahashi, Junichi
AU - Fujita, Toshiro
AU - Takato, Tsuyoshi
AU - Isagawa, Takayuki
AU - Nagae, Genta
AU - Suemori, Hirofumi
AU - Aburatani, Hiroyuki
AU - Hishikawa, Keiichi
N1 - Funding Information:
This work was supported, in part, by Mochida Pharmaceutical Co. Ltd. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials.
PY - 2013/2/20
Y1 - 2013/2/20
N2 - NF-κB signaling plays an essential role in maintaining the undifferentiated state of embryonic stem (ES) cells. However, opposing roles of NF-κB have been reported in mouse and human ES cells, and the role of NF-κB in human induced pluripotent stem (iPS) cells has not yet been clarified. Here, we report the role of NF-κB signaling in maintaining the undifferentiated state of human iPS cells. Compared with differentiated cells, undifferentiated human iPS cells showed an augmentation of NF-κB activity. During differentiation induced by the removal of feeder cells and FGF2, we observed a reduction in NF-κB activity, the expression of the undifferentiation markers Oct3/4 and Nanog, and the up-regulation of the differentiated markers WT-1 and Pax-2. The specific knockdown of NF-κB signaling using p65 siRNA also reduced the expression of Oct3/4 and Nanog and up-regulated WT-1 and Pax-2 but did not change the ES-like colony formation. Our results show that the augmentation of NF-κB signaling maintains the undifferentiated state of human iPS and suggest the importance of this signaling pathway in maintenance of human iPS cells.
AB - NF-κB signaling plays an essential role in maintaining the undifferentiated state of embryonic stem (ES) cells. However, opposing roles of NF-κB have been reported in mouse and human ES cells, and the role of NF-κB in human induced pluripotent stem (iPS) cells has not yet been clarified. Here, we report the role of NF-κB signaling in maintaining the undifferentiated state of human iPS cells. Compared with differentiated cells, undifferentiated human iPS cells showed an augmentation of NF-κB activity. During differentiation induced by the removal of feeder cells and FGF2, we observed a reduction in NF-κB activity, the expression of the undifferentiation markers Oct3/4 and Nanog, and the up-regulation of the differentiated markers WT-1 and Pax-2. The specific knockdown of NF-κB signaling using p65 siRNA also reduced the expression of Oct3/4 and Nanog and up-regulated WT-1 and Pax-2 but did not change the ES-like colony formation. Our results show that the augmentation of NF-κB signaling maintains the undifferentiated state of human iPS and suggest the importance of this signaling pathway in maintenance of human iPS cells.
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U2 - 10.1371/journal.pone.0056399
DO - 10.1371/journal.pone.0056399
M3 - Article
C2 - 23437124
AN - SCOPUS:84874256668
SN - 1932-6203
VL - 8
JO - PLoS One
JF - PLoS One
IS - 2
M1 - e56399
ER -