The role of oxidative stress and inflammation in conjunctivochalasis

Samantha K. Ward, Tais Hitomi Wakamatsu, Murat Dogru, Osama M A Ibrahim, Minako Kaido, Yoko Ogawa, Yukihiro Matsumoto, Ayako Igarashi, Reiko Ishida, Jun Shimazaki, Cristina Schnider, Kazuno Negishi, Chikako Katakami, Kazuo Tsubota

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

PURPOSE. To investigate the status of oxidative stress and histopathologic alterations in patients with conjunctivochalasis and compare the findings with those in healthy control subjects. METHODS. Eleven patients (n = 20 eyes) with Yokoi grade 3 conjunctivochalasis and 11 health control subjects (n = 22 eyes) were prospectively recruited. ELISA for tear hexanoyllysine (HEL) and inflammatory cytokines, tear film break-up time tests, Schirmer test measurements, and fluorescein and rose bengal vital staining were performed. Conjunctival specimens obtained during surgery for conjunctivochalasis and cataract underwent immunohistochemical staining for HEL+8- OHdG, MMP-3, and MMP-9, and positively stained cells were counted. Transmission electron microscopy was also performed, with staining for elastic fibers in the conjunctival stroma. RESULTS. The mean tear stability and vital staining scores were significantly worse in the conjunctivochalasis patients than in the control subjects. The tear HEL and tear cytokine levels showed significantly higher values in eyes with conjunctivochalasis. IL-1β and IL-6 levels showed a significant correlation with corneal epithelial damage. IL-1β and TNFα showed a significant correlation with 8-OHdG-stained cell counts. Specimens from patients with conjunctivochalasis revealed a significantly higher number of cells positively stained for HEL, 8-OHdG, MMP-3, and MMP-9 than did specimens from age- and sex-matched control subjects. Transmission electron microscopy showed decreased intercellular cohesiveness, with the conjunctival stroma showing an accumulation of elastic fibers. CONCLUSIONS. Lipid and DNA oxidative stress were present in the conjunctiva. Increased tear inflammation seemed to coexist with loss of conjunctival epithelial cohesiveness and increased collagenolytic activity, which may explain the conjunctival laxity observed in patients with conjunctivochalasis.

Original languageEnglish
Pages (from-to)1994-2002
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume51
Issue number4
DOIs
Publication statusPublished - 2010 Apr

Fingerprint

Tears
Oxidative Stress
Inflammation
Matrix Metalloproteinases
Staining and Labeling
Elastic Tissue
Transmission Electron Microscopy
Interleukin-1
Cell Count
Cytokines
Rose Bengal
Conjunctiva
Fluorescein
Cataract
Interleukin-6
Healthy Volunteers
Enzyme-Linked Immunosorbent Assay
Lipids
DNA
Health

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Ward, S. K., Wakamatsu, T. H., Dogru, M., Ibrahim, O. M. A., Kaido, M., Ogawa, Y., ... Tsubota, K. (2010). The role of oxidative stress and inflammation in conjunctivochalasis. Investigative Ophthalmology and Visual Science, 51(4), 1994-2002. https://doi.org/10.1167/iovs.09-4130

The role of oxidative stress and inflammation in conjunctivochalasis. / Ward, Samantha K.; Wakamatsu, Tais Hitomi; Dogru, Murat; Ibrahim, Osama M A; Kaido, Minako; Ogawa, Yoko; Matsumoto, Yukihiro; Igarashi, Ayako; Ishida, Reiko; Shimazaki, Jun; Schnider, Cristina; Negishi, Kazuno; Katakami, Chikako; Tsubota, Kazuo.

In: Investigative Ophthalmology and Visual Science, Vol. 51, No. 4, 04.2010, p. 1994-2002.

Research output: Contribution to journalArticle

Ward, SK, Wakamatsu, TH, Dogru, M, Ibrahim, OMA, Kaido, M, Ogawa, Y, Matsumoto, Y, Igarashi, A, Ishida, R, Shimazaki, J, Schnider, C, Negishi, K, Katakami, C & Tsubota, K 2010, 'The role of oxidative stress and inflammation in conjunctivochalasis', Investigative Ophthalmology and Visual Science, vol. 51, no. 4, pp. 1994-2002. https://doi.org/10.1167/iovs.09-4130
Ward, Samantha K. ; Wakamatsu, Tais Hitomi ; Dogru, Murat ; Ibrahim, Osama M A ; Kaido, Minako ; Ogawa, Yoko ; Matsumoto, Yukihiro ; Igarashi, Ayako ; Ishida, Reiko ; Shimazaki, Jun ; Schnider, Cristina ; Negishi, Kazuno ; Katakami, Chikako ; Tsubota, Kazuo. / The role of oxidative stress and inflammation in conjunctivochalasis. In: Investigative Ophthalmology and Visual Science. 2010 ; Vol. 51, No. 4. pp. 1994-2002.
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AU - Kaido, Minako

AU - Ogawa, Yoko

AU - Matsumoto, Yukihiro

AU - Igarashi, Ayako

AU - Ishida, Reiko

AU - Shimazaki, Jun

AU - Schnider, Cristina

AU - Negishi, Kazuno

AU - Katakami, Chikako

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N2 - PURPOSE. To investigate the status of oxidative stress and histopathologic alterations in patients with conjunctivochalasis and compare the findings with those in healthy control subjects. METHODS. Eleven patients (n = 20 eyes) with Yokoi grade 3 conjunctivochalasis and 11 health control subjects (n = 22 eyes) were prospectively recruited. ELISA for tear hexanoyllysine (HEL) and inflammatory cytokines, tear film break-up time tests, Schirmer test measurements, and fluorescein and rose bengal vital staining were performed. Conjunctival specimens obtained during surgery for conjunctivochalasis and cataract underwent immunohistochemical staining for HEL+8- OHdG, MMP-3, and MMP-9, and positively stained cells were counted. Transmission electron microscopy was also performed, with staining for elastic fibers in the conjunctival stroma. RESULTS. The mean tear stability and vital staining scores were significantly worse in the conjunctivochalasis patients than in the control subjects. The tear HEL and tear cytokine levels showed significantly higher values in eyes with conjunctivochalasis. IL-1β and IL-6 levels showed a significant correlation with corneal epithelial damage. IL-1β and TNFα showed a significant correlation with 8-OHdG-stained cell counts. Specimens from patients with conjunctivochalasis revealed a significantly higher number of cells positively stained for HEL, 8-OHdG, MMP-3, and MMP-9 than did specimens from age- and sex-matched control subjects. Transmission electron microscopy showed decreased intercellular cohesiveness, with the conjunctival stroma showing an accumulation of elastic fibers. CONCLUSIONS. Lipid and DNA oxidative stress were present in the conjunctiva. Increased tear inflammation seemed to coexist with loss of conjunctival epithelial cohesiveness and increased collagenolytic activity, which may explain the conjunctival laxity observed in patients with conjunctivochalasis.

AB - PURPOSE. To investigate the status of oxidative stress and histopathologic alterations in patients with conjunctivochalasis and compare the findings with those in healthy control subjects. METHODS. Eleven patients (n = 20 eyes) with Yokoi grade 3 conjunctivochalasis and 11 health control subjects (n = 22 eyes) were prospectively recruited. ELISA for tear hexanoyllysine (HEL) and inflammatory cytokines, tear film break-up time tests, Schirmer test measurements, and fluorescein and rose bengal vital staining were performed. Conjunctival specimens obtained during surgery for conjunctivochalasis and cataract underwent immunohistochemical staining for HEL+8- OHdG, MMP-3, and MMP-9, and positively stained cells were counted. Transmission electron microscopy was also performed, with staining for elastic fibers in the conjunctival stroma. RESULTS. The mean tear stability and vital staining scores were significantly worse in the conjunctivochalasis patients than in the control subjects. The tear HEL and tear cytokine levels showed significantly higher values in eyes with conjunctivochalasis. IL-1β and IL-6 levels showed a significant correlation with corneal epithelial damage. IL-1β and TNFα showed a significant correlation with 8-OHdG-stained cell counts. Specimens from patients with conjunctivochalasis revealed a significantly higher number of cells positively stained for HEL, 8-OHdG, MMP-3, and MMP-9 than did specimens from age- and sex-matched control subjects. Transmission electron microscopy showed decreased intercellular cohesiveness, with the conjunctival stroma showing an accumulation of elastic fibers. CONCLUSIONS. Lipid and DNA oxidative stress were present in the conjunctiva. Increased tear inflammation seemed to coexist with loss of conjunctival epithelial cohesiveness and increased collagenolytic activity, which may explain the conjunctival laxity observed in patients with conjunctivochalasis.

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