The role of Staphylococcal enterotoxin in atopic keratoconjunctivitis and corneal ulceration

H. Fujishima, N. Okada, M. Dogru, F. Baba, Masaru Tomita, J. Abe, K. Matsumoto, H. Saito

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background Patients with atopic eczema frequently experience colonization with Staphylococcus aureus that is directly correlated with the eczema severity. We hypothesized that S. aureus-secreted enterotoxins (SE) are involved in the pathophysiology of atopic keratoconjunctivitis (AKC). Methods A total of 45 subjects (18 with AKC, nine vernal keratoconjunctivitis (VKC), eight seasonal allergic conjunctivitis (SAC), and ten healthy volunteers) were enrolled. Slit lamp examinations, including fluorescein staining, were performed. Scraped samples were collected from the upper tarsal conjunctiva, lower conjunctival sacs, and the skin around the eyelid margins. Superantigen (SAg) genes were detected using polymerase chain reaction (PCR). Results Among 45 cases, S. aureus was detected significantly more in AKC patients than VKC patients (P = 0.026), SAC patients (P = 0.0003), and healthy volunteers (P = 0.0001). SAg genes were detected in 11 patients. SEB (2/11), SEG (8/11), and SEI (8/11) were detected, but no other SE. There was a significant difference in SE detection between AKC and SAC patients (P = 0.03). In severe types of ocular allergic disease such as AKC and VKC (N = 27), SE was detected in six of ten patients with corneal ulcers and two of 17 patients without corneal ulcers. SE was detected in significantly more patients with corneal ulcers (P = 0.025). Conclusions In patients with AKC, S. aureus and SE were detected more frequently compared with other patients and healthy volunteers, especially in association with corneal ulceration suggesting a role of SE. So far, it is unknown whether SE leads to tissue damage of the cornea by initiating an immune response or has direct toxic effects.

Original languageEnglish
Pages (from-to)799-803
Number of pages5
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume67
Issue number6
DOIs
Publication statusPublished - 2012 Jun

Fingerprint

Keratoconjunctivitis
Enterotoxins
Allergic Conjunctivitis
Corneal Ulcer
Staphylococcus aureus
Superantigens
Healthy Volunteers
Lacrimal Apparatus
Eye Diseases
Poisons
Conjunctiva
Eczema
Eyelids
Atopic Dermatitis
Fluorescein
Cornea
Genes

Keywords

  • corneal
  • enterotoxin
  • keratoconjunctivitis
  • staphylococcus
  • ulcer

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

The role of Staphylococcal enterotoxin in atopic keratoconjunctivitis and corneal ulceration. / Fujishima, H.; Okada, N.; Dogru, M.; Baba, F.; Tomita, Masaru; Abe, J.; Matsumoto, K.; Saito, H.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 67, No. 6, 06.2012, p. 799-803.

Research output: Contribution to journalArticle

Fujishima, H. ; Okada, N. ; Dogru, M. ; Baba, F. ; Tomita, Masaru ; Abe, J. ; Matsumoto, K. ; Saito, H. / The role of Staphylococcal enterotoxin in atopic keratoconjunctivitis and corneal ulceration. In: Allergy: European Journal of Allergy and Clinical Immunology. 2012 ; Vol. 67, No. 6. pp. 799-803.
@article{487c52094ef2435b9c390946b70ad6ff,
title = "The role of Staphylococcal enterotoxin in atopic keratoconjunctivitis and corneal ulceration",
abstract = "Background Patients with atopic eczema frequently experience colonization with Staphylococcus aureus that is directly correlated with the eczema severity. We hypothesized that S. aureus-secreted enterotoxins (SE) are involved in the pathophysiology of atopic keratoconjunctivitis (AKC). Methods A total of 45 subjects (18 with AKC, nine vernal keratoconjunctivitis (VKC), eight seasonal allergic conjunctivitis (SAC), and ten healthy volunteers) were enrolled. Slit lamp examinations, including fluorescein staining, were performed. Scraped samples were collected from the upper tarsal conjunctiva, lower conjunctival sacs, and the skin around the eyelid margins. Superantigen (SAg) genes were detected using polymerase chain reaction (PCR). Results Among 45 cases, S. aureus was detected significantly more in AKC patients than VKC patients (P = 0.026), SAC patients (P = 0.0003), and healthy volunteers (P = 0.0001). SAg genes were detected in 11 patients. SEB (2/11), SEG (8/11), and SEI (8/11) were detected, but no other SE. There was a significant difference in SE detection between AKC and SAC patients (P = 0.03). In severe types of ocular allergic disease such as AKC and VKC (N = 27), SE was detected in six of ten patients with corneal ulcers and two of 17 patients without corneal ulcers. SE was detected in significantly more patients with corneal ulcers (P = 0.025). Conclusions In patients with AKC, S. aureus and SE were detected more frequently compared with other patients and healthy volunteers, especially in association with corneal ulceration suggesting a role of SE. So far, it is unknown whether SE leads to tissue damage of the cornea by initiating an immune response or has direct toxic effects.",
keywords = "corneal, enterotoxin, keratoconjunctivitis, staphylococcus, ulcer",
author = "H. Fujishima and N. Okada and M. Dogru and F. Baba and Masaru Tomita and J. Abe and K. Matsumoto and H. Saito",
year = "2012",
month = "6",
doi = "10.1111/j.1398-9995.2012.02818.x",
language = "English",
volume = "67",
pages = "799--803",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
issn = "0105-4538",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - The role of Staphylococcal enterotoxin in atopic keratoconjunctivitis and corneal ulceration

AU - Fujishima, H.

AU - Okada, N.

AU - Dogru, M.

AU - Baba, F.

AU - Tomita, Masaru

AU - Abe, J.

AU - Matsumoto, K.

AU - Saito, H.

PY - 2012/6

Y1 - 2012/6

N2 - Background Patients with atopic eczema frequently experience colonization with Staphylococcus aureus that is directly correlated with the eczema severity. We hypothesized that S. aureus-secreted enterotoxins (SE) are involved in the pathophysiology of atopic keratoconjunctivitis (AKC). Methods A total of 45 subjects (18 with AKC, nine vernal keratoconjunctivitis (VKC), eight seasonal allergic conjunctivitis (SAC), and ten healthy volunteers) were enrolled. Slit lamp examinations, including fluorescein staining, were performed. Scraped samples were collected from the upper tarsal conjunctiva, lower conjunctival sacs, and the skin around the eyelid margins. Superantigen (SAg) genes were detected using polymerase chain reaction (PCR). Results Among 45 cases, S. aureus was detected significantly more in AKC patients than VKC patients (P = 0.026), SAC patients (P = 0.0003), and healthy volunteers (P = 0.0001). SAg genes were detected in 11 patients. SEB (2/11), SEG (8/11), and SEI (8/11) were detected, but no other SE. There was a significant difference in SE detection between AKC and SAC patients (P = 0.03). In severe types of ocular allergic disease such as AKC and VKC (N = 27), SE was detected in six of ten patients with corneal ulcers and two of 17 patients without corneal ulcers. SE was detected in significantly more patients with corneal ulcers (P = 0.025). Conclusions In patients with AKC, S. aureus and SE were detected more frequently compared with other patients and healthy volunteers, especially in association with corneal ulceration suggesting a role of SE. So far, it is unknown whether SE leads to tissue damage of the cornea by initiating an immune response or has direct toxic effects.

AB - Background Patients with atopic eczema frequently experience colonization with Staphylococcus aureus that is directly correlated with the eczema severity. We hypothesized that S. aureus-secreted enterotoxins (SE) are involved in the pathophysiology of atopic keratoconjunctivitis (AKC). Methods A total of 45 subjects (18 with AKC, nine vernal keratoconjunctivitis (VKC), eight seasonal allergic conjunctivitis (SAC), and ten healthy volunteers) were enrolled. Slit lamp examinations, including fluorescein staining, were performed. Scraped samples were collected from the upper tarsal conjunctiva, lower conjunctival sacs, and the skin around the eyelid margins. Superantigen (SAg) genes were detected using polymerase chain reaction (PCR). Results Among 45 cases, S. aureus was detected significantly more in AKC patients than VKC patients (P = 0.026), SAC patients (P = 0.0003), and healthy volunteers (P = 0.0001). SAg genes were detected in 11 patients. SEB (2/11), SEG (8/11), and SEI (8/11) were detected, but no other SE. There was a significant difference in SE detection between AKC and SAC patients (P = 0.03). In severe types of ocular allergic disease such as AKC and VKC (N = 27), SE was detected in six of ten patients with corneal ulcers and two of 17 patients without corneal ulcers. SE was detected in significantly more patients with corneal ulcers (P = 0.025). Conclusions In patients with AKC, S. aureus and SE were detected more frequently compared with other patients and healthy volunteers, especially in association with corneal ulceration suggesting a role of SE. So far, it is unknown whether SE leads to tissue damage of the cornea by initiating an immune response or has direct toxic effects.

KW - corneal

KW - enterotoxin

KW - keratoconjunctivitis

KW - staphylococcus

KW - ulcer

UR - http://www.scopus.com/inward/record.url?scp=84861098374&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861098374&partnerID=8YFLogxK

U2 - 10.1111/j.1398-9995.2012.02818.x

DO - 10.1111/j.1398-9995.2012.02818.x

M3 - Article

C2 - 22486642

AN - SCOPUS:84861098374

VL - 67

SP - 799

EP - 803

JO - Allergy: European Journal of Allergy and Clinical Immunology

JF - Allergy: European Journal of Allergy and Clinical Immunology

SN - 0105-4538

IS - 6

ER -