The role of stromal cells in the expression of interstitial collagenase (matrix metalloproteinase-1) in the invasion of gastric cancer

Yoshihiko Sakurai, Yoshihide Otani, Kaori Kameyama, Naoki Igarashi, Tetsuro Kubota, Koichiro Kumai, Masaki Kitajima

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background and Objectives: In one of the steps of tumor invasion and metastasis, tumor cells must invade surrounding tissues and degrade the components of the basement membranes. Interstitial collagenase (matrix metalloproteinase-1: MMP-1) has been also investigated in relation to cancer invasion and metastasis. Methods: We investigated the formation and mechanism of MMP-1 expression using gastric cancer cell lines and gastric fibroblasts derived from normal gastric mucosa by ELISA and immunohistochemistry. Results: Production of MMP-1 protein in gastric fibroblasts was stimulated significantly by adding the conditioned medium of MKN-74. Localization of MMP-1 protein in the xenografted gastric cancer cell lines was heterogeneous according to different cell lines. Conclusions: These results suggested that the production of MMP-1 protein in tumor invasion was regulated by interaction between stromal cells, particularly fibroblasts, and tumor cells.

Original languageEnglish
Pages (from-to)168-172
Number of pages5
JournalJournal of Surgical Oncology
Volume66
Issue number3
DOIs
Publication statusPublished - 1997 Nov

Fingerprint

Matrix Metalloproteinase 1
Stromal Cells
Matrix Metalloproteinases
Stomach Neoplasms
Fibroblasts
Neoplasms
Cell Line
Stomach
Neoplasm Metastasis
Proteins
Conditioned Culture Medium
Gastric Mucosa
Basement Membrane
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry

Keywords

  • Collagenase
  • Fibroblast
  • Gastric cancer
  • Matrix metalloproteinase-1
  • Stromal cell

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

The role of stromal cells in the expression of interstitial collagenase (matrix metalloproteinase-1) in the invasion of gastric cancer. / Sakurai, Yoshihiko; Otani, Yoshihide; Kameyama, Kaori; Igarashi, Naoki; Kubota, Tetsuro; Kumai, Koichiro; Kitajima, Masaki.

In: Journal of Surgical Oncology, Vol. 66, No. 3, 11.1997, p. 168-172.

Research output: Contribution to journalArticle

Sakurai, Yoshihiko ; Otani, Yoshihide ; Kameyama, Kaori ; Igarashi, Naoki ; Kubota, Tetsuro ; Kumai, Koichiro ; Kitajima, Masaki. / The role of stromal cells in the expression of interstitial collagenase (matrix metalloproteinase-1) in the invasion of gastric cancer. In: Journal of Surgical Oncology. 1997 ; Vol. 66, No. 3. pp. 168-172.
@article{409ea697ff4a4ba1be843f5cf7d8c77c,
title = "The role of stromal cells in the expression of interstitial collagenase (matrix metalloproteinase-1) in the invasion of gastric cancer",
abstract = "Background and Objectives: In one of the steps of tumor invasion and metastasis, tumor cells must invade surrounding tissues and degrade the components of the basement membranes. Interstitial collagenase (matrix metalloproteinase-1: MMP-1) has been also investigated in relation to cancer invasion and metastasis. Methods: We investigated the formation and mechanism of MMP-1 expression using gastric cancer cell lines and gastric fibroblasts derived from normal gastric mucosa by ELISA and immunohistochemistry. Results: Production of MMP-1 protein in gastric fibroblasts was stimulated significantly by adding the conditioned medium of MKN-74. Localization of MMP-1 protein in the xenografted gastric cancer cell lines was heterogeneous according to different cell lines. Conclusions: These results suggested that the production of MMP-1 protein in tumor invasion was regulated by interaction between stromal cells, particularly fibroblasts, and tumor cells.",
keywords = "Collagenase, Fibroblast, Gastric cancer, Matrix metalloproteinase-1, Stromal cell",
author = "Yoshihiko Sakurai and Yoshihide Otani and Kaori Kameyama and Naoki Igarashi and Tetsuro Kubota and Koichiro Kumai and Masaki Kitajima",
year = "1997",
month = "11",
doi = "10.1002/(SICI)1096-9098(199711)66:3<168::AID-JSO3>3.0.CO;2-A",
language = "English",
volume = "66",
pages = "168--172",
journal = "Journal of Surgical Oncology",
issn = "0022-4790",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - The role of stromal cells in the expression of interstitial collagenase (matrix metalloproteinase-1) in the invasion of gastric cancer

AU - Sakurai, Yoshihiko

AU - Otani, Yoshihide

AU - Kameyama, Kaori

AU - Igarashi, Naoki

AU - Kubota, Tetsuro

AU - Kumai, Koichiro

AU - Kitajima, Masaki

PY - 1997/11

Y1 - 1997/11

N2 - Background and Objectives: In one of the steps of tumor invasion and metastasis, tumor cells must invade surrounding tissues and degrade the components of the basement membranes. Interstitial collagenase (matrix metalloproteinase-1: MMP-1) has been also investigated in relation to cancer invasion and metastasis. Methods: We investigated the formation and mechanism of MMP-1 expression using gastric cancer cell lines and gastric fibroblasts derived from normal gastric mucosa by ELISA and immunohistochemistry. Results: Production of MMP-1 protein in gastric fibroblasts was stimulated significantly by adding the conditioned medium of MKN-74. Localization of MMP-1 protein in the xenografted gastric cancer cell lines was heterogeneous according to different cell lines. Conclusions: These results suggested that the production of MMP-1 protein in tumor invasion was regulated by interaction between stromal cells, particularly fibroblasts, and tumor cells.

AB - Background and Objectives: In one of the steps of tumor invasion and metastasis, tumor cells must invade surrounding tissues and degrade the components of the basement membranes. Interstitial collagenase (matrix metalloproteinase-1: MMP-1) has been also investigated in relation to cancer invasion and metastasis. Methods: We investigated the formation and mechanism of MMP-1 expression using gastric cancer cell lines and gastric fibroblasts derived from normal gastric mucosa by ELISA and immunohistochemistry. Results: Production of MMP-1 protein in gastric fibroblasts was stimulated significantly by adding the conditioned medium of MKN-74. Localization of MMP-1 protein in the xenografted gastric cancer cell lines was heterogeneous according to different cell lines. Conclusions: These results suggested that the production of MMP-1 protein in tumor invasion was regulated by interaction between stromal cells, particularly fibroblasts, and tumor cells.

KW - Collagenase

KW - Fibroblast

KW - Gastric cancer

KW - Matrix metalloproteinase-1

KW - Stromal cell

UR - http://www.scopus.com/inward/record.url?scp=0030686597&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030686597&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-9098(199711)66:3<168::AID-JSO3>3.0.CO;2-A

DO - 10.1002/(SICI)1096-9098(199711)66:3<168::AID-JSO3>3.0.CO;2-A

M3 - Article

VL - 66

SP - 168

EP - 172

JO - Journal of Surgical Oncology

JF - Journal of Surgical Oncology

SN - 0022-4790

IS - 3

ER -