The role of stromal cells in the expression of interstitial collagenase (matrix metalloproteinase-1) in the invasion of gastric cancer

Yoshihiko Sakurai, Yoshihide Otani, Kaori Kameyama, Naoki Igarashi, Tetsuro Kubota, Koichiro Kumai, Masaki Kitajima

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11 Citations (Scopus)


Background and Objectives: In one of the steps of tumor invasion and metastasis, tumor cells must invade surrounding tissues and degrade the components of the basement membranes. Interstitial collagenase (matrix metalloproteinase-1: MMP-1) has been also investigated in relation to cancer invasion and metastasis. Methods: We investigated the formation and mechanism of MMP-1 expression using gastric cancer cell lines and gastric fibroblasts derived from normal gastric mucosa by ELISA and immunohistochemistry. Results: Production of MMP-1 protein in gastric fibroblasts was stimulated significantly by adding the conditioned medium of MKN-74. Localization of MMP-1 protein in the xenografted gastric cancer cell lines was heterogeneous according to different cell lines. Conclusions: These results suggested that the production of MMP-1 protein in tumor invasion was regulated by interaction between stromal cells, particularly fibroblasts, and tumor cells.

Original languageEnglish
Pages (from-to)168-172
Number of pages5
JournalJournal of Surgical Oncology
Issue number3
Publication statusPublished - 1997 Nov 1



  • Collagenase
  • Fibroblast
  • Gastric cancer
  • Matrix metalloproteinase-1
  • Stromal cell

ASJC Scopus subject areas

  • Surgery
  • Oncology

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