The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain

Naoto Ohtani, Natsumi Saito, Masaru Tomita, Mitsuhiro Itaya, Aya Itoh

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The SCO2299 gene from Streptomyces coelicolor encodes a single peptide consisting of 497 amino acid residues. Its N-terminal region shows high amino acid sequence similarity to RNase HI, whereas its C-terminal region bears similarity to the CobC protein, which is involved in the synthesis of cobalamin. The SCO2299 gene suppressed a temperature-sensitive growth defect of an Escherichia coli RNase H-deficient strain, and the recombinant SCO2299 protein cleaved an RNA strand of RNA-DNA hybrid in vitro. The N-terminal domain of the SCO2299 protein, when overproduced independently, exhibited RNase H activity at a similar level to the full length protein. On the other hand, the C-terminal domain showed no CobC-like activity but an acid phosphatase activity. The full length protein also exhibited acid phosphatase activity at almost the same level as the C-terminal domain alone. These results indicate that RNase H and acid phosphatase activities of the full length SCO2299 protein depend on its N-terminal and C-terminal domains, respectively. The physiological functions of the SCO2299 gene and the relation between RNase H and acid phosphatase remain to be determined. However, the bifunctional enzyme examined here is a novel style in the Type 1 RNase H family. Additionally, S. coelicolor is the first example of an organism whose genome contains three active RNase H genes.

Original languageEnglish
Pages (from-to)2828-2837
Number of pages10
JournalFEBS Journal
Volume272
Issue number11
DOIs
Publication statusPublished - 2005 Jun

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Streptomyces coelicolor
Ribonuclease H
Acid Phosphatase
Genes
Enzymes
Proteins
RNA
Amino Acids
Vitamin B 12
Recombinant Proteins
Escherichia coli
2'-disulfonic acid 1-amino-8-nephthol-2
Amino Acid Sequence
Genome
Defects
Peptides
Temperature
DNA
Growth

ASJC Scopus subject areas

  • Biochemistry

Cite this

The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain. / Ohtani, Naoto; Saito, Natsumi; Tomita, Masaru; Itaya, Mitsuhiro; Itoh, Aya.

In: FEBS Journal, Vol. 272, No. 11, 06.2005, p. 2828-2837.

Research output: Contribution to journalArticle

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