The selective control of glycolysis, gluconeogenesis and glycogenesis by temporal insulin patterns

Rei Noguchi, Hiroyuki Kubota, Katsuyuki Yugi, Yu Toyoshima, Yasunori Komori, Tomoyoshi Soga, Shinya Kuroda

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Insulin governs systemic glucose metabolism, including glycolysis, gluconeogenesis and glycogenesis, through temporal change and absolute concentration. However, how insulin-signalling pathway selectively regulates glycolysis, gluconeogenesis and glycogenesis remains to be elucidated. To address this issue, we experimentally measured metabolites in glucose metabolism in response to insulin. Step stimulation of insulin induced transient response of glycolysis and glycogenesis, and sustained response of gluconeogenesis and extracellular glucose concentration (GLC<inf>ex</inf>). Based on the experimental results, we constructed a simple computational model that characterises response of insulin-signalling-dependent glucose metabolism. The model revealed that the network motifs of glycolysis and glycogenesis pathways constitute a feedforward (FF) with substrate depletion and incoherent feedforward loop (iFFL), respectively, enabling glycolysis and glycogenesis responsive to temporal changes of insulin rather than its absolute concentration. In contrast, the network motifs of gluconeogenesis pathway constituted a FF inhibition, enabling gluconeogenesis responsive to absolute concentration of insulin regardless of its temporal patterns. GLC<inf>ex</inf> was regulated by gluconeogenesis and glycolysis. These results demonstrate the selective control mechanism of glucose metabolism by temporal patterns of insulin.

Original languageEnglish
Article number664
JournalMolecular Systems Biology
Volume9
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

glycogenesis
Glycolysis
Gluconeogenesis
gluconeogenesis
Insulin
glycolysis
insulin
Glucose
Metabolism
Feedforward
glucose
metabolism
Pathway
Signaling Pathways
Transient Response
Depletion
Metabolites
Transient analysis
Computational Model
Substrate

Keywords

  • computational model
  • glucose metabolism
  • insulin
  • network motif
  • temporal coding

ASJC Scopus subject areas

  • Applied Mathematics
  • Information Systems
  • Computational Theory and Mathematics
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

The selective control of glycolysis, gluconeogenesis and glycogenesis by temporal insulin patterns. / Noguchi, Rei; Kubota, Hiroyuki; Yugi, Katsuyuki; Toyoshima, Yu; Komori, Yasunori; Soga, Tomoyoshi; Kuroda, Shinya.

In: Molecular Systems Biology, Vol. 9, No. 1, 664, 2013.

Research output: Contribution to journalArticle

Noguchi, Rei ; Kubota, Hiroyuki ; Yugi, Katsuyuki ; Toyoshima, Yu ; Komori, Yasunori ; Soga, Tomoyoshi ; Kuroda, Shinya. / The selective control of glycolysis, gluconeogenesis and glycogenesis by temporal insulin patterns. In: Molecular Systems Biology. 2013 ; Vol. 9, No. 1.
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