The serum factor from patients with ulcerative colitis that induces T cell proliferation in the mouse thymus is interleukin-7

Mamoru Watanabe, Noriaki Watanabe, Yasushi Iwao, Haruhiko Ogata, Takanori Kanai, Yoshitaka Ueno, Masaharu Tsuchiya, Hiromasa Ishii, Sadakazu Aiso, Sonoko Habu, Toshifumi Hibi

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The disturbance of immune regulatory T cells is related to the pathogenesis of ulcerative colitis. Here we demonstrated and characterized the serum factor from ulcerative colitis patients that induced proliferation of intrathymic T cells. The factor isolated from the patient sera by a combination of gel filtration and anion-exchange chromatography induced proliferation of CD4+CD8- intrathymic T cells in the organ-cultured embryonic mouse thymus. Purification and amino acid sequence analysis of the serum factor demonstrated that the N-terminal 12 sequence was homologous to that of interleukin-7. SDS-PAGE and Western blot confirmed that purified serum factor was interleukin-7. Enzyme immunoassay demonstrated that the serum interleukin-7 concentration was significantly increased in the patients. PCR and Southern blot hybridization demonstrated that interleukin- 7 mRNA expression was increased in the thymus tissues from patients but decreased in the colonic mucosa. Since interleukin-7 is a crucial cytokine for proliferation and differentiation of T cells in the thymus, the present study indicates that interleukin-7 may contribute to the disturbance of immune regulatory T cells in ulcerative colitis.

Original languageEnglish
Pages (from-to)282-292
Number of pages11
JournalJournal of Clinical Immunology
Volume17
Issue number4
DOIs
Publication statusPublished - 1997 Sep 4

Keywords

  • Interleukin-7
  • T cell
  • Thymus
  • Ulcerative colitis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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