The Significance of the Trp 64 Arg mutation of the β3-Adrenergic receptor gene in impaired glucose tolerance, non-isulin-dependent diabetes mellitus, and insulin resistance in Japanese subjects

Nobuyuki Azuma, Yasunao Yoshimasa, Haruo Nishimura, Yuji Yamamoto, Hiroaki Masuzaki, Junko Suga, Michika Shigemoto, Naoki Matsuoka, Tokuji Tanaka, Noriko Satoh, Toshio Igaki, Yoshihiro Miyamoto, Hiroshi Itoh, Takaaki Yoshimasa, Kiminori Hosoda, Shigeo Nishi, Kazuwa Nakao

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

It has been reported that the trp 64 Arg mutation of the human β3- adrenergic receptor β3-AR) gene is related to an earlier age of onset of non-insulin-dependent diabetes mellitus (NIDDM) and features of insulin resistance and weight gain in morbidly obese patients. However, such findings have not been consistent in varying ethnic populations. In the present study, we investigated the frequency of the Trp 64 Arg mutation of the human Ab3- AR gene in Japanese control subject (n = 253) and in NIDDM (n = 314) and impaired glucose tolerance (IGT) patients (N = 100). We compared the frequency of the mutation with the body-mass index (BMI) in the these groups and with the metabolic clearance rate (MCR) of glucose in the NIDDM patients. A Trp 64 Arg mutation was observed in 36.7%, 31.6%, and 37.0% of the control, NIDDM, and IGT subject, respectively. The frequency of the homozygotes for the mutation was 4.3%, 4.8/5 and 3.0%, respectively. Neither the genotype frequency (Trp/Arg, Arg/Arg) nor the frequency of the mutated allele was significantly different among the three groups. The BMI of the subjects with the mutation was not significantly higher than that of the subjects without the mutation in each group. Furthermore, the allele frequency (A) was not different among the subjects with different BMIs (BMI < 22.0, 22.0 ≤ BMI ≤ 26.4, BMI > 26.4) in each group. In a separate group of NIDDM patients, the MCR of the subjects with intermediate BMIs (22.0 ≤ BMI ≤ 26.4) with the mutation tended to be lower than that of those without the mutation. In addition, the MCR of the subjects with the mutation in this group was significantly lower compared with that of those with a BMI less than 22. These results indicate that the Trp 64 Arg mutation of the β3-AR gene may not contribute to the development of NIDDM or be a determinant of obesity in the Japanese population. However, the mutation may contribute to insulin resistance in NIDDM patients with an intermediate BMI.

Original languageEnglish
Pages (from-to)456-460
Number of pages5
JournalMetabolism: Clinical and Experimental
Volume47
Issue number4
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Glucose Intolerance
Adrenergic Receptors
Insulin Resistance
Diabetes Mellitus
Type 2 Diabetes Mellitus
Mutation
Body Mass Index
Genes
Metabolic Clearance Rate
Mutation Rate
Gene Frequency
Homozygote
Age of Onset
Population
Weight Gain
Obesity
Genotype
Glucose

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

The Significance of the Trp 64 Arg mutation of the β3-Adrenergic receptor gene in impaired glucose tolerance, non-isulin-dependent diabetes mellitus, and insulin resistance in Japanese subjects. / Azuma, Nobuyuki; Yoshimasa, Yasunao; Nishimura, Haruo; Yamamoto, Yuji; Masuzaki, Hiroaki; Suga, Junko; Shigemoto, Michika; Matsuoka, Naoki; Tanaka, Tokuji; Satoh, Noriko; Igaki, Toshio; Miyamoto, Yoshihiro; Itoh, Hiroshi; Yoshimasa, Takaaki; Hosoda, Kiminori; Nishi, Shigeo; Nakao, Kazuwa.

In: Metabolism: Clinical and Experimental, Vol. 47, No. 4, 1998, p. 456-460.

Research output: Contribution to journalArticle

Azuma, N, Yoshimasa, Y, Nishimura, H, Yamamoto, Y, Masuzaki, H, Suga, J, Shigemoto, M, Matsuoka, N, Tanaka, T, Satoh, N, Igaki, T, Miyamoto, Y, Itoh, H, Yoshimasa, T, Hosoda, K, Nishi, S & Nakao, K 1998, 'The Significance of the Trp 64 Arg mutation of the β3-Adrenergic receptor gene in impaired glucose tolerance, non-isulin-dependent diabetes mellitus, and insulin resistance in Japanese subjects', Metabolism: Clinical and Experimental, vol. 47, no. 4, pp. 456-460. https://doi.org/10.1016/S0026-0495(98)90059-2
Azuma, Nobuyuki ; Yoshimasa, Yasunao ; Nishimura, Haruo ; Yamamoto, Yuji ; Masuzaki, Hiroaki ; Suga, Junko ; Shigemoto, Michika ; Matsuoka, Naoki ; Tanaka, Tokuji ; Satoh, Noriko ; Igaki, Toshio ; Miyamoto, Yoshihiro ; Itoh, Hiroshi ; Yoshimasa, Takaaki ; Hosoda, Kiminori ; Nishi, Shigeo ; Nakao, Kazuwa. / The Significance of the Trp 64 Arg mutation of the β3-Adrenergic receptor gene in impaired glucose tolerance, non-isulin-dependent diabetes mellitus, and insulin resistance in Japanese subjects. In: Metabolism: Clinical and Experimental. 1998 ; Vol. 47, No. 4. pp. 456-460.
@article{879e83f15da34d48be4148f9f394e2bd,
title = "The Significance of the Trp 64 Arg mutation of the β3-Adrenergic receptor gene in impaired glucose tolerance, non-isulin-dependent diabetes mellitus, and insulin resistance in Japanese subjects",
abstract = "It has been reported that the trp 64 Arg mutation of the human β3- adrenergic receptor β3-AR) gene is related to an earlier age of onset of non-insulin-dependent diabetes mellitus (NIDDM) and features of insulin resistance and weight gain in morbidly obese patients. However, such findings have not been consistent in varying ethnic populations. In the present study, we investigated the frequency of the Trp 64 Arg mutation of the human Ab3- AR gene in Japanese control subject (n = 253) and in NIDDM (n = 314) and impaired glucose tolerance (IGT) patients (N = 100). We compared the frequency of the mutation with the body-mass index (BMI) in the these groups and with the metabolic clearance rate (MCR) of glucose in the NIDDM patients. A Trp 64 Arg mutation was observed in 36.7{\%}, 31.6{\%}, and 37.0{\%} of the control, NIDDM, and IGT subject, respectively. The frequency of the homozygotes for the mutation was 4.3{\%}, 4.8/5 and 3.0{\%}, respectively. Neither the genotype frequency (Trp/Arg, Arg/Arg) nor the frequency of the mutated allele was significantly different among the three groups. The BMI of the subjects with the mutation was not significantly higher than that of the subjects without the mutation in each group. Furthermore, the allele frequency (A) was not different among the subjects with different BMIs (BMI < 22.0, 22.0 ≤ BMI ≤ 26.4, BMI > 26.4) in each group. In a separate group of NIDDM patients, the MCR of the subjects with intermediate BMIs (22.0 ≤ BMI ≤ 26.4) with the mutation tended to be lower than that of those without the mutation. In addition, the MCR of the subjects with the mutation in this group was significantly lower compared with that of those with a BMI less than 22. These results indicate that the Trp 64 Arg mutation of the β3-AR gene may not contribute to the development of NIDDM or be a determinant of obesity in the Japanese population. However, the mutation may contribute to insulin resistance in NIDDM patients with an intermediate BMI.",
author = "Nobuyuki Azuma and Yasunao Yoshimasa and Haruo Nishimura and Yuji Yamamoto and Hiroaki Masuzaki and Junko Suga and Michika Shigemoto and Naoki Matsuoka and Tokuji Tanaka and Noriko Satoh and Toshio Igaki and Yoshihiro Miyamoto and Hiroshi Itoh and Takaaki Yoshimasa and Kiminori Hosoda and Shigeo Nishi and Kazuwa Nakao",
year = "1998",
doi = "10.1016/S0026-0495(98)90059-2",
language = "English",
volume = "47",
pages = "456--460",
journal = "Metabolism: Clinical and Experimental",
issn = "0026-0495",
publisher = "W.B. Saunders Ltd",
number = "4",

}

TY - JOUR

T1 - The Significance of the Trp 64 Arg mutation of the β3-Adrenergic receptor gene in impaired glucose tolerance, non-isulin-dependent diabetes mellitus, and insulin resistance in Japanese subjects

AU - Azuma, Nobuyuki

AU - Yoshimasa, Yasunao

AU - Nishimura, Haruo

AU - Yamamoto, Yuji

AU - Masuzaki, Hiroaki

AU - Suga, Junko

AU - Shigemoto, Michika

AU - Matsuoka, Naoki

AU - Tanaka, Tokuji

AU - Satoh, Noriko

AU - Igaki, Toshio

AU - Miyamoto, Yoshihiro

AU - Itoh, Hiroshi

AU - Yoshimasa, Takaaki

AU - Hosoda, Kiminori

AU - Nishi, Shigeo

AU - Nakao, Kazuwa

PY - 1998

Y1 - 1998

N2 - It has been reported that the trp 64 Arg mutation of the human β3- adrenergic receptor β3-AR) gene is related to an earlier age of onset of non-insulin-dependent diabetes mellitus (NIDDM) and features of insulin resistance and weight gain in morbidly obese patients. However, such findings have not been consistent in varying ethnic populations. In the present study, we investigated the frequency of the Trp 64 Arg mutation of the human Ab3- AR gene in Japanese control subject (n = 253) and in NIDDM (n = 314) and impaired glucose tolerance (IGT) patients (N = 100). We compared the frequency of the mutation with the body-mass index (BMI) in the these groups and with the metabolic clearance rate (MCR) of glucose in the NIDDM patients. A Trp 64 Arg mutation was observed in 36.7%, 31.6%, and 37.0% of the control, NIDDM, and IGT subject, respectively. The frequency of the homozygotes for the mutation was 4.3%, 4.8/5 and 3.0%, respectively. Neither the genotype frequency (Trp/Arg, Arg/Arg) nor the frequency of the mutated allele was significantly different among the three groups. The BMI of the subjects with the mutation was not significantly higher than that of the subjects without the mutation in each group. Furthermore, the allele frequency (A) was not different among the subjects with different BMIs (BMI < 22.0, 22.0 ≤ BMI ≤ 26.4, BMI > 26.4) in each group. In a separate group of NIDDM patients, the MCR of the subjects with intermediate BMIs (22.0 ≤ BMI ≤ 26.4) with the mutation tended to be lower than that of those without the mutation. In addition, the MCR of the subjects with the mutation in this group was significantly lower compared with that of those with a BMI less than 22. These results indicate that the Trp 64 Arg mutation of the β3-AR gene may not contribute to the development of NIDDM or be a determinant of obesity in the Japanese population. However, the mutation may contribute to insulin resistance in NIDDM patients with an intermediate BMI.

AB - It has been reported that the trp 64 Arg mutation of the human β3- adrenergic receptor β3-AR) gene is related to an earlier age of onset of non-insulin-dependent diabetes mellitus (NIDDM) and features of insulin resistance and weight gain in morbidly obese patients. However, such findings have not been consistent in varying ethnic populations. In the present study, we investigated the frequency of the Trp 64 Arg mutation of the human Ab3- AR gene in Japanese control subject (n = 253) and in NIDDM (n = 314) and impaired glucose tolerance (IGT) patients (N = 100). We compared the frequency of the mutation with the body-mass index (BMI) in the these groups and with the metabolic clearance rate (MCR) of glucose in the NIDDM patients. A Trp 64 Arg mutation was observed in 36.7%, 31.6%, and 37.0% of the control, NIDDM, and IGT subject, respectively. The frequency of the homozygotes for the mutation was 4.3%, 4.8/5 and 3.0%, respectively. Neither the genotype frequency (Trp/Arg, Arg/Arg) nor the frequency of the mutated allele was significantly different among the three groups. The BMI of the subjects with the mutation was not significantly higher than that of the subjects without the mutation in each group. Furthermore, the allele frequency (A) was not different among the subjects with different BMIs (BMI < 22.0, 22.0 ≤ BMI ≤ 26.4, BMI > 26.4) in each group. In a separate group of NIDDM patients, the MCR of the subjects with intermediate BMIs (22.0 ≤ BMI ≤ 26.4) with the mutation tended to be lower than that of those without the mutation. In addition, the MCR of the subjects with the mutation in this group was significantly lower compared with that of those with a BMI less than 22. These results indicate that the Trp 64 Arg mutation of the β3-AR gene may not contribute to the development of NIDDM or be a determinant of obesity in the Japanese population. However, the mutation may contribute to insulin resistance in NIDDM patients with an intermediate BMI.

UR - http://www.scopus.com/inward/record.url?scp=0013689086&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0013689086&partnerID=8YFLogxK

U2 - 10.1016/S0026-0495(98)90059-2

DO - 10.1016/S0026-0495(98)90059-2

M3 - Article

C2 - 9550545

AN - SCOPUS:0013689086

VL - 47

SP - 456

EP - 460

JO - Metabolism: Clinical and Experimental

JF - Metabolism: Clinical and Experimental

SN - 0026-0495

IS - 4

ER -