The significant cardiomyogenic potential of human umbilical cord blood-derived mesenchymal stem cells in vitro

Nobuhiro Nishiyama, Shunichiro Miyoshi, Naoko Hida, Taro Uyama, Kazuma Okamoto, Yukinori Ikegami, Kenji Miyado, Kaoru Segawa, Masanori Terai, Michiie Sakamoto, Satoshi Ogawa, Akihiro Umezawa

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Abstract

We tested the cardiomyogenic potential of the human umbilical cord blood-derived mesenchymal stem cells (UCBMSCs). Both the number and function of stem cells may be depressed in senile patients with severe coronary risk factors. Therefore, stem cells obtained from such patients may not function well. For this reason, UCBMSCs are potentially a new cell source for stem cell-based therapy, since such cells can be obtained from younger populations and are being routinely utilized for clinical patients. The human UCBMSCs (5 × 103 per cm2) were cocultured with fetal murine cardiomyocytes ([CM] 1 × 105 per cm2). On day 5 of cocultivation, approximately half of the green fluorescent protein (GFP)-labeled UCBMSCs contracted rhythmically and synchronously, suggesting the presence of electrical communication between the UCBMSCs. The fractional shortening of the contracted UCBMSCs was 6.5% ± 0.7% (n = 20). The UCBMSC-derived cardiomyocytes stained positive for cardiac troponin-I (clear striation +) and connexin 43 (diffuse dot-like staining at the margin of the cell) by the immunocytochemical method. Cardiac troponin-I positive cardiomyocytes accounted for 45% ± 3% of GFP-labeled UCBMSCs. The cardiomyocyte-specific long action potential duration (186 ± 12 milliseconds) was recorded with a glass microelectrode from the GFP-labeled UCBMSCs. CM were observed in UCBMSCs, which were cocultivated in the same dish with mouse cardiomyocytes separated by a collagen membrane. Cell fusion, therefore, was not a major cause of CM in the UCBMSCs. Approximately half of the human UCBMSCs were successfully transdifferentiated into cardiomyocytes in vitro. UCBMSCs can be a promising cellular source for cardiac stem cell-based therapy.

Original languageEnglish
Pages (from-to)2017-2024
Number of pages8
JournalStem Cells
Volume25
Issue number8
DOIs
Publication statusPublished - 2007 Aug

Fingerprint

Mesenchymal Stromal Cells
Fetal Blood
Cardiac Myocytes
Stem Cells
Green Fluorescent Proteins
Troponin I
Cell- and Tissue-Based Therapy
In Vitro Techniques
Connexin 43
Cell Fusion
Microelectrodes
Coculture Techniques
Action Potentials
Glass
Collagen
Communication
Staining and Labeling

Keywords

  • Action potentials
  • Cells
  • Heart failure
  • Physiology
  • Transplantation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Nishiyama, N., Miyoshi, S., Hida, N., Uyama, T., Okamoto, K., Ikegami, Y., ... Umezawa, A. (2007). The significant cardiomyogenic potential of human umbilical cord blood-derived mesenchymal stem cells in vitro. Stem Cells, 25(8), 2017-2024. https://doi.org/10.1634/stemcells.2006-0662

The significant cardiomyogenic potential of human umbilical cord blood-derived mesenchymal stem cells in vitro. / Nishiyama, Nobuhiro; Miyoshi, Shunichiro; Hida, Naoko; Uyama, Taro; Okamoto, Kazuma; Ikegami, Yukinori; Miyado, Kenji; Segawa, Kaoru; Terai, Masanori; Sakamoto, Michiie; Ogawa, Satoshi; Umezawa, Akihiro.

In: Stem Cells, Vol. 25, No. 8, 08.2007, p. 2017-2024.

Research output: Contribution to journalArticle

Nishiyama, N, Miyoshi, S, Hida, N, Uyama, T, Okamoto, K, Ikegami, Y, Miyado, K, Segawa, K, Terai, M, Sakamoto, M, Ogawa, S & Umezawa, A 2007, 'The significant cardiomyogenic potential of human umbilical cord blood-derived mesenchymal stem cells in vitro', Stem Cells, vol. 25, no. 8, pp. 2017-2024. https://doi.org/10.1634/stemcells.2006-0662
Nishiyama, Nobuhiro ; Miyoshi, Shunichiro ; Hida, Naoko ; Uyama, Taro ; Okamoto, Kazuma ; Ikegami, Yukinori ; Miyado, Kenji ; Segawa, Kaoru ; Terai, Masanori ; Sakamoto, Michiie ; Ogawa, Satoshi ; Umezawa, Akihiro. / The significant cardiomyogenic potential of human umbilical cord blood-derived mesenchymal stem cells in vitro. In: Stem Cells. 2007 ; Vol. 25, No. 8. pp. 2017-2024.
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