The SOCS Box of SOCS-1 Accelerates Ubiquitin-dependent Proteolysis of TEL-JAK2

Shintaro Kamizono, Toshikatsu Hanada, Hideo Yasukawa, Shigeru Minoguchi, Reiko Kato, Mayu Minoguchi, Kimihiko Hattori, Shigetsugu Hatakeyama, Masayoshi Yada, Sumiyo Morita, Toshio Kitamura, Hirohisa Kato, Kei Ichi Nakayama, Akihiko Yoshimura

Research output: Contribution to journalArticle

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Abstract

Fusion of the TEL gene on 12p13 to the JAK2 tyrosine kinase gene on 9p24 has been found in human leukemia. TEL-mediated oligomerization of JAK2 results in constitutive activation of the tyrosine kinase (JH1) domain and confers cytokine-independent proliferation on interleukin-3-dependent Ba/F3 cells. Forced expression of the JAK inhibitor gene SOCS1/JAB/SSI-1 induced apoptosis of TEL-JAK2-transformed Ba/F3 cells. This suppression of TEL-JAK2 activity was dependent on SOCS box-mediated proteasomal degradation of TEL-JAK2 rather than on kinase inhibition. Degradation of JAK2 depended on its phosphorylation and its high affinity binding with SOCS1 through the kinase inhibitory region and the SH2 domain. It has been demonstrated that von Hippel-Lindau disease (VHL) tumor-suppressor gene product possesses the SOCS box that forms a complex with Elongin B and C and Cullin-2, and it functions as a ubiquitin ligase. The SOCS box of SOCS1/JAB has also been shown to interact with Elongins; however, ubiquitin ligase activity has not been demonstrated. We found that the SOCS box interacted with Cullin-2 and promoted ubiquitination of TEL-JAK2. Furthermore, overexpression of dominant negative Cullin-2 suppressed SOCS1-dependent TEL-JAK2 degradation. Our study demonstrates the substrate-specific E3 ubiquitin-ligase-like activity of SOCS1 for activated JAK2 and may provide a novel strategy for the suppression of oncogenic tyrosine kinases.

Original languageEnglish
Pages (from-to)12530-12538
Number of pages9
JournalJournal of Biological Chemistry
Volume276
Issue number16
DOIs
Publication statusPublished - 2001 Apr 20
Externally publishedYes

Fingerprint

Cullin Proteins
Proteolysis
Ubiquitin
Protein-Tyrosine Kinases
Genes
Ligases
Phosphotransferases
Degradation
von Hippel-Lindau Disease
Ubiquitin-Protein Ligases
src Homology Domains
Interleukin-3
Gene Fusion
Ubiquitination
Tumor Suppressor Genes
Oligomerization
Phosphorylation
Leukemia
Apoptosis
Cytokines

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kamizono, S., Hanada, T., Yasukawa, H., Minoguchi, S., Kato, R., Minoguchi, M., ... Yoshimura, A. (2001). The SOCS Box of SOCS-1 Accelerates Ubiquitin-dependent Proteolysis of TEL-JAK2. Journal of Biological Chemistry, 276(16), 12530-12538. https://doi.org/10.1074/jbc.M010074200

The SOCS Box of SOCS-1 Accelerates Ubiquitin-dependent Proteolysis of TEL-JAK2. / Kamizono, Shintaro; Hanada, Toshikatsu; Yasukawa, Hideo; Minoguchi, Shigeru; Kato, Reiko; Minoguchi, Mayu; Hattori, Kimihiko; Hatakeyama, Shigetsugu; Yada, Masayoshi; Morita, Sumiyo; Kitamura, Toshio; Kato, Hirohisa; Nakayama, Kei Ichi; Yoshimura, Akihiko.

In: Journal of Biological Chemistry, Vol. 276, No. 16, 20.04.2001, p. 12530-12538.

Research output: Contribution to journalArticle

Kamizono, S, Hanada, T, Yasukawa, H, Minoguchi, S, Kato, R, Minoguchi, M, Hattori, K, Hatakeyama, S, Yada, M, Morita, S, Kitamura, T, Kato, H, Nakayama, KI & Yoshimura, A 2001, 'The SOCS Box of SOCS-1 Accelerates Ubiquitin-dependent Proteolysis of TEL-JAK2', Journal of Biological Chemistry, vol. 276, no. 16, pp. 12530-12538. https://doi.org/10.1074/jbc.M010074200
Kamizono S, Hanada T, Yasukawa H, Minoguchi S, Kato R, Minoguchi M et al. The SOCS Box of SOCS-1 Accelerates Ubiquitin-dependent Proteolysis of TEL-JAK2. Journal of Biological Chemistry. 2001 Apr 20;276(16):12530-12538. https://doi.org/10.1074/jbc.M010074200
Kamizono, Shintaro ; Hanada, Toshikatsu ; Yasukawa, Hideo ; Minoguchi, Shigeru ; Kato, Reiko ; Minoguchi, Mayu ; Hattori, Kimihiko ; Hatakeyama, Shigetsugu ; Yada, Masayoshi ; Morita, Sumiyo ; Kitamura, Toshio ; Kato, Hirohisa ; Nakayama, Kei Ichi ; Yoshimura, Akihiko. / The SOCS Box of SOCS-1 Accelerates Ubiquitin-dependent Proteolysis of TEL-JAK2. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 16. pp. 12530-12538.
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AU - Morita, Sumiyo

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