The structure of the human thyrotropin β-subunit gene

Tatsumi Keita, Hayashizaki Yoshihide, Hiraoka Yoshiki, Miyai Kiyoshi, Matsubara Kenichi

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We have determined and characterized the entire structure of the human thyrotropin β-subunit (TSHβ) gene. It is 4.5 kb in size and separated into three exons. Primer extension analysis showed one transcription start point, and S1 mapping analysis showed two blocks of polyadenylation sites in normal pituitaries. The same transcripts were observed in TSH-producing adenomas. The 5'-flanking region contains two 'TATA' boxes, two 'CAAT' boxes, and two sequences similar to the cyclic AMP-responsive elements.

Original languageEnglish
Pages (from-to)489-497
Number of pages9
JournalGene
Volume73
Issue number2
DOIs
Publication statusPublished - 1988 Dec 20

Keywords

  • TSH-producing adenoma
  • conserved nucleotide sequence
  • exon
  • gene regulatory region
  • genomic library
  • promoter region
  • recombinant DNA: phage λ vector
  • transcription start point

ASJC Scopus subject areas

  • Genetics

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