The structure of the human thyrotropin β-subunit gene

Tatsumi Keita; Hayashizaki Yoshihide; Hiraoka Yoshiki; Miyai Kiyoshi; Matsubara Kenichi

doi:10.1016/0378-1119(88)90513-6

The structure of the human thyrotropin β-subunit gene

Tatsumi Keita, Hayashizaki Yoshihide, Hiraoka Yoshiki, Miyai Kiyoshi, Matsubara Kenichi

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

We have determined and characterized the entire structure of the human thyrotropin β-subunit (TSHβ) gene. It is 4.5 kb in size and separated into three exons. Primer extension analysis showed one transcription start point, and S1 mapping analysis showed two blocks of polyadenylation sites in normal pituitaries. The same transcripts were observed in TSH-producing adenomas. The 5'-flanking region contains two 'TATA' boxes, two 'CAAT' boxes, and two sequences similar to the cyclic AMP-responsive elements.

Original language	English
Pages (from-to)	489-497
Number of pages	9
Journal	Gene
Volume	73
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(88)90513-6
Publication status	Published - 1988 Dec 20
Externally published	Yes

Keywords

TSH-producing adenoma
conserved nucleotide sequence
exon
gene regulatory region
genomic library
promoter region
recombinant DNA: phage λ vector
transcription start point

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(88)90513-6

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abstract = "We have determined and characterized the entire structure of the human thyrotropin β-subunit (TSHβ) gene. It is 4.5 kb in size and separated into three exons. Primer extension analysis showed one transcription start point, and S1 mapping analysis showed two blocks of polyadenylation sites in normal pituitaries. The same transcripts were observed in TSH-producing adenomas. The 5'-flanking region contains two 'TATA' boxes, two 'CAAT' boxes, and two sequences similar to the cyclic AMP-responsive elements.",

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AU - Keita, Tatsumi

AU - Yoshihide, Hayashizaki

AU - Yoshiki, Hiraoka

AU - Kiyoshi, Miyai

AU - Kenichi, Matsubara

PY - 1988/12/20

Y1 - 1988/12/20

N2 - We have determined and characterized the entire structure of the human thyrotropin β-subunit (TSHβ) gene. It is 4.5 kb in size and separated into three exons. Primer extension analysis showed one transcription start point, and S1 mapping analysis showed two blocks of polyadenylation sites in normal pituitaries. The same transcripts were observed in TSH-producing adenomas. The 5'-flanking region contains two 'TATA' boxes, two 'CAAT' boxes, and two sequences similar to the cyclic AMP-responsive elements.

AB - We have determined and characterized the entire structure of the human thyrotropin β-subunit (TSHβ) gene. It is 4.5 kb in size and separated into three exons. Primer extension analysis showed one transcription start point, and S1 mapping analysis showed two blocks of polyadenylation sites in normal pituitaries. The same transcripts were observed in TSH-producing adenomas. The 5'-flanking region contains two 'TATA' boxes, two 'CAAT' boxes, and two sequences similar to the cyclic AMP-responsive elements.

KW - TSH-producing adenoma

KW - conserved nucleotide sequence

KW - exon

KW - gene regulatory region

KW - genomic library

KW - promoter region

KW - recombinant DNA: phage λ vector

KW - transcription start point

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The structure of the human thyrotropin β-subunit gene

Abstract

Keywords

ASJC Scopus subject areas

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