The study of the characteristic of photocytotoxicity under high peak power pulsed irradiation with ATX-S10Na(II) in vitro

Sayaka Ohmori, Kensuke Masuda, Masatake Yoshida, Tsunenori Arai, Susumu Nakajima

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We studied hydrophilic photosensitizer ATX-S10Na(II) mediated photocytotoxicity against macrophage-like cell under pulsed irradiation. We found that photocytotoxicity suppression under high intensity irradiation was directly induced by a decrease in the Type-II photoreaction. We showed that this decrease was not attributable to absorption saturation with the high intensity irradiation. We found the cell lethality change from 70% to 13% with the pulse peak power density ranging from 0.29 MW/cm2 to 1.36 MW/cm 2, at the light dose of 20 J/cm2 and the pulse repetition rate at 40 Hz. To investigate the Type-II reaction, we measured the photobleaching, oxygen consumption and singlet oxygen luminescence of the photosensitizer solution. The transient absorption from the photosensitizer during the irradiation was measured with the pump-and-probe technique. We believe that the photocytotoxicity suppression induced by the high intensity irradiation might be useful for the treatment of depth-controlled photodynamic therapy without the wall damage of a hollow organ.

Original languageEnglish
Pages (from-to)54-61
Number of pages8
JournalLasers in Medical Science
Volume20
Issue number2
DOIs
Publication statusPublished - 2005 Sep

Fingerprint

Photosensitizing Agents
Photobleaching
Singlet Oxygen
Photochemotherapy
Luminescence
Oxygen Consumption
Heart Rate
Macrophages
Light
ATX S10Na(II)
In Vitro Techniques
Therapeutics

Keywords

  • High peak power pulsed irradiation
  • Photodynamic therapy
  • Singlet oxygen generation
  • Treatment depth-controlled PDT
  • Type-II reaction

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Surgery

Cite this

The study of the characteristic of photocytotoxicity under high peak power pulsed irradiation with ATX-S10Na(II) in vitro. / Ohmori, Sayaka; Masuda, Kensuke; Yoshida, Masatake; Arai, Tsunenori; Nakajima, Susumu.

In: Lasers in Medical Science, Vol. 20, No. 2, 09.2005, p. 54-61.

Research output: Contribution to journalArticle

Ohmori, Sayaka ; Masuda, Kensuke ; Yoshida, Masatake ; Arai, Tsunenori ; Nakajima, Susumu. / The study of the characteristic of photocytotoxicity under high peak power pulsed irradiation with ATX-S10Na(II) in vitro. In: Lasers in Medical Science. 2005 ; Vol. 20, No. 2. pp. 54-61.
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