The study of the characteristic of photocytotoxicity under high peak power pulsed irradiation with ATX-S10Na(II) in vitro

Sayaka Ohmori, Kensuke Masuda, Masatake Yoshida, Tsunenori Arai, Susumu Nakajima

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We studied hydrophilic photosensitizer ATX-S10Na(II) mediated photocytotoxicity against macrophage-like cell under pulsed irradiation. We found that photocytotoxicity suppression under high intensity irradiation was directly induced by a decrease in the Type-II photoreaction. We showed that this decrease was not attributable to absorption saturation with the high intensity irradiation. We found the cell lethality change from 70% to 13% with the pulse peak power density ranging from 0.29 MW/cm2 to 1.36 MW/cm 2, at the light dose of 20 J/cm2 and the pulse repetition rate at 40 Hz. To investigate the Type-II reaction, we measured the photobleaching, oxygen consumption and singlet oxygen luminescence of the photosensitizer solution. The transient absorption from the photosensitizer during the irradiation was measured with the pump-and-probe technique. We believe that the photocytotoxicity suppression induced by the high intensity irradiation might be useful for the treatment of depth-controlled photodynamic therapy without the wall damage of a hollow organ.

Original languageEnglish
Pages (from-to)54-61
Number of pages8
JournalLasers in Medical Science
Volume20
Issue number2
DOIs
Publication statusPublished - 2005 Sep 1

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Keywords

  • High peak power pulsed irradiation
  • Photodynamic therapy
  • Singlet oxygen generation
  • Treatment depth-controlled PDT
  • Type-II reaction

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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