The suppressive effect of anti-asialo GM1 antibody on low-dose streptozotocin-induced diabetes in CD-1 mice

T. Maruyama, K. Watanabe, T. Yanagawa, K. Kasatani, A. Kasuga, A. Shimada, I. Takei, Y. Suzuki, K. Katoaka, T. Saruta

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Abstract

To elucidate the role of natural killer (NK) cells in the pathogenesis of diabetes in streptozotocin-induced diabetes, we examined whether treatment with anti-asialo GM1 antibody prevents the occurrence of diabetes in CD-1 mouse model. Anti-asialo GM1 antibody was injected intraperitoneally 2-3 times a week starting three days before the first strepto-zotocin injection. In controls, rabbit immunoglobulin or saline was injected instead of anti-asialo GM1 antibody. Three of twelve anti-asialo GM1 antibody-treated mice developed diabetes, however eight of eight (100%) rabbit immunoglobulin injected mice and 20 of 23 saline-injected mice developed diabetes. The incidence of diabetes in the anti-asialo GM1 antibody-injected group was significantly higher than in the two control groups (p<0.01, p<0.01, respectively). The NK-cell activities of spleen cells from anti-asialo GM1 antibody-treated mice were significantly lower than in control mice. Flowcyto-metry analysis demonstrated that anti-asialo GM1 antibody-positive cells had disappeared from spleens of anti-asialo GM1 antibody-injected mice but no suppression of T-lymphocytes could be demonstrated. These results suggest that NK cells play a role in the pathogenesis of streptozotocin-induced diabetes in CD-1 mice.

Original languageEnglish
Pages (from-to)171-175
Number of pages5
JournalDiabetes Research
Volume16
Issue number4
Publication statusPublished - 1991 Dec 1

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Keywords

  • Anti-asialo GM1 antibody
  • CD-1 mouse
  • Diabetes
  • NK cell
  • Streptozotocin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Maruyama, T., Watanabe, K., Yanagawa, T., Kasatani, K., Kasuga, A., Shimada, A., Takei, I., Suzuki, Y., Katoaka, K., & Saruta, T. (1991). The suppressive effect of anti-asialo GM1 antibody on low-dose streptozotocin-induced diabetes in CD-1 mice. Diabetes Research, 16(4), 171-175.