The suppressive effect of anti-asialo GM1 antibody on low-dose streptozotocin-induced diabetes in CD-1 mice

T. Maruyama, Kenji Watanabe, T. Yanagawa, K. Kasatani, A. Kasuga, A. Shimada, I. Takei, Y. Suzuki, K. Katoaka, T. Saruta

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

To elucidate the role of natural killer (NK) cells in the pathogenesis of diabetes in streptozotocin-induced diabetes, we examined whether treatment with anti-asialo GM1 antibody prevents the occurrence of diabetes in CD-1 mouse model. Anti-asialo GM1 antibody was injected intraperitoneally 2-3 times a week starting three days before the first strepto-zotocin injection. In controls, rabbit immunoglobulin or saline was injected instead of anti-asialo GM1 antibody. Three of twelve anti-asialo GM1 antibody-treated mice developed diabetes, however eight of eight (100%) rabbit immunoglobulin injected mice and 20 of 23 saline-injected mice developed diabetes. The incidence of diabetes in the anti-asialo GM1 antibody-injected group was significantly higher than in the two control groups (p<0.01, p<0.01, respectively). The NK-cell activities of spleen cells from anti-asialo GM1 antibody-treated mice were significantly lower than in control mice. Flowcyto-metry analysis demonstrated that anti-asialo GM1 antibody-positive cells had disappeared from spleens of anti-asialo GM1 antibody-injected mice but no suppression of T-lymphocytes could be demonstrated. These results suggest that NK cells play a role in the pathogenesis of streptozotocin-induced diabetes in CD-1 mice.

Original languageEnglish
Pages (from-to)171-175
Number of pages5
JournalDiabetes Research
Volume16
Issue number4
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Experimental Diabetes Mellitus
Antibodies
Natural Killer Cells
Immunoglobulins
Spleen
Rabbits
asialo GM1 ganglioside
T-Lymphocytes
Control Groups
Injections
Incidence

Keywords

  • Anti-asialo GM1 antibody
  • CD-1 mouse
  • Diabetes
  • NK cell
  • Streptozotocin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Maruyama, T., Watanabe, K., Yanagawa, T., Kasatani, K., Kasuga, A., Shimada, A., ... Saruta, T. (1991). The suppressive effect of anti-asialo GM1 antibody on low-dose streptozotocin-induced diabetes in CD-1 mice. Diabetes Research, 16(4), 171-175.

The suppressive effect of anti-asialo GM1 antibody on low-dose streptozotocin-induced diabetes in CD-1 mice. / Maruyama, T.; Watanabe, Kenji; Yanagawa, T.; Kasatani, K.; Kasuga, A.; Shimada, A.; Takei, I.; Suzuki, Y.; Katoaka, K.; Saruta, T.

In: Diabetes Research, Vol. 16, No. 4, 1991, p. 171-175.

Research output: Contribution to journalArticle

Maruyama, T, Watanabe, K, Yanagawa, T, Kasatani, K, Kasuga, A, Shimada, A, Takei, I, Suzuki, Y, Katoaka, K & Saruta, T 1991, 'The suppressive effect of anti-asialo GM1 antibody on low-dose streptozotocin-induced diabetes in CD-1 mice', Diabetes Research, vol. 16, no. 4, pp. 171-175.
Maruyama T, Watanabe K, Yanagawa T, Kasatani K, Kasuga A, Shimada A et al. The suppressive effect of anti-asialo GM1 antibody on low-dose streptozotocin-induced diabetes in CD-1 mice. Diabetes Research. 1991;16(4):171-175.
Maruyama, T. ; Watanabe, Kenji ; Yanagawa, T. ; Kasatani, K. ; Kasuga, A. ; Shimada, A. ; Takei, I. ; Suzuki, Y. ; Katoaka, K. ; Saruta, T. / The suppressive effect of anti-asialo GM1 antibody on low-dose streptozotocin-induced diabetes in CD-1 mice. In: Diabetes Research. 1991 ; Vol. 16, No. 4. pp. 171-175.
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