The surface carbohydrates of the Echinococcus granulosus larva interact selectively with the rodent Kupffer cell receptor

Tsui Ling Hsu, Gerardo Lin, Akihiko Koizumi, Klaus Brehm, Noriyasu Hada, Po Kai Chuang, Chi Huey Wong, Shie Liang Hsieh, Alvaro Díaz

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19 Citations (Scopus)

Abstract

The larvae of the cestodes belonging to the genus Echinococcus dwell primarily in mammalian liver. They are protected by the laminated layer (LL), an acellular mucin-based structure. The glycans decorating these mucins constitute the overwhelming majority of molecules exposed by these larvae to their hosts. However, their decoding by host innate immunity has not been studied. Out of 36 mammalian innate receptors with carbohydrate-binding domains, expressed as Fc fusions, only the mouse Kupffer cell receptor (KCR; CLEC4F) bound significantly to the Echinococcus granulosus LL mucins. The receptor also bound the Echinococcus multilocularis LL. Out of several synthetic glycans representing Echinococcus LL structures, the KCR bound strongly in particular to those ending in Galα1-4Galβ1-3 or Galα1-4Galβ1-4GlcNAc, both characteristic LL carbohydrate motifs. LL carbohydrates may be optimized to interact with the KCR, expressed only in liver macrophages, cells known to contribute to the tolerogenic antigen presentation that is characteristic of this organ.

Original languageEnglish
Pages (from-to)55-59
Number of pages5
JournalMolecular and Biochemical Parasitology
Volume192
Issue number1-2
DOIs
Publication statusPublished - 2013 Nov 1

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Keywords

  • Carbohydrate
  • Echinococcus
  • Kupffer cell receptor
  • Kupffer cells
  • Laminated layer
  • Liver

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology

Cite this

Hsu, T. L., Lin, G., Koizumi, A., Brehm, K., Hada, N., Chuang, P. K., Wong, C. H., Hsieh, S. L., & Díaz, A. (2013). The surface carbohydrates of the Echinococcus granulosus larva interact selectively with the rodent Kupffer cell receptor. Molecular and Biochemical Parasitology, 192(1-2), 55-59. https://doi.org/10.1016/j.molbiopara.2013.12.001