The transcriptional repressor RP58 is crucial for cell-division patterning and neuronal survival in the developing cortex

Haruo Okado, Chiaki Ohtaka-Maruyama, Yoshinobu Sugitani, Yuko Fukuda, Reiko Ishida, Shinobu Hirai, Akiko Miwa, Akiyo Takahashi, Katsunori Aoki, Keiji Mochida, Osamu Suzuki, Takao Honda, Kazunori Nakajima, Masaharu Ogawa, Toshio Terashima, Junichiro Matsuda, Hitoshi Kawano, Masataka Kasai

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The neocortex and the hippocampus comprise several specific layers containing distinct neurons that originate from progenitors at specific development times, under the control of an adequate cell-division patterning mechanism. Although many molecules are known to regulate this cell-division patterning process, its details are not well understood. Here, we show that, in the developing cerebral cortex, the RP58 transcription repressor protein was expressed both in postmitotic glutamatergic projection neurons and in their progenitor cells, but not in GABAergic interneurons. Targeted deletion of the RP58 gene led to dysplasia of the neocortex and of the hippocampus, reduction of the number of mature cortical neurons, and defects of laminar organization, which reflect abnormal neuronal migration within the cortical plate. We demonstrate an impairment of the cell-division patterning during the late embryonic stage and an enhancement of apoptosis of the postmitotic neurons in the RP58-deficient cortex. These results suggest that RP58 controls cell division of progenitor cells and regulates the survival of postmitotic cortical neurons.

Original languageEnglish
Pages (from-to)140-151
Number of pages12
JournalDevelopmental Biology
Volume331
Issue number2
DOIs
Publication statusPublished - 2009 Jul 15

Fingerprint

Cell Division
Neurons
Neocortex
Cerebral Cortex
Hippocampus
Stem Cells
Repressor Proteins
Gene Deletion
Interneurons
Cell Survival
Apoptosis

Keywords

  • Apoptosis
  • Cell-cycle exit
  • Cerebral cortex
  • Progenitor cell
  • RP58
  • Transcriptional repressor

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Okado, H., Ohtaka-Maruyama, C., Sugitani, Y., Fukuda, Y., Ishida, R., Hirai, S., ... Kasai, M. (2009). The transcriptional repressor RP58 is crucial for cell-division patterning and neuronal survival in the developing cortex. Developmental Biology, 331(2), 140-151. https://doi.org/10.1016/j.ydbio.2009.04.030

The transcriptional repressor RP58 is crucial for cell-division patterning and neuronal survival in the developing cortex. / Okado, Haruo; Ohtaka-Maruyama, Chiaki; Sugitani, Yoshinobu; Fukuda, Yuko; Ishida, Reiko; Hirai, Shinobu; Miwa, Akiko; Takahashi, Akiyo; Aoki, Katsunori; Mochida, Keiji; Suzuki, Osamu; Honda, Takao; Nakajima, Kazunori; Ogawa, Masaharu; Terashima, Toshio; Matsuda, Junichiro; Kawano, Hitoshi; Kasai, Masataka.

In: Developmental Biology, Vol. 331, No. 2, 15.07.2009, p. 140-151.

Research output: Contribution to journalArticle

Okado, H, Ohtaka-Maruyama, C, Sugitani, Y, Fukuda, Y, Ishida, R, Hirai, S, Miwa, A, Takahashi, A, Aoki, K, Mochida, K, Suzuki, O, Honda, T, Nakajima, K, Ogawa, M, Terashima, T, Matsuda, J, Kawano, H & Kasai, M 2009, 'The transcriptional repressor RP58 is crucial for cell-division patterning and neuronal survival in the developing cortex', Developmental Biology, vol. 331, no. 2, pp. 140-151. https://doi.org/10.1016/j.ydbio.2009.04.030
Okado, Haruo ; Ohtaka-Maruyama, Chiaki ; Sugitani, Yoshinobu ; Fukuda, Yuko ; Ishida, Reiko ; Hirai, Shinobu ; Miwa, Akiko ; Takahashi, Akiyo ; Aoki, Katsunori ; Mochida, Keiji ; Suzuki, Osamu ; Honda, Takao ; Nakajima, Kazunori ; Ogawa, Masaharu ; Terashima, Toshio ; Matsuda, Junichiro ; Kawano, Hitoshi ; Kasai, Masataka. / The transcriptional repressor RP58 is crucial for cell-division patterning and neuronal survival in the developing cortex. In: Developmental Biology. 2009 ; Vol. 331, No. 2. pp. 140-151.
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