The use of a temperature-responsive column for the direct analysis of drugs in serum by two-dimensional heart-cutting liquid chromatography

Toshiyasu Mikuma, Ryo Uchida, Mizuki Kajiya, Yuki Hiruta, Hideko Kanazawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A novel pretreatment method, which was performed using a two-dimensional high-performance liquid chromatography (2D-HPLC) system, was proposed for the direct analysis of drugs in human serum. A temperature-responsive column was used as a pretreatment column. The stationary phase of the temperature-responsive column exhibits temperature-regulated hydrophilic/hydrophobic characteristics. Controlling the ionic strength of the eluent enables human serum albumin (HSA) to pass through the column without retention. When serum samples containing barbiturates or benzodiazepines were injected into the temperature-responsive column using 10 mM of ammonium acetate (pH 6.5) as the mobile phase and in the temperature range of 10–40 °C, HSA was eluted from the column near the dead time, followed by the individual drugs. When the column temperature was changed, the retention times of the drugs were altered owing to surface property changes within the pretreatment column. These closely eluted compounds were subsequently introduced into the analytical column using a column-switching valve, with a minimal gap time to avoid foreign substance contamination. This new 2D-HPLC method afforded high-quality chromatograms of multiple drugs without unwanted peaks from foreign substances. The present technique could be an attractive choice in selecting the analytical method for drug analysis.

Original languageEnglish
Pages (from-to)1059-1065
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume409
Issue number4
DOIs
Publication statusPublished - 2017 Feb 1

Fingerprint

Liquid chromatography
Liquid Chromatography
Temperature
Serum
Pharmaceutical Preparations
High Pressure Liquid Chromatography
Serum Albumin
Barbiturates
Surface Properties
High performance liquid chromatography
Ionic strength
Benzodiazepines
Osmolar Concentration
Surface properties
Contamination

Keywords

  • Drugs
  • Heart-cutting
  • Serum
  • Temperature-responsive chromatography
  • Two-dimensional liquid chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

The use of a temperature-responsive column for the direct analysis of drugs in serum by two-dimensional heart-cutting liquid chromatography. / Mikuma, Toshiyasu; Uchida, Ryo; Kajiya, Mizuki; Hiruta, Yuki; Kanazawa, Hideko.

In: Analytical and Bioanalytical Chemistry, Vol. 409, No. 4, 01.02.2017, p. 1059-1065.

Research output: Contribution to journalArticle

@article{2de9f401e26e464787e8a59b903e5f78,
title = "The use of a temperature-responsive column for the direct analysis of drugs in serum by two-dimensional heart-cutting liquid chromatography",
abstract = "A novel pretreatment method, which was performed using a two-dimensional high-performance liquid chromatography (2D-HPLC) system, was proposed for the direct analysis of drugs in human serum. A temperature-responsive column was used as a pretreatment column. The stationary phase of the temperature-responsive column exhibits temperature-regulated hydrophilic/hydrophobic characteristics. Controlling the ionic strength of the eluent enables human serum albumin (HSA) to pass through the column without retention. When serum samples containing barbiturates or benzodiazepines were injected into the temperature-responsive column using 10 mM of ammonium acetate (pH 6.5) as the mobile phase and in the temperature range of 10–40 °C, HSA was eluted from the column near the dead time, followed by the individual drugs. When the column temperature was changed, the retention times of the drugs were altered owing to surface property changes within the pretreatment column. These closely eluted compounds were subsequently introduced into the analytical column using a column-switching valve, with a minimal gap time to avoid foreign substance contamination. This new 2D-HPLC method afforded high-quality chromatograms of multiple drugs without unwanted peaks from foreign substances. The present technique could be an attractive choice in selecting the analytical method for drug analysis.",
keywords = "Drugs, Heart-cutting, Serum, Temperature-responsive chromatography, Two-dimensional liquid chromatography",
author = "Toshiyasu Mikuma and Ryo Uchida and Mizuki Kajiya and Yuki Hiruta and Hideko Kanazawa",
year = "2017",
month = "2",
day = "1",
doi = "10.1007/s00216-016-0024-9",
language = "English",
volume = "409",
pages = "1059--1065",
journal = "Fresenius Zeitschrift fur Analytische Chemie",
issn = "0016-1152",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - The use of a temperature-responsive column for the direct analysis of drugs in serum by two-dimensional heart-cutting liquid chromatography

AU - Mikuma, Toshiyasu

AU - Uchida, Ryo

AU - Kajiya, Mizuki

AU - Hiruta, Yuki

AU - Kanazawa, Hideko

PY - 2017/2/1

Y1 - 2017/2/1

N2 - A novel pretreatment method, which was performed using a two-dimensional high-performance liquid chromatography (2D-HPLC) system, was proposed for the direct analysis of drugs in human serum. A temperature-responsive column was used as a pretreatment column. The stationary phase of the temperature-responsive column exhibits temperature-regulated hydrophilic/hydrophobic characteristics. Controlling the ionic strength of the eluent enables human serum albumin (HSA) to pass through the column without retention. When serum samples containing barbiturates or benzodiazepines were injected into the temperature-responsive column using 10 mM of ammonium acetate (pH 6.5) as the mobile phase and in the temperature range of 10–40 °C, HSA was eluted from the column near the dead time, followed by the individual drugs. When the column temperature was changed, the retention times of the drugs were altered owing to surface property changes within the pretreatment column. These closely eluted compounds were subsequently introduced into the analytical column using a column-switching valve, with a minimal gap time to avoid foreign substance contamination. This new 2D-HPLC method afforded high-quality chromatograms of multiple drugs without unwanted peaks from foreign substances. The present technique could be an attractive choice in selecting the analytical method for drug analysis.

AB - A novel pretreatment method, which was performed using a two-dimensional high-performance liquid chromatography (2D-HPLC) system, was proposed for the direct analysis of drugs in human serum. A temperature-responsive column was used as a pretreatment column. The stationary phase of the temperature-responsive column exhibits temperature-regulated hydrophilic/hydrophobic characteristics. Controlling the ionic strength of the eluent enables human serum albumin (HSA) to pass through the column without retention. When serum samples containing barbiturates or benzodiazepines were injected into the temperature-responsive column using 10 mM of ammonium acetate (pH 6.5) as the mobile phase and in the temperature range of 10–40 °C, HSA was eluted from the column near the dead time, followed by the individual drugs. When the column temperature was changed, the retention times of the drugs were altered owing to surface property changes within the pretreatment column. These closely eluted compounds were subsequently introduced into the analytical column using a column-switching valve, with a minimal gap time to avoid foreign substance contamination. This new 2D-HPLC method afforded high-quality chromatograms of multiple drugs without unwanted peaks from foreign substances. The present technique could be an attractive choice in selecting the analytical method for drug analysis.

KW - Drugs

KW - Heart-cutting

KW - Serum

KW - Temperature-responsive chromatography

KW - Two-dimensional liquid chromatography

UR - http://www.scopus.com/inward/record.url?scp=84992694835&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84992694835&partnerID=8YFLogxK

U2 - 10.1007/s00216-016-0024-9

DO - 10.1007/s00216-016-0024-9

M3 - Article

C2 - 27796461

AN - SCOPUS:84992694835

VL - 409

SP - 1059

EP - 1065

JO - Fresenius Zeitschrift fur Analytische Chemie

JF - Fresenius Zeitschrift fur Analytische Chemie

SN - 0016-1152

IS - 4

ER -