The very-long-chain fatty acid elongase Elo2 rescues lethal defects associated with loss of the nuclear barrier function in fission yeast cells

Yasuha Kinugasa, Yasuhiro Hirano, Megumi Sawai, Yusuke Ohno, Tomoko Shindo, Haruhiko Asakawa, Yuji Chikashige, Shinsuke Shibata, Akio Kihara, Tokuko Haraguchi, Yasushi Hiraoka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In eukaryotic cells, chromosomes are confined to the nucleus, which is compartmentalized by the nuclear membranes; these are continuous with the endoplasmic reticulum membranes. Maintaining the homeostasis of these membranes is an important cellular activity performed by lipid metabolic enzymes. However, how lipid metabolic enzymes affect nuclear membrane functions remains to be elucidated. We found that the very-long-chain fatty acid elongase Elo2 is located in the nuclear membrane and prevents lethal defects associated with nuclear membrane ruptures in mutants of the nuclear membrane proteins Lem2 and Bqt4 in the fission yeast Schizosaccharomyces pombe. Lipid composition analysis shows that t20:0/24:0 phytoceramide (a conjugate of C20:0 phytosphingosine and C24:0 fatty acid) is a major ceramide species in S. pombe. The quantity of this ceramide is reduced in the absence of Lem2, and restored by increased expression of Elo2. Furthermore, loss of S. pombe Elo2 can be rescued by its human orthologs. These results suggest that the conserved very-long-chain fatty acid elongase producing the ceramide component is essential for nuclear membrane integrity and cell viability in eukaryotes.

Original languageEnglish
Article numberjcs229021
JournalJournal of Cell Science
Volume132
Issue number10
DOIs
Publication statusPublished - 2019 May 1

Fingerprint

Schizosaccharomyces
Nuclear Envelope
Ceramides
phytosphingosine
Lipids
Membranes
Eukaryotic Cells
Enzymes
Nuclear Proteins
Eukaryota
Endoplasmic Reticulum
fatty acid elongases
Rupture
Cell Survival
Membrane Proteins
Homeostasis
Fatty Acids
Chromosomes

Keywords

  • Elo2
  • Fatty acid elongation
  • Fission yeast
  • Lem2
  • Nuclear envelope

ASJC Scopus subject areas

  • Cell Biology

Cite this

The very-long-chain fatty acid elongase Elo2 rescues lethal defects associated with loss of the nuclear barrier function in fission yeast cells. / Kinugasa, Yasuha; Hirano, Yasuhiro; Sawai, Megumi; Ohno, Yusuke; Shindo, Tomoko; Asakawa, Haruhiko; Chikashige, Yuji; Shibata, Shinsuke; Kihara, Akio; Haraguchi, Tokuko; Hiraoka, Yasushi.

In: Journal of Cell Science, Vol. 132, No. 10, jcs229021, 01.05.2019.

Research output: Contribution to journalArticle

Kinugasa, Y, Hirano, Y, Sawai, M, Ohno, Y, Shindo, T, Asakawa, H, Chikashige, Y, Shibata, S, Kihara, A, Haraguchi, T & Hiraoka, Y 2019, 'The very-long-chain fatty acid elongase Elo2 rescues lethal defects associated with loss of the nuclear barrier function in fission yeast cells', Journal of Cell Science, vol. 132, no. 10, jcs229021. https://doi.org/10.1242/jcs.229021
Kinugasa, Yasuha ; Hirano, Yasuhiro ; Sawai, Megumi ; Ohno, Yusuke ; Shindo, Tomoko ; Asakawa, Haruhiko ; Chikashige, Yuji ; Shibata, Shinsuke ; Kihara, Akio ; Haraguchi, Tokuko ; Hiraoka, Yasushi. / The very-long-chain fatty acid elongase Elo2 rescues lethal defects associated with loss of the nuclear barrier function in fission yeast cells. In: Journal of Cell Science. 2019 ; Vol. 132, No. 10.
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