Thermally driven asymmetric domino reaction catalyzed by a thermostable esterase and its variants

Reina Wada, Takashi Kumon, Robert Kourist, Hiromichi Ohta, Daisuke Uemura, Shosuke Yoshida, Kenji Miyamoto

Research output: Contribution to journalArticle

7 Citations (Scopus)


We have developed a thermally driven domino reaction for the synthesis of (S)-a-arylpropionates (profens) using a thermostable esterase from Sulfolobus tokodaii strain 7. Stereoselectivity was improved considerably by engineering of the active site. Stereoselective decarboxylation at the active site of an esterase is a new reaction for the synthesis of optically active carboxylic acids. Crown Copyright.

Original languageEnglish
Pages (from-to)1921-1923
Number of pages3
JournalTetrahedron Letters
Issue number15
Publication statusPublished - 2013 Apr 10



  • A-Arylpropionates
  • Active site
  • Domino reaction
  • Thermal spontaneous decarboxylation
  • Thermostable esterase

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

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