Thermally modulated cationic copolymer brush on monolithic silica rods for high-speed separation of acidic biomolecules

Kenichi Nagase, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Hideko Kanazawa, Teruo Okano

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Poly(N-isopropylacrylamide (IPAAm)-co-2-(dimethylamino) ethylmethacrylate(DMAEMA)-co-tert-butylacrylamide (tBAAm)), a thermoresponsive-cationic-copolymer, brush-grafted monolithic-silica column was prepared through surface-initiated atom transfer radical polymerization (ATRP) for effective thermoresponsive anion-exchange chromatography matrices. ATRP-initiator was grafted on monolithic silica-rod surfaces by flowing a toluene solution containing ATRP initiator into monolithic silica-rod columns. IPAAm, DMAEMA, and tBAAm monomers and CuCl/CuCl2/Me6TREN, an ATRP catalytic system, were dissolved in 2-propanol, and the reaction solution was pumped into the preprepared initiator modified columns at 25 °C for 16 h. The constructed copolymer-brush structure on monolithic silica-rod surface was confirmed by X-ray photoelectron spectroscopy (XPS), elemental analysis, scanning electron microscopy (SEM) observation, and gel permeation chromatography (GPC) measurement of grafted copolymer. The prepared monolithic silica-rod columns were also characterized by chromatographic analysis. The cationic copolymer brush modified monolithic silica-rod columns were able to separate adenosine nucleotides with a shorter analysis time (4 min) than thermoresponsive copolymer brush-modified silica-bead-packed columns, because of the reduced diffusion path length of monolithic supporting materials. These results indicated that thermoresponsive cationic copolymer brush grafted monolithic silica-rod column prepared by ATRP was a promising tool for analyzing acidic-bioactive compounds with a remarkably short analysis time.

Original languageEnglish
Pages (from-to)1442-1452
Number of pages11
JournalACS Applied Materials and Interfaces
Volume5
Issue number4
DOIs
Publication statusPublished - 2013

Fingerprint

Biomolecules
Brushes
Silicon Dioxide
Copolymers
Silica
Atom transfer radical polymerization
Polymerization
Chromatography
Chromatographic analysis
Photoelectron Spectroscopy
2-Propanol
Toluene
Gel permeation chromatography
Propanol
Nucleotides
Electron Scanning Microscopy
Adenosine
Gel Chromatography
Anions
Ion exchange

Keywords

  • bioactive compounds
  • cationic polymer brush
  • chromatography
  • intelligent materials
  • poly(N -isopropylacrylamide)
  • temperature-responsive polymer

ASJC Scopus subject areas

  • Materials Science(all)
  • Medicine(all)

Cite this

Thermally modulated cationic copolymer brush on monolithic silica rods for high-speed separation of acidic biomolecules. / Nagase, Kenichi; Kobayashi, Jun; Kikuchi, Akihiko; Akiyama, Yoshikatsu; Kanazawa, Hideko; Okano, Teruo.

In: ACS Applied Materials and Interfaces, Vol. 5, No. 4, 2013, p. 1442-1452.

Research output: Contribution to journalArticle

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