Thermally Modulated Retention of Lymphoctytes on Polymer-Brush-Grafted Glass Beads

Kenichi Nagase; Naoto Mukae; Akihiko Kikuchi; Teruo Okano

doi:10.1002/mabi.201100283

Thermally Modulated Retention of Lymphoctytes on Polymer-Brush-Grafted Glass Beads

Kenichi Nagase, Naoto Mukae, Akihiko Kikuchi, Teruo Okano

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Thermoresponsive PIPAAm-brush-grafted glass beads are prepared through siATRP and their physicochemical properties are characterized by micro-nitrogen analysis, XPS, and contact angle measurements. The amount of grafted PIPAAm on glass bead surfaces can be controlled by varying the ATRP reaction time, leading to a modulation of the temperature-dependent wettability of the prepared surfaces. To evaluate a possible use of the beads as cell separation matrices, loading with rat lymphocytes from mesenteric lymph nodes is studied. The results show that the interaction between PIPAAm brushes and lymphocytes can be controlled by modulating PIPAAm brush length and temperature. The PIPAAm-brush-grafted beads might therefore be useful as effective cell separation matrices.

Original language	English
Pages (from-to)	333-340
Number of pages	8
Journal	Macromolecular Bioscience
Volume	12
Issue number	3
DOIs	https://doi.org/10.1002/mabi.201100283
Publication status	Published - 2012 Mar
Externally published	Yes

Keywords

Adhesion
Atom transfer radical polymerization (ATRP)
Cells
Polymer brushes
Stimuli-sensitive polymers

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

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10.1002/mabi.201100283

Cite this

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title = "Thermally Modulated Retention of Lymphoctytes on Polymer-Brush-Grafted Glass Beads",

abstract = "Thermoresponsive PIPAAm-brush-grafted glass beads are prepared through siATRP and their physicochemical properties are characterized by micro-nitrogen analysis, XPS, and contact angle measurements. The amount of grafted PIPAAm on glass bead surfaces can be controlled by varying the ATRP reaction time, leading to a modulation of the temperature-dependent wettability of the prepared surfaces. To evaluate a possible use of the beads as cell separation matrices, loading with rat lymphocytes from mesenteric lymph nodes is studied. The results show that the interaction between PIPAAm brushes and lymphocytes can be controlled by modulating PIPAAm brush length and temperature. The PIPAAm-brush-grafted beads might therefore be useful as effective cell separation matrices.",

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author = "Kenichi Nagase and Naoto Mukae and Akihiko Kikuchi and Teruo Okano",

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doi = "10.1002/mabi.201100283",

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AU - Nagase, Kenichi

AU - Mukae, Naoto

AU - Kikuchi, Akihiko

AU - Okano, Teruo

PY - 2012/3

Y1 - 2012/3

N2 - Thermoresponsive PIPAAm-brush-grafted glass beads are prepared through siATRP and their physicochemical properties are characterized by micro-nitrogen analysis, XPS, and contact angle measurements. The amount of grafted PIPAAm on glass bead surfaces can be controlled by varying the ATRP reaction time, leading to a modulation of the temperature-dependent wettability of the prepared surfaces. To evaluate a possible use of the beads as cell separation matrices, loading with rat lymphocytes from mesenteric lymph nodes is studied. The results show that the interaction between PIPAAm brushes and lymphocytes can be controlled by modulating PIPAAm brush length and temperature. The PIPAAm-brush-grafted beads might therefore be useful as effective cell separation matrices.

AB - Thermoresponsive PIPAAm-brush-grafted glass beads are prepared through siATRP and their physicochemical properties are characterized by micro-nitrogen analysis, XPS, and contact angle measurements. The amount of grafted PIPAAm on glass bead surfaces can be controlled by varying the ATRP reaction time, leading to a modulation of the temperature-dependent wettability of the prepared surfaces. To evaluate a possible use of the beads as cell separation matrices, loading with rat lymphocytes from mesenteric lymph nodes is studied. The results show that the interaction between PIPAAm brushes and lymphocytes can be controlled by modulating PIPAAm brush length and temperature. The PIPAAm-brush-grafted beads might therefore be useful as effective cell separation matrices.

KW - Adhesion

KW - Atom transfer radical polymerization (ATRP)

KW - Cells

KW - Polymer brushes

KW - Stimuli-sensitive polymers

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Thermally Modulated Retention of Lymphoctytes on Polymer-Brush-Grafted Glass Beads

Abstract

Keywords

ASJC Scopus subject areas

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