Thermo-Responsive Polymer Brushes as Intelligent Biointerfaces: Preparation via ATRP and Characterization

Kenichi Nagase, Minami Watanabe, Akihiko Kikuchi, Masayuki Yamato, Teruo Okano

Research output: Contribution to journalArticle

55 Citations (Scopus)


PIPAAm-brush grafted glass substrates with various graft densities and chain lengths were prepared via surface-initiated ATRP. Temperature-dependent physicochemical properties of the surfaces were characterized by means of ATR/FT-IR spectroscopy, XPS, AFM, and contact angle measurements. ATRP conditions influence the amount of grafted PIPAAm and the surface wettability and roughness of the substrate. Fibronectin adsorption and EC adhesion increased with decreasing density of PIPAAm brushes. EC adhesion was diminished with increasing PIPAAm graft length. Thus, the preparation of PIPAAm brush surface with various graft densities and chain lengths using the surface-initiated ATRP is an effective method for modulating thermo-responsive properties of surfaces. Thermo-responsive polymer brushes with various graft densities and chain lengths were prepared on glass substrates by surface-initiated ATRP. Cell adhesion and protein adsorption were enhanced with diluting polymer brush density, due to the exposure of grafted phenethyl group onto outer surfaces. These unique properties are valuable for designing novel biointerfaces.

Original languageEnglish
Pages (from-to)400-409
Number of pages10
JournalMacromolecular Bioscience
Issue number3
Publication statusPublished - 2011 Mar 10



  • Adhesion
  • Atom transfer radical polymerization (ATRP)
  • Cells
  • Polymer brushes
  • Stimuli-sensitive polymers

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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