Thermoresponsive copolymer brushes possessing quaternary amine groups for strong anion-exchange chromatographic matrices

Kenichi Nagase; Mike Geven; Saori Kimura; Jun Kobayashi; Akihiko Kikuchi; Yoshikatsu Akiyama; Dirk W. Grijpma; Hideko Kanazawa; Teruo Okano

doi:10.1021/bm401918a

Thermoresponsive copolymer brushes possessing quaternary amine groups for strong anion-exchange chromatographic matrices

Kenichi Nagase, Mike Geven, Saori Kimura, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Dirk W. Grijpma, Hideko Kanazawa, Teruo Okano

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

A thermoresponsive copolymer incorporating a quaternary amine group, poly(N-isopropylacrylamide-co-3-acrylamidopropyl trimethylammonium chloride (APTAC)-co-tert-butylacrylamide), was conjugated to the surface of silica beads through surface-initiated atom transfer radical polymerization. Prepared copolymer- and copolymer brush-modified beads were characterized by CHN elemental analysis, X-ray photoelectron spectroscopy, gel permeation chromatography, and observation of phase transition profiles. Phase transition profiles of the prepared copolymer indicated that 5 mol % APTAC is suitable for enabling thermally modulated property changes in the copolymer. Chromatographic elution behaviors of adenosine nucleotides and proteins were observed using prepared beads as chromatography matrices. Higher retention time of adenosine nucleotides and strong protein adsorption behavior were observed compared with those on beads with tertiary amine groups, because of the strong basic properties. Therefore, copolymer brush modified beads will be useful as thermoresponsive ion-exchange chromatographic matrices.

Original language	English
Pages (from-to)	1031-1043
Number of pages	13
Journal	Biomacromolecules
Volume	15
Issue number	3
DOIs	https://doi.org/10.1021/bm401918a
Publication status	Published - 2014 Mar 10
Externally published	Yes

ASJC Scopus subject areas

Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

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10.1021/bm401918a

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title = "Thermoresponsive copolymer brushes possessing quaternary amine groups for strong anion-exchange chromatographic matrices",

abstract = "A thermoresponsive copolymer incorporating a quaternary amine group, poly(N-isopropylacrylamide-co-3-acrylamidopropyl trimethylammonium chloride (APTAC)-co-tert-butylacrylamide), was conjugated to the surface of silica beads through surface-initiated atom transfer radical polymerization. Prepared copolymer- and copolymer brush-modified beads were characterized by CHN elemental analysis, X-ray photoelectron spectroscopy, gel permeation chromatography, and observation of phase transition profiles. Phase transition profiles of the prepared copolymer indicated that 5 mol % APTAC is suitable for enabling thermally modulated property changes in the copolymer. Chromatographic elution behaviors of adenosine nucleotides and proteins were observed using prepared beads as chromatography matrices. Higher retention time of adenosine nucleotides and strong protein adsorption behavior were observed compared with those on beads with tertiary amine groups, because of the strong basic properties. Therefore, copolymer brush modified beads will be useful as thermoresponsive ion-exchange chromatographic matrices.",

author = "Kenichi Nagase and Mike Geven and Saori Kimura and Jun Kobayashi and Akihiko Kikuchi and Yoshikatsu Akiyama and Grijpma, {Dirk W.} and Hideko Kanazawa and Teruo Okano",

year = "2014",

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doi = "10.1021/bm401918a",

language = "English",

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T1 - Thermoresponsive copolymer brushes possessing quaternary amine groups for strong anion-exchange chromatographic matrices

AU - Nagase, Kenichi

AU - Geven, Mike

AU - Kimura, Saori

AU - Kobayashi, Jun

AU - Kikuchi, Akihiko

AU - Akiyama, Yoshikatsu

AU - Grijpma, Dirk W.

AU - Kanazawa, Hideko

AU - Okano, Teruo

PY - 2014/3/10

Y1 - 2014/3/10

N2 - A thermoresponsive copolymer incorporating a quaternary amine group, poly(N-isopropylacrylamide-co-3-acrylamidopropyl trimethylammonium chloride (APTAC)-co-tert-butylacrylamide), was conjugated to the surface of silica beads through surface-initiated atom transfer radical polymerization. Prepared copolymer- and copolymer brush-modified beads were characterized by CHN elemental analysis, X-ray photoelectron spectroscopy, gel permeation chromatography, and observation of phase transition profiles. Phase transition profiles of the prepared copolymer indicated that 5 mol % APTAC is suitable for enabling thermally modulated property changes in the copolymer. Chromatographic elution behaviors of adenosine nucleotides and proteins were observed using prepared beads as chromatography matrices. Higher retention time of adenosine nucleotides and strong protein adsorption behavior were observed compared with those on beads with tertiary amine groups, because of the strong basic properties. Therefore, copolymer brush modified beads will be useful as thermoresponsive ion-exchange chromatographic matrices.

AB - A thermoresponsive copolymer incorporating a quaternary amine group, poly(N-isopropylacrylamide-co-3-acrylamidopropyl trimethylammonium chloride (APTAC)-co-tert-butylacrylamide), was conjugated to the surface of silica beads through surface-initiated atom transfer radical polymerization. Prepared copolymer- and copolymer brush-modified beads were characterized by CHN elemental analysis, X-ray photoelectron spectroscopy, gel permeation chromatography, and observation of phase transition profiles. Phase transition profiles of the prepared copolymer indicated that 5 mol % APTAC is suitable for enabling thermally modulated property changes in the copolymer. Chromatographic elution behaviors of adenosine nucleotides and proteins were observed using prepared beads as chromatography matrices. Higher retention time of adenosine nucleotides and strong protein adsorption behavior were observed compared with those on beads with tertiary amine groups, because of the strong basic properties. Therefore, copolymer brush modified beads will be useful as thermoresponsive ion-exchange chromatographic matrices.

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JO - Biomacromolecules

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Thermoresponsive copolymer brushes possessing quaternary amine groups for strong anion-exchange chromatographic matrices

Abstract

ASJC Scopus subject areas

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