Thermoresponsive copolymer brushes possessing quaternary amine groups for strong anion-exchange chromatographic matrices

Kenichi Nagase, Mike Geven, Saori Kimura, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Dirk W. Grijpma, Hideko Kanazawa, Teruo Okano

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A thermoresponsive copolymer incorporating a quaternary amine group, poly(N-isopropylacrylamide-co-3-acrylamidopropyl trimethylammonium chloride (APTAC)-co-tert-butylacrylamide), was conjugated to the surface of silica beads through surface-initiated atom transfer radical polymerization. Prepared copolymer- and copolymer brush-modified beads were characterized by CHN elemental analysis, X-ray photoelectron spectroscopy, gel permeation chromatography, and observation of phase transition profiles. Phase transition profiles of the prepared copolymer indicated that 5 mol % APTAC is suitable for enabling thermally modulated property changes in the copolymer. Chromatographic elution behaviors of adenosine nucleotides and proteins were observed using prepared beads as chromatography matrices. Higher retention time of adenosine nucleotides and strong protein adsorption behavior were observed compared with those on beads with tertiary amine groups, because of the strong basic properties. Therefore, copolymer brush modified beads will be useful as thermoresponsive ion-exchange chromatographic matrices.

Original languageEnglish
Pages (from-to)1031-1043
Number of pages13
JournalBiomacromolecules
Volume15
Issue number3
DOIs
Publication statusPublished - 2014 Mar 10

Fingerprint

Brushes
Amines
Anions
Ion exchange
Negative ions
Copolymers
Nucleotides
Adenosine
Phase transitions
Proteins
Atom transfer radical polymerization
Gel permeation chromatography
Chromatography
Silicon Dioxide
Chlorides
X ray photoelectron spectroscopy
Silica
Adsorption
Chemical analysis

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials

Cite this

Thermoresponsive copolymer brushes possessing quaternary amine groups for strong anion-exchange chromatographic matrices. / Nagase, Kenichi; Geven, Mike; Kimura, Saori; Kobayashi, Jun; Kikuchi, Akihiko; Akiyama, Yoshikatsu; Grijpma, Dirk W.; Kanazawa, Hideko; Okano, Teruo.

In: Biomacromolecules, Vol. 15, No. 3, 10.03.2014, p. 1031-1043.

Research output: Contribution to journalArticle

Nagase, Kenichi ; Geven, Mike ; Kimura, Saori ; Kobayashi, Jun ; Kikuchi, Akihiko ; Akiyama, Yoshikatsu ; Grijpma, Dirk W. ; Kanazawa, Hideko ; Okano, Teruo. / Thermoresponsive copolymer brushes possessing quaternary amine groups for strong anion-exchange chromatographic matrices. In: Biomacromolecules. 2014 ; Vol. 15, No. 3. pp. 1031-1043.
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AU - Akiyama, Yoshikatsu

AU - Grijpma, Dirk W.

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