Thermoresponsive copolymer brushes possessing quaternary amine groups for strong anion-exchange chromatographic matrices

Kenichi Nagase, Mike Geven, Saori Kimura, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Dirk W. Grijpma, Hideko Kanazawa, Teruo Okano

Research output: Contribution to journalArticle

21 Citations (Scopus)


A thermoresponsive copolymer incorporating a quaternary amine group, poly(N-isopropylacrylamide-co-3-acrylamidopropyl trimethylammonium chloride (APTAC)-co-tert-butylacrylamide), was conjugated to the surface of silica beads through surface-initiated atom transfer radical polymerization. Prepared copolymer- and copolymer brush-modified beads were characterized by CHN elemental analysis, X-ray photoelectron spectroscopy, gel permeation chromatography, and observation of phase transition profiles. Phase transition profiles of the prepared copolymer indicated that 5 mol % APTAC is suitable for enabling thermally modulated property changes in the copolymer. Chromatographic elution behaviors of adenosine nucleotides and proteins were observed using prepared beads as chromatography matrices. Higher retention time of adenosine nucleotides and strong protein adsorption behavior were observed compared with those on beads with tertiary amine groups, because of the strong basic properties. Therefore, copolymer brush modified beads will be useful as thermoresponsive ion-exchange chromatographic matrices.

Original languageEnglish
Pages (from-to)1031-1043
Number of pages13
Issue number3
Publication statusPublished - 2014 Mar 10


ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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