Thermoresponsive polymer-modified microfibers for cell separations

Kenichi Nagase, Yoichi Sakurada, Satoru Onizuka, Takanori Iwata, Masayuki Yamato, Naoya Takeda, Teruo Okano

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Thermoresponsive polymer-modified microfibers were prepared through electrospinning of poly(4-vinylbenzyl chloride) (PVBC) and subsequent surface-initiated atom transfer radical polymerization for grafting poly(N-isopropylacrylamide) (PIPAAm). Electrospinning conditions were optimized to produce large-diameter (20 μm) PVBC microfibers. The amount of PIPAAm grafted on the microfibers was controlled via the IPAAm monomer concentration. The microfibers exhibited thermally controlled cell separation by selective adhesion of normal human dermal fibroblasts in a mixed cell suspension that also contained human umbilical vein endothelial cells. In addition, adipose-derived stem cells (ADSCs) exhibited thermally modulated cell adhesion and detachment, while adhesion of other ADSC-related cells was low. Thus, ADSCs could be separated from a mixture of adipose tissue-derived cells simply by changing the temperature. Overall, the PIPAAm-modified microfibers are potentially applicable as temperature-modulated cell separation materials. Statement of Significance Thermoresponsive poly(N-isopropylacrylamide) (PIPAAm) polymer-modified poly(4-vinylbenzyl chloride) (PVBC) microfibers were prepared via electrospinning of PVBC, followed by surface-initiated ATRP. They formed effective thermally-modulated cell separation materials with large surface areas. Cells adhered and extended along the modified microfibers; this was not observed on previously reported PIPAAm-modified flat substrates. The cellular adhesion enabled separation of fibroblast cells, as well as that of adipose-derived mesenchymal stem cells, from mixtures of similar cells. Thus, the temperature-controlled thermoresponsive microfibers would be potentially useful as cell separation materials.

Original languageEnglish
Pages (from-to)81-92
Number of pages12
JournalActa Biomaterialia
Volume53
DOIs
Publication statusPublished - 2017 Apr 15
Externally publishedYes

Fingerprint

Cell Separation
Polymers
Stem cells
Chlorides
Electrospinning
Stem Cells
Adhesion
Atom transfer radical polymerization
Fibroblasts
Temperature
Cell adhesion
Endothelial cells
Human Umbilical Vein Endothelial Cells
Mesenchymal Stromal Cells
Cell Adhesion
Polymerization
Adipose Tissue
Suspensions
Monomers
Cells

Keywords

  • Cell separation
  • Electrospun microfiber
  • Polymer brush
  • Regenerative medicine
  • Stimuli responsive polymer

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering
  • Molecular Biology

Cite this

Nagase, K., Sakurada, Y., Onizuka, S., Iwata, T., Yamato, M., Takeda, N., & Okano, T. (2017). Thermoresponsive polymer-modified microfibers for cell separations. Acta Biomaterialia, 53, 81-92. https://doi.org/10.1016/j.actbio.2017.02.033

Thermoresponsive polymer-modified microfibers for cell separations. / Nagase, Kenichi; Sakurada, Yoichi; Onizuka, Satoru; Iwata, Takanori; Yamato, Masayuki; Takeda, Naoya; Okano, Teruo.

In: Acta Biomaterialia, Vol. 53, 15.04.2017, p. 81-92.

Research output: Contribution to journalArticle

Nagase, K, Sakurada, Y, Onizuka, S, Iwata, T, Yamato, M, Takeda, N & Okano, T 2017, 'Thermoresponsive polymer-modified microfibers for cell separations', Acta Biomaterialia, vol. 53, pp. 81-92. https://doi.org/10.1016/j.actbio.2017.02.033
Nagase, Kenichi ; Sakurada, Yoichi ; Onizuka, Satoru ; Iwata, Takanori ; Yamato, Masayuki ; Takeda, Naoya ; Okano, Teruo. / Thermoresponsive polymer-modified microfibers for cell separations. In: Acta Biomaterialia. 2017 ; Vol. 53. pp. 81-92.
@article{aa8adc7c6d17427d924cb907139b0b7b,
title = "Thermoresponsive polymer-modified microfibers for cell separations",
abstract = "Thermoresponsive polymer-modified microfibers were prepared through electrospinning of poly(4-vinylbenzyl chloride) (PVBC) and subsequent surface-initiated atom transfer radical polymerization for grafting poly(N-isopropylacrylamide) (PIPAAm). Electrospinning conditions were optimized to produce large-diameter (20 μm) PVBC microfibers. The amount of PIPAAm grafted on the microfibers was controlled via the IPAAm monomer concentration. The microfibers exhibited thermally controlled cell separation by selective adhesion of normal human dermal fibroblasts in a mixed cell suspension that also contained human umbilical vein endothelial cells. In addition, adipose-derived stem cells (ADSCs) exhibited thermally modulated cell adhesion and detachment, while adhesion of other ADSC-related cells was low. Thus, ADSCs could be separated from a mixture of adipose tissue-derived cells simply by changing the temperature. Overall, the PIPAAm-modified microfibers are potentially applicable as temperature-modulated cell separation materials. Statement of Significance Thermoresponsive poly(N-isopropylacrylamide) (PIPAAm) polymer-modified poly(4-vinylbenzyl chloride) (PVBC) microfibers were prepared via electrospinning of PVBC, followed by surface-initiated ATRP. They formed effective thermally-modulated cell separation materials with large surface areas. Cells adhered and extended along the modified microfibers; this was not observed on previously reported PIPAAm-modified flat substrates. The cellular adhesion enabled separation of fibroblast cells, as well as that of adipose-derived mesenchymal stem cells, from mixtures of similar cells. Thus, the temperature-controlled thermoresponsive microfibers would be potentially useful as cell separation materials.",
keywords = "Cell separation, Electrospun microfiber, Polymer brush, Regenerative medicine, Stimuli responsive polymer",
author = "Kenichi Nagase and Yoichi Sakurada and Satoru Onizuka and Takanori Iwata and Masayuki Yamato and Naoya Takeda and Teruo Okano",
year = "2017",
month = "4",
day = "15",
doi = "10.1016/j.actbio.2017.02.033",
language = "English",
volume = "53",
pages = "81--92",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Thermoresponsive polymer-modified microfibers for cell separations

AU - Nagase, Kenichi

AU - Sakurada, Yoichi

AU - Onizuka, Satoru

AU - Iwata, Takanori

AU - Yamato, Masayuki

AU - Takeda, Naoya

AU - Okano, Teruo

PY - 2017/4/15

Y1 - 2017/4/15

N2 - Thermoresponsive polymer-modified microfibers were prepared through electrospinning of poly(4-vinylbenzyl chloride) (PVBC) and subsequent surface-initiated atom transfer radical polymerization for grafting poly(N-isopropylacrylamide) (PIPAAm). Electrospinning conditions were optimized to produce large-diameter (20 μm) PVBC microfibers. The amount of PIPAAm grafted on the microfibers was controlled via the IPAAm monomer concentration. The microfibers exhibited thermally controlled cell separation by selective adhesion of normal human dermal fibroblasts in a mixed cell suspension that also contained human umbilical vein endothelial cells. In addition, adipose-derived stem cells (ADSCs) exhibited thermally modulated cell adhesion and detachment, while adhesion of other ADSC-related cells was low. Thus, ADSCs could be separated from a mixture of adipose tissue-derived cells simply by changing the temperature. Overall, the PIPAAm-modified microfibers are potentially applicable as temperature-modulated cell separation materials. Statement of Significance Thermoresponsive poly(N-isopropylacrylamide) (PIPAAm) polymer-modified poly(4-vinylbenzyl chloride) (PVBC) microfibers were prepared via electrospinning of PVBC, followed by surface-initiated ATRP. They formed effective thermally-modulated cell separation materials with large surface areas. Cells adhered and extended along the modified microfibers; this was not observed on previously reported PIPAAm-modified flat substrates. The cellular adhesion enabled separation of fibroblast cells, as well as that of adipose-derived mesenchymal stem cells, from mixtures of similar cells. Thus, the temperature-controlled thermoresponsive microfibers would be potentially useful as cell separation materials.

AB - Thermoresponsive polymer-modified microfibers were prepared through electrospinning of poly(4-vinylbenzyl chloride) (PVBC) and subsequent surface-initiated atom transfer radical polymerization for grafting poly(N-isopropylacrylamide) (PIPAAm). Electrospinning conditions were optimized to produce large-diameter (20 μm) PVBC microfibers. The amount of PIPAAm grafted on the microfibers was controlled via the IPAAm monomer concentration. The microfibers exhibited thermally controlled cell separation by selective adhesion of normal human dermal fibroblasts in a mixed cell suspension that also contained human umbilical vein endothelial cells. In addition, adipose-derived stem cells (ADSCs) exhibited thermally modulated cell adhesion and detachment, while adhesion of other ADSC-related cells was low. Thus, ADSCs could be separated from a mixture of adipose tissue-derived cells simply by changing the temperature. Overall, the PIPAAm-modified microfibers are potentially applicable as temperature-modulated cell separation materials. Statement of Significance Thermoresponsive poly(N-isopropylacrylamide) (PIPAAm) polymer-modified poly(4-vinylbenzyl chloride) (PVBC) microfibers were prepared via electrospinning of PVBC, followed by surface-initiated ATRP. They formed effective thermally-modulated cell separation materials with large surface areas. Cells adhered and extended along the modified microfibers; this was not observed on previously reported PIPAAm-modified flat substrates. The cellular adhesion enabled separation of fibroblast cells, as well as that of adipose-derived mesenchymal stem cells, from mixtures of similar cells. Thus, the temperature-controlled thermoresponsive microfibers would be potentially useful as cell separation materials.

KW - Cell separation

KW - Electrospun microfiber

KW - Polymer brush

KW - Regenerative medicine

KW - Stimuli responsive polymer

UR - http://www.scopus.com/inward/record.url?scp=85014074252&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85014074252&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2017.02.033

DO - 10.1016/j.actbio.2017.02.033

M3 - Article

C2 - 28219809

AN - SCOPUS:85014074252

VL - 53

SP - 81

EP - 92

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

ER -