Thermoresponsive Poly(N-isopropylacrylamide)-Based Block Copolymer Coating for Optimizing Cell Sheet Fabrication

Masamichi Nakayama; Naoko Yamada; Yoshikazu Kumashiro; Hideko Kanazawa; Masayuki Yamato; Teruo Okano

doi:10.1002/mabi.201200018

Thermoresponsive Poly(N-isopropylacrylamide)-Based Block Copolymer Coating for Optimizing Cell Sheet Fabrication

Masamichi Nakayama, Naoko Yamada, Yoshikazu Kumashiro, Hideko Kanazawa, Masayuki Yamato, Teruo Okano

School of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

Thermoresponsive surfaces are prepared via a spin-coating method with a block copolymer consisting of poly(N-isopropylacrylamide) (PIPAAm) and poly(butyl methacrylate) (PBMA) on polystyrene surfaces. The PBMA block suppresses the removal of deposited PIPAAm-based polymers from the surface. The polymer coating affects the temperature-dependent cellular behavior of the surfaces with respect to protein adsorption. By adjusting layer thicknesses, PBMA-b-PIPAAm-coated surfaces are optimized to regulate the adhesion/detachment of cells by temperature changes. Thus, thermoresponsive polymer-coated surfaces are able to harvest contiguous cell sheets with their basal extracellular matrix proteins.

Original language	English
Pages (from-to)	751-760
Number of pages	10
Journal	Macromolecular Bioscience
Volume	12
Issue number	6
DOIs	https://doi.org/10.1002/mabi.201200018
Publication status	Published - 2012 Jun

Keywords

Adhesion
Block copolymers
Cells
Poly(N-isopropylacrylamide)
Spin coating

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

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10.1002/mabi.201200018

Cite this

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abstract = "Thermoresponsive surfaces are prepared via a spin-coating method with a block copolymer consisting of poly(N-isopropylacrylamide) (PIPAAm) and poly(butyl methacrylate) (PBMA) on polystyrene surfaces. The PBMA block suppresses the removal of deposited PIPAAm-based polymers from the surface. The polymer coating affects the temperature-dependent cellular behavior of the surfaces with respect to protein adsorption. By adjusting layer thicknesses, PBMA-b-PIPAAm-coated surfaces are optimized to regulate the adhesion/detachment of cells by temperature changes. Thus, thermoresponsive polymer-coated surfaces are able to harvest contiguous cell sheets with their basal extracellular matrix proteins.",

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AU - Nakayama, Masamichi

AU - Yamada, Naoko

AU - Kumashiro, Yoshikazu

AU - Kanazawa, Hideko

AU - Yamato, Masayuki

AU - Okano, Teruo

PY - 2012/6

Y1 - 2012/6

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AB - Thermoresponsive surfaces are prepared via a spin-coating method with a block copolymer consisting of poly(N-isopropylacrylamide) (PIPAAm) and poly(butyl methacrylate) (PBMA) on polystyrene surfaces. The PBMA block suppresses the removal of deposited PIPAAm-based polymers from the surface. The polymer coating affects the temperature-dependent cellular behavior of the surfaces with respect to protein adsorption. By adjusting layer thicknesses, PBMA-b-PIPAAm-coated surfaces are optimized to regulate the adhesion/detachment of cells by temperature changes. Thus, thermoresponsive polymer-coated surfaces are able to harvest contiguous cell sheets with their basal extracellular matrix proteins.

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KW - Cells

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KW - Spin coating

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Thermoresponsive Poly(N-isopropylacrylamide)-Based Block Copolymer Coating for Optimizing Cell Sheet Fabrication

Abstract

Keywords

ASJC Scopus subject areas

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