Abstract
Oxidative stress has a bidirectional role in the development and maturation of zygotes and embryos. Reduction-oxidation reactions and regulatory proteins, such as thioredoxin (TRX) and thioredoxin reductase (TRXR), are intimately involved in the regulation of oxidative stress. The aim of this study was to determine the levels of TRX mRNA and protein in ovarian follicles collected from women undergoing in vitro fertilization (IVF) and to assess these levels relative to follicle size, presence of oocytes, and responsiveness to superovulation. Follicular fluid (FF) and/or granulosa cells (GCs) from large and small follicles were collected at the time of ovum pick-up from 42 IVF patients enrolled in this study. We divided the patients into normal and poor responders (NR and PR, respectively) based on the serum estradiol levels on the day of human chorionic gonadotropin (hCG) administration. We also compared the TRX concentration in FF (FF-TRX) between oocyte-containing follicles (Oc+) and empty follicles (Oc-). The transcript levels of TRX, but not TRXR, were significantly higher in GCs derived from follicles collected from NR than PR, as determined by semi-quantitative RT-PCR analysis. In NR, the FF-TRX was significantly higher in Oc+ follicles than in Ocfollicles and also in large Oc+ follicles than in large Oc-follicles. Unlike NR, PR exhibited no positive association with elevated FF-TRX and presence of oocytes. Based on its collective anti-oxidative, cytoprotective, and cytokine-like properties of TRX, TRX is likely to be involved in the optimal growth and maturation of ovarian follicles and responsiveness to hyperstimulation.
| Original language | English |
|---|---|
| Pages (from-to) | 9-20 |
| Number of pages | 12 |
| Journal | Endocrine Journal |
| Volume | 63 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2016 Jan 31 |
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Keywords
- Follicular fluids
- IVF
- Ovarian follicles
- Poor responders
- Thioredoxin
ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
Cite this
Thioredoxin, an antioxidant redox protein, in ovarian follicles of women undergoing in vitro fertilization. / Kishi, Ikuko; Ohishi, Miho; Akiba, Yasuo; Asada, Hironori; Konishi, Yasuhiro; Nakano, Masao; Kamei, Kiyoshi; Yoshimura, Yasunori; Maruyama, Tetsuo.
In: Endocrine Journal, Vol. 63, No. 1, 31.01.2016, p. 9-20.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Thioredoxin, an antioxidant redox protein, in ovarian follicles of women undergoing in vitro fertilization
AU - Kishi, Ikuko
AU - Ohishi, Miho
AU - Akiba, Yasuo
AU - Asada, Hironori
AU - Konishi, Yasuhiro
AU - Nakano, Masao
AU - Kamei, Kiyoshi
AU - Yoshimura, Yasunori
AU - Maruyama, Tetsuo
PY - 2016/1/31
Y1 - 2016/1/31
N2 - Oxidative stress has a bidirectional role in the development and maturation of zygotes and embryos. Reduction-oxidation reactions and regulatory proteins, such as thioredoxin (TRX) and thioredoxin reductase (TRXR), are intimately involved in the regulation of oxidative stress. The aim of this study was to determine the levels of TRX mRNA and protein in ovarian follicles collected from women undergoing in vitro fertilization (IVF) and to assess these levels relative to follicle size, presence of oocytes, and responsiveness to superovulation. Follicular fluid (FF) and/or granulosa cells (GCs) from large and small follicles were collected at the time of ovum pick-up from 42 IVF patients enrolled in this study. We divided the patients into normal and poor responders (NR and PR, respectively) based on the serum estradiol levels on the day of human chorionic gonadotropin (hCG) administration. We also compared the TRX concentration in FF (FF-TRX) between oocyte-containing follicles (Oc+) and empty follicles (Oc-). The transcript levels of TRX, but not TRXR, were significantly higher in GCs derived from follicles collected from NR than PR, as determined by semi-quantitative RT-PCR analysis. In NR, the FF-TRX was significantly higher in Oc+ follicles than in Ocfollicles and also in large Oc+ follicles than in large Oc-follicles. Unlike NR, PR exhibited no positive association with elevated FF-TRX and presence of oocytes. Based on its collective anti-oxidative, cytoprotective, and cytokine-like properties of TRX, TRX is likely to be involved in the optimal growth and maturation of ovarian follicles and responsiveness to hyperstimulation.
AB - Oxidative stress has a bidirectional role in the development and maturation of zygotes and embryos. Reduction-oxidation reactions and regulatory proteins, such as thioredoxin (TRX) and thioredoxin reductase (TRXR), are intimately involved in the regulation of oxidative stress. The aim of this study was to determine the levels of TRX mRNA and protein in ovarian follicles collected from women undergoing in vitro fertilization (IVF) and to assess these levels relative to follicle size, presence of oocytes, and responsiveness to superovulation. Follicular fluid (FF) and/or granulosa cells (GCs) from large and small follicles were collected at the time of ovum pick-up from 42 IVF patients enrolled in this study. We divided the patients into normal and poor responders (NR and PR, respectively) based on the serum estradiol levels on the day of human chorionic gonadotropin (hCG) administration. We also compared the TRX concentration in FF (FF-TRX) between oocyte-containing follicles (Oc+) and empty follicles (Oc-). The transcript levels of TRX, but not TRXR, were significantly higher in GCs derived from follicles collected from NR than PR, as determined by semi-quantitative RT-PCR analysis. In NR, the FF-TRX was significantly higher in Oc+ follicles than in Ocfollicles and also in large Oc+ follicles than in large Oc-follicles. Unlike NR, PR exhibited no positive association with elevated FF-TRX and presence of oocytes. Based on its collective anti-oxidative, cytoprotective, and cytokine-like properties of TRX, TRX is likely to be involved in the optimal growth and maturation of ovarian follicles and responsiveness to hyperstimulation.
KW - Follicular fluids
KW - IVF
KW - Ovarian follicles
KW - Poor responders
KW - Thioredoxin
UR - http://www.scopus.com/inward/record.url?scp=84956792861&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84956792861&partnerID=8YFLogxK
U2 - 10.1507/endocrj.EJ15-0210
DO - 10.1507/endocrj.EJ15-0210
M3 - Article
C2 - 26536897
AN - SCOPUS:84956792861
VL - 63
SP - 9
EP - 20
JO - Endocrine Journal
JF - Endocrine Journal
SN - 0918-8959
IS - 1
ER -