Thioredoxin expression in the human endometrium during the menstrual cycle

Tetsuo Maruyama, Yuki Kitaoka, Yoshifumi Sachi, Kunihiko Nakanoin, Kiichi Hirota, Tanri Shiozawa, Yasunori Yoshimura, Shingo Fujii, Junji Yodoi

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Human thioredoxin, a cellular disulphide reducing protein, is known to be secreted by some types of cells and to display unique extracellular activities including modulation of cytokine actions and protection of the cell against damage from oxidative stress. This study has been undertaken to investigate the pattern of expression and tissue distribution of thioredoxin in human endometrium during the menstrual cycle. Immunohistochemical studies showed increased thioredoxin immunoreactivity in the glands of the secretory phase compared to those of the proliferative phase. Although the staining of thioredoxin was relatively intense in predecidual stromal cells, the most prominent staining of thioredoxin was present in both glands and stroma of the endometrium in the early secretory phase of the menstrual cycle. Northern hybridization analyses revealed that expression of thioredoxin mRNA in the endometrium of the early secretory phase was ∼3-fold compared to the other phases of the menstrual cycle, consistent with the results of the immunohistochemical studies. These results suggest that both protein and gene expression of thioredoxin in the endometrium are menstrual cycle phase-specific and highly active in the phase of endometrial differentiation which occurs in preparation for implantation (early secretory phase of the menstrual cycle). Thioredoxin expressed in the early secretory phase of the menstrual cycle may be advantageous for blastocyst implantation.

Original languageEnglish
Pages (from-to)989-993
Number of pages5
JournalMolecular Human Reproduction
Volume3
Issue number11
Publication statusPublished - 1997

Fingerprint

Thioredoxins
Menstrual Cycle
Endometrium
Luteal Phase
Staining and Labeling
Cytoprotection
Tissue Distribution
Stromal Cells
Disulfides
Proteins
Oxidative Stress
Cytokines
Gene Expression
Messenger RNA

Keywords

  • Endometrium
  • Menstrual cycle
  • Predecidual cells
  • Thioredoxin

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Genetics
  • Embryology
  • Obstetrics and Gynaecology

Cite this

Maruyama, T., Kitaoka, Y., Sachi, Y., Nakanoin, K., Hirota, K., Shiozawa, T., ... Yodoi, J. (1997). Thioredoxin expression in the human endometrium during the menstrual cycle. Molecular Human Reproduction, 3(11), 989-993.

Thioredoxin expression in the human endometrium during the menstrual cycle. / Maruyama, Tetsuo; Kitaoka, Yuki; Sachi, Yoshifumi; Nakanoin, Kunihiko; Hirota, Kiichi; Shiozawa, Tanri; Yoshimura, Yasunori; Fujii, Shingo; Yodoi, Junji.

In: Molecular Human Reproduction, Vol. 3, No. 11, 1997, p. 989-993.

Research output: Contribution to journalArticle

Maruyama, T, Kitaoka, Y, Sachi, Y, Nakanoin, K, Hirota, K, Shiozawa, T, Yoshimura, Y, Fujii, S & Yodoi, J 1997, 'Thioredoxin expression in the human endometrium during the menstrual cycle', Molecular Human Reproduction, vol. 3, no. 11, pp. 989-993.
Maruyama T, Kitaoka Y, Sachi Y, Nakanoin K, Hirota K, Shiozawa T et al. Thioredoxin expression in the human endometrium during the menstrual cycle. Molecular Human Reproduction. 1997;3(11):989-993.
Maruyama, Tetsuo ; Kitaoka, Yuki ; Sachi, Yoshifumi ; Nakanoin, Kunihiko ; Hirota, Kiichi ; Shiozawa, Tanri ; Yoshimura, Yasunori ; Fujii, Shingo ; Yodoi, Junji. / Thioredoxin expression in the human endometrium during the menstrual cycle. In: Molecular Human Reproduction. 1997 ; Vol. 3, No. 11. pp. 989-993.
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