Thioredoxin may exert a protective effect against tissue damage caused by oxidative stress in salivary glands of patients with Sjögren's syndrome

Chiyo Kurimoto, Seiji Kawano, Goh Tsuji, Saori Hatachi, Takumi Jikimoto, Daisuke Sugiyama, Shimpei Kasagi, Takahide Komori, Hajime Nakamura, Junji Yodoi, Shunichi Kumagai

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Objective. To demonstrate the existence of oxidative stress and the role of the antioxidant thioredoxin (TRX) in Sjögren's syndrome (SS). Methods. Labial biopsy specimens from patients with SS were analyzed immunohistochemically to detect 8-hydroxy-2′-deoxyguanosine (8-OHdG), 4-hydroxy-2-nonenal (4-HNE), nitrotyrosine, and TRX. Levels of TRX in saliva and plasma were quantified by ELISA. To analyze the effect of TRX on human salivary gland (HSG) cells, recombinant TRX (rTRX)-treated HSG cells were stimulated by interferon-γ (IFN-γ) for detecting interleukin 6 (IL-6) with ELISA and RT-PCR, or stimulated with IFN-γ and anti-Fas antibody for analyzing Fas-induced apoptosis with PI/annexin V staining. Results. Large amounts of 8-OHdG, 4-HNE, nitrotyrosine, and TRX were produced in salivary duct cells of SS patients, whether there was periductal lymphocytic infiltration or not. Strong TRX expression was detected in acinar cells from 13 of 19 SS specimens. Levels of salivary TRX were significantly higher in SS patients than in controls (p < 0.05), and were inversely related to the salivary flow rates in SS patients. Patients who showed acinar TRX expression had higher salivary TRX levels than those who did not (p < 0.05). Interferon-γ-induced expression of IL-6 and Fas-mediated apoptosis in HSG cells were significantly suppressed by pretreating cells with rTRX. Conclusion. Parallel production of oxidative stress markers together with massive secretion of TRX suggests that oxidative stress induces TRX in the salivary gland. Moreover, suppression of IL-6 production and apoptosis by rTRX in HSG cells suggests TRX acts to protect the salivary glands of SS patients from tissue damage.

Original languageEnglish
Pages (from-to)2035-2043
Number of pages9
JournalJournal of Rheumatology
Volume34
Issue number10
Publication statusPublished - 2007 Oct
Externally publishedYes

Fingerprint

Thioredoxins
Salivary Glands
Oxidative Stress
Interferons
Interleukin-6
Apoptosis
Enzyme-Linked Immunosorbent Assay
Salivary Ducts
Acinar Cells
Annexin A5
Lip
Saliva
Anti-Idiotypic Antibodies

Keywords

  • 8-OHdG
  • Ductal cell
  • Oxidative stress
  • Sjögren's syndrome
  • Thioredoxin

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Thioredoxin may exert a protective effect against tissue damage caused by oxidative stress in salivary glands of patients with Sjögren's syndrome. / Kurimoto, Chiyo; Kawano, Seiji; Tsuji, Goh; Hatachi, Saori; Jikimoto, Takumi; Sugiyama, Daisuke; Kasagi, Shimpei; Komori, Takahide; Nakamura, Hajime; Yodoi, Junji; Kumagai, Shunichi.

In: Journal of Rheumatology, Vol. 34, No. 10, 10.2007, p. 2035-2043.

Research output: Contribution to journalArticle

Kurimoto, C, Kawano, S, Tsuji, G, Hatachi, S, Jikimoto, T, Sugiyama, D, Kasagi, S, Komori, T, Nakamura, H, Yodoi, J & Kumagai, S 2007, 'Thioredoxin may exert a protective effect against tissue damage caused by oxidative stress in salivary glands of patients with Sjögren's syndrome', Journal of Rheumatology, vol. 34, no. 10, pp. 2035-2043.
Kurimoto, Chiyo ; Kawano, Seiji ; Tsuji, Goh ; Hatachi, Saori ; Jikimoto, Takumi ; Sugiyama, Daisuke ; Kasagi, Shimpei ; Komori, Takahide ; Nakamura, Hajime ; Yodoi, Junji ; Kumagai, Shunichi. / Thioredoxin may exert a protective effect against tissue damage caused by oxidative stress in salivary glands of patients with Sjögren's syndrome. In: Journal of Rheumatology. 2007 ; Vol. 34, No. 10. pp. 2035-2043.
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abstract = "Objective. To demonstrate the existence of oxidative stress and the role of the antioxidant thioredoxin (TRX) in Sj{\"o}gren's syndrome (SS). Methods. Labial biopsy specimens from patients with SS were analyzed immunohistochemically to detect 8-hydroxy-2′-deoxyguanosine (8-OHdG), 4-hydroxy-2-nonenal (4-HNE), nitrotyrosine, and TRX. Levels of TRX in saliva and plasma were quantified by ELISA. To analyze the effect of TRX on human salivary gland (HSG) cells, recombinant TRX (rTRX)-treated HSG cells were stimulated by interferon-γ (IFN-γ) for detecting interleukin 6 (IL-6) with ELISA and RT-PCR, or stimulated with IFN-γ and anti-Fas antibody for analyzing Fas-induced apoptosis with PI/annexin V staining. Results. Large amounts of 8-OHdG, 4-HNE, nitrotyrosine, and TRX were produced in salivary duct cells of SS patients, whether there was periductal lymphocytic infiltration or not. Strong TRX expression was detected in acinar cells from 13 of 19 SS specimens. Levels of salivary TRX were significantly higher in SS patients than in controls (p < 0.05), and were inversely related to the salivary flow rates in SS patients. Patients who showed acinar TRX expression had higher salivary TRX levels than those who did not (p < 0.05). Interferon-γ-induced expression of IL-6 and Fas-mediated apoptosis in HSG cells were significantly suppressed by pretreating cells with rTRX. Conclusion. Parallel production of oxidative stress markers together with massive secretion of TRX suggests that oxidative stress induces TRX in the salivary gland. Moreover, suppression of IL-6 production and apoptosis by rTRX in HSG cells suggests TRX acts to protect the salivary glands of SS patients from tissue damage.",
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AU - Kurimoto, Chiyo

AU - Kawano, Seiji

AU - Tsuji, Goh

AU - Hatachi, Saori

AU - Jikimoto, Takumi

AU - Sugiyama, Daisuke

AU - Kasagi, Shimpei

AU - Komori, Takahide

AU - Nakamura, Hajime

AU - Yodoi, Junji

AU - Kumagai, Shunichi

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N2 - Objective. To demonstrate the existence of oxidative stress and the role of the antioxidant thioredoxin (TRX) in Sjögren's syndrome (SS). Methods. Labial biopsy specimens from patients with SS were analyzed immunohistochemically to detect 8-hydroxy-2′-deoxyguanosine (8-OHdG), 4-hydroxy-2-nonenal (4-HNE), nitrotyrosine, and TRX. Levels of TRX in saliva and plasma were quantified by ELISA. To analyze the effect of TRX on human salivary gland (HSG) cells, recombinant TRX (rTRX)-treated HSG cells were stimulated by interferon-γ (IFN-γ) for detecting interleukin 6 (IL-6) with ELISA and RT-PCR, or stimulated with IFN-γ and anti-Fas antibody for analyzing Fas-induced apoptosis with PI/annexin V staining. Results. Large amounts of 8-OHdG, 4-HNE, nitrotyrosine, and TRX were produced in salivary duct cells of SS patients, whether there was periductal lymphocytic infiltration or not. Strong TRX expression was detected in acinar cells from 13 of 19 SS specimens. Levels of salivary TRX were significantly higher in SS patients than in controls (p < 0.05), and were inversely related to the salivary flow rates in SS patients. Patients who showed acinar TRX expression had higher salivary TRX levels than those who did not (p < 0.05). Interferon-γ-induced expression of IL-6 and Fas-mediated apoptosis in HSG cells were significantly suppressed by pretreating cells with rTRX. Conclusion. Parallel production of oxidative stress markers together with massive secretion of TRX suggests that oxidative stress induces TRX in the salivary gland. Moreover, suppression of IL-6 production and apoptosis by rTRX in HSG cells suggests TRX acts to protect the salivary glands of SS patients from tissue damage.

AB - Objective. To demonstrate the existence of oxidative stress and the role of the antioxidant thioredoxin (TRX) in Sjögren's syndrome (SS). Methods. Labial biopsy specimens from patients with SS were analyzed immunohistochemically to detect 8-hydroxy-2′-deoxyguanosine (8-OHdG), 4-hydroxy-2-nonenal (4-HNE), nitrotyrosine, and TRX. Levels of TRX in saliva and plasma were quantified by ELISA. To analyze the effect of TRX on human salivary gland (HSG) cells, recombinant TRX (rTRX)-treated HSG cells were stimulated by interferon-γ (IFN-γ) for detecting interleukin 6 (IL-6) with ELISA and RT-PCR, or stimulated with IFN-γ and anti-Fas antibody for analyzing Fas-induced apoptosis with PI/annexin V staining. Results. Large amounts of 8-OHdG, 4-HNE, nitrotyrosine, and TRX were produced in salivary duct cells of SS patients, whether there was periductal lymphocytic infiltration or not. Strong TRX expression was detected in acinar cells from 13 of 19 SS specimens. Levels of salivary TRX were significantly higher in SS patients than in controls (p < 0.05), and were inversely related to the salivary flow rates in SS patients. Patients who showed acinar TRX expression had higher salivary TRX levels than those who did not (p < 0.05). Interferon-γ-induced expression of IL-6 and Fas-mediated apoptosis in HSG cells were significantly suppressed by pretreating cells with rTRX. Conclusion. Parallel production of oxidative stress markers together with massive secretion of TRX suggests that oxidative stress induces TRX in the salivary gland. Moreover, suppression of IL-6 production and apoptosis by rTRX in HSG cells suggests TRX acts to protect the salivary glands of SS patients from tissue damage.

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