Three-dimensional anatomy of the Ciona intestinalis tailbud embryo at single-cell resolution

Mitsuru J. Nakamura, Jun Terai, Reiko Okubo, Kohji Hotta, Kotaro Oka

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

During embryogenesis, chordates pass through a tailbud stage in which the larval tail is formed. Since acquisition of a tadpole-like tail during tailbud stage is one of the key events in the evolution of chordates, understanding the anatomy of the tailbud stage chordate embryo is of special interest. In this study, to understand comprehensively the anatomy of the tailbud embryo at single-cell-level, real microscopic image stacks of the tailbud embryo in Ciona intestinalis were reconstructed into a 3D computer model. This comprehensive 3D model of the ascidian tailbud embryo was based on real images of confocal laser scanning microscope (CLSM) and therefore, cell shape, location and cell arrangement reflect real geometries of the tailbud embryo. We found that the tailbud embryo consists of 1579 cells, including 836 epidermal cells, 228 cells in the central nervous system, 218 mesenchymal cells, four trunk ventral cells, two B/B*8.11 cells, 36 muscle cells, 40 notochord cells, four primordial germ cells, and 199 endodermal cells. Moreover, we identified for the first time two populations of previously undefined cells (a total of 12 cells) in Ciona: one located in the lateral trunk and the other located under the tail dorsal epidermis. This information provides a first step for understanding how the body plan of the chordate tailbud embryo formed and evolved.

Original languageEnglish
Pages (from-to)274-284
Number of pages11
JournalDevelopmental Biology
Volume372
Issue number2
DOIs
Publication statusPublished - 2012 Dec 15

Fingerprint

Ciona intestinalis
Anatomy
Embryonic Structures
Chordata
Tail
Notochord
Urochordata
Cell Shape
Epidermis
Germ Cells
Computer Simulation
Muscle Cells
Embryonic Development
Larva

Keywords

  • Anatomy
  • Cell lineage
  • Chordate evolution
  • Interactive 3D visualization
  • Segmentation
  • Tunicate

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Three-dimensional anatomy of the Ciona intestinalis tailbud embryo at single-cell resolution. / Nakamura, Mitsuru J.; Terai, Jun; Okubo, Reiko; Hotta, Kohji; Oka, Kotaro.

In: Developmental Biology, Vol. 372, No. 2, 15.12.2012, p. 274-284.

Research output: Contribution to journalArticle

Nakamura, Mitsuru J. ; Terai, Jun ; Okubo, Reiko ; Hotta, Kohji ; Oka, Kotaro. / Three-dimensional anatomy of the Ciona intestinalis tailbud embryo at single-cell resolution. In: Developmental Biology. 2012 ; Vol. 372, No. 2. pp. 274-284.
@article{dd3ce8001c384d7c8a37c3bfe058964a,
title = "Three-dimensional anatomy of the Ciona intestinalis tailbud embryo at single-cell resolution",
abstract = "During embryogenesis, chordates pass through a tailbud stage in which the larval tail is formed. Since acquisition of a tadpole-like tail during tailbud stage is one of the key events in the evolution of chordates, understanding the anatomy of the tailbud stage chordate embryo is of special interest. In this study, to understand comprehensively the anatomy of the tailbud embryo at single-cell-level, real microscopic image stacks of the tailbud embryo in Ciona intestinalis were reconstructed into a 3D computer model. This comprehensive 3D model of the ascidian tailbud embryo was based on real images of confocal laser scanning microscope (CLSM) and therefore, cell shape, location and cell arrangement reflect real geometries of the tailbud embryo. We found that the tailbud embryo consists of 1579 cells, including 836 epidermal cells, 228 cells in the central nervous system, 218 mesenchymal cells, four trunk ventral cells, two B/B*8.11 cells, 36 muscle cells, 40 notochord cells, four primordial germ cells, and 199 endodermal cells. Moreover, we identified for the first time two populations of previously undefined cells (a total of 12 cells) in Ciona: one located in the lateral trunk and the other located under the tail dorsal epidermis. This information provides a first step for understanding how the body plan of the chordate tailbud embryo formed and evolved.",
keywords = "Anatomy, Cell lineage, Chordate evolution, Interactive 3D visualization, Segmentation, Tunicate",
author = "Nakamura, {Mitsuru J.} and Jun Terai and Reiko Okubo and Kohji Hotta and Kotaro Oka",
year = "2012",
month = "12",
day = "15",
doi = "10.1016/j.ydbio.2012.09.007",
language = "English",
volume = "372",
pages = "274--284",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Three-dimensional anatomy of the Ciona intestinalis tailbud embryo at single-cell resolution

AU - Nakamura, Mitsuru J.

AU - Terai, Jun

AU - Okubo, Reiko

AU - Hotta, Kohji

AU - Oka, Kotaro

PY - 2012/12/15

Y1 - 2012/12/15

N2 - During embryogenesis, chordates pass through a tailbud stage in which the larval tail is formed. Since acquisition of a tadpole-like tail during tailbud stage is one of the key events in the evolution of chordates, understanding the anatomy of the tailbud stage chordate embryo is of special interest. In this study, to understand comprehensively the anatomy of the tailbud embryo at single-cell-level, real microscopic image stacks of the tailbud embryo in Ciona intestinalis were reconstructed into a 3D computer model. This comprehensive 3D model of the ascidian tailbud embryo was based on real images of confocal laser scanning microscope (CLSM) and therefore, cell shape, location and cell arrangement reflect real geometries of the tailbud embryo. We found that the tailbud embryo consists of 1579 cells, including 836 epidermal cells, 228 cells in the central nervous system, 218 mesenchymal cells, four trunk ventral cells, two B/B*8.11 cells, 36 muscle cells, 40 notochord cells, four primordial germ cells, and 199 endodermal cells. Moreover, we identified for the first time two populations of previously undefined cells (a total of 12 cells) in Ciona: one located in the lateral trunk and the other located under the tail dorsal epidermis. This information provides a first step for understanding how the body plan of the chordate tailbud embryo formed and evolved.

AB - During embryogenesis, chordates pass through a tailbud stage in which the larval tail is formed. Since acquisition of a tadpole-like tail during tailbud stage is one of the key events in the evolution of chordates, understanding the anatomy of the tailbud stage chordate embryo is of special interest. In this study, to understand comprehensively the anatomy of the tailbud embryo at single-cell-level, real microscopic image stacks of the tailbud embryo in Ciona intestinalis were reconstructed into a 3D computer model. This comprehensive 3D model of the ascidian tailbud embryo was based on real images of confocal laser scanning microscope (CLSM) and therefore, cell shape, location and cell arrangement reflect real geometries of the tailbud embryo. We found that the tailbud embryo consists of 1579 cells, including 836 epidermal cells, 228 cells in the central nervous system, 218 mesenchymal cells, four trunk ventral cells, two B/B*8.11 cells, 36 muscle cells, 40 notochord cells, four primordial germ cells, and 199 endodermal cells. Moreover, we identified for the first time two populations of previously undefined cells (a total of 12 cells) in Ciona: one located in the lateral trunk and the other located under the tail dorsal epidermis. This information provides a first step for understanding how the body plan of the chordate tailbud embryo formed and evolved.

KW - Anatomy

KW - Cell lineage

KW - Chordate evolution

KW - Interactive 3D visualization

KW - Segmentation

KW - Tunicate

UR - http://www.scopus.com/inward/record.url?scp=84868300161&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84868300161&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2012.09.007

DO - 10.1016/j.ydbio.2012.09.007

M3 - Article

C2 - 23022659

AN - SCOPUS:84868300161

VL - 372

SP - 274

EP - 284

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -