Three dimensional closed microchamber for evaluation of shapes effect on stem cell differentiation

Y. Matsushiro, M. Kato-Negishi, Hiroaki Onoe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We report polyethylene glycol diacrylate (PEGDA) three-dimensional (3D) closed microchambers for assaying 3D-shape-driven differentiation induction of stem cells. We succeeded in fabricating PEGDA microchambers of various 3D shapes. We then cultured mouse neural stem cells (mNSCs) in the microchambers that was closed with by an agarose cover. We confirmed the high sealability of the closed microchambers and that the shapes of cultured mNSCs followed the 3D shape of the microchambers. We believe that our PEGDA microchambers would control 3D shapes of tissues and be useful for examining 3D-shape-driven differentiation induction of stem cells.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages449-450
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16/10/916/10/13

Fingerprint

Stem cells
Polyethylene glycols
Tissue

Keywords

  • Differentiation induction
  • Micropatterning
  • PEGDA hydrogel
  • Stem cell

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Matsushiro, Y., Kato-Negishi, M., & Onoe, H. (2016). Three dimensional closed microchamber for evaluation of shapes effect on stem cell differentiation. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 449-450). Chemical and Biological Microsystems Society.

Three dimensional closed microchamber for evaluation of shapes effect on stem cell differentiation. / Matsushiro, Y.; Kato-Negishi, M.; Onoe, Hiroaki.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 449-450.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Matsushiro, Y, Kato-Negishi, M & Onoe, H 2016, Three dimensional closed microchamber for evaluation of shapes effect on stem cell differentiation. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, pp. 449-450, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 16/10/9.
Matsushiro Y, Kato-Negishi M, Onoe H. Three dimensional closed microchamber for evaluation of shapes effect on stem cell differentiation. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 449-450
Matsushiro, Y. ; Kato-Negishi, M. ; Onoe, Hiroaki. / Three dimensional closed microchamber for evaluation of shapes effect on stem cell differentiation. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 449-450
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AB - We report polyethylene glycol diacrylate (PEGDA) three-dimensional (3D) closed microchambers for assaying 3D-shape-driven differentiation induction of stem cells. We succeeded in fabricating PEGDA microchambers of various 3D shapes. We then cultured mouse neural stem cells (mNSCs) in the microchambers that was closed with by an agarose cover. We confirmed the high sealability of the closed microchambers and that the shapes of cultured mNSCs followed the 3D shape of the microchambers. We believe that our PEGDA microchambers would control 3D shapes of tissues and be useful for examining 3D-shape-driven differentiation induction of stem cells.

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