Three-dimensional culture using human plasma-medium gel with fragmin/protamine microparticles for proliferation of various human cells

Satoko Kishimoto, Masayuki Ishihara, Makoto Takikawa, Megumi Takikawa, Yuki Sumi, Shingo Nakamura, Masanori Fujita, Toshinori Sato, Tomoharu Kiyosawa

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Fragmin/protamine microparticles (F/P MPs) have been used as carriers for the preservation and activation of cytokines in human plasma (HP)–Dulbecco’s modified Eagle’s medium (DMEM) gels. This study investigated a three-dimensional (3D) culture system using an HP–DMEM gel with 0.1 mg/mL F/P MPs and 5 ng/mL FGF-2 for the proliferation of human dermal fibroblast cells (DFCs), human microvascular endothelial cells (MVECs) and human coronary smooth muscle cells (SMCs), or 5 ng/mL interleukin (IL)-3/granulocyte-macrophage colony-stimulating factor (GM-CSF) for a human hematopoietic cell line (TF-1 cells). DFCs, MVECs, SMCs and TF-1 cells grew rapidly under 3D culture conditions using a low-concentration HP (2 %)–DMEM gel with F/P MPs and FGF-2 (for DFCs, MVECs and SMCs) or IL-3/GM-CSF (for TF-1 cells) at doubling times of 22, 23, 25 and 18 h, respectively, without the use of animal serum, compared to under 2D culture conditions using low-concentration human serum (2 %)–DMEM with 5 ng/mL FGF-2 or IL-3/GM-CSF on F/P MP-coated plates at doubling times of approximately 26, 25, 40 and 20 h, respectively.

Original languageEnglish
Pages (from-to)791-802
Number of pages12
JournalCytotechnology
Volume66
Issue number5
DOIs
Publication statusPublished - 2014 Oct 1

Keywords

  • 3D culture
  • Cytokine carrier
  • Fragmin/protamine microparticles (F/P MPs)
  • Plasma–medium gel

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

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