Three dimensional spheroids formation platform using micro-rotation flow

Hiroki Ota, Koji Deguchi, Shiny Kita, Ryosuke Yamamoto, Norihisa Miki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Hepatocytes are responsible for metabolic and detoxification processes in the liver. During the tissue formation from isolated cells, they increase the cell density and acquire their liver-specific functions, new protein expression and cell signaling. Hence, tissue-based in vitro studies are strongly demanded. We develop a MEMS platform which forms 3D spheroids by microfluodic handing of cells. The microfludic platform is composed of PDMS chamber fabricated by microlithography and a circulation system. We simulated the flow in the chamber by FLUENT and found that micro-rotation flow was generated when the chamber height was four times greater than the channel height. The rotation flow was experimentally observed using fluorescent microbeads with the newly designed micro-chamber. The proposed MEMS-based 3D-spheroids formation platform is readily to create a technological innovation in biology as a bridge between cell researches and tissue researches.

Original languageEnglish
Title of host publication2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
Pages202-207
Number of pages6
DOIs
Publication statusPublished - 2008 Dec 1
Event2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation" - Nagoya, Japan
Duration: 2008 Nov 62008 Nov 9

Publication series

Name2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008

Other

Other2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
CountryJapan
CityNagoya
Period08/11/608/11/9

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Human-Computer Interaction
  • Electrical and Electronic Engineering

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