Three types of macrophagic cells in the mesentery of mice with special reference to LYVE-1-immunoreactive cells

Miao Zheng, Shunsuke Kimura, Junko Nio-Kobayashi, Hiromi Takahashi-Iwanaga, Toshihiko Iwanaga

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Immunohistochemistry using whole mount preparations of the murine mesentery revealed two types of LYVE-1-immunoreactive cells with dendritic morphology other than F4/80+ typical macrophages. The two types of LYVE-1+ cells were regularly distributed with constant intervals throughout the mesentery and appeared to possess their own territory. Both types of LYVE-1+ cells were weakly or moderately immunopositive for F4/80 antibody, a marker of macrophages, while F4/80+ round macrophages were absolutely free from the LYVE-1 immunoreactivity. Only macrophages could ingest latex particles of 20 nm in diameter 3 h after a peritoneal injection. Peritoneal administration of lipopolysaccharide (LPS) induced a rapid reduction of LYVE-1 immunoreactivity in the cells with dendritic morphology followed by an increased immunoreactivity to F4/80 antibody, and simultaneously by dynamic changes in their shape. Under normal conditions, F4/80+ macrophages in various connective tissues expressed LYVE-1, in contrast to lack of LYVE-1 in F4/80+ macrophages within the parenchyma of visceral organs and macrophages residing in hepatic sinusoids and pulmonary alveoli. LYVE-1 may play a role in cell adhesion and migration of macrophagic cells within connective tissues rich in hyaluronan, and loss of LYVE-1 becomes a reliable sign of activated conditions in inflammation.

Original languageEnglish
Pages (from-to)37-45
Number of pages9
JournalBiomedical Research (Japan)
Volume35
Issue number1
DOIs
Publication statusPublished - 2014 Mar 4
Externally publishedYes

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Mesentery
Macrophages
Dendritic Cells
Pulmonary Alveoli
Tissue
Connective Tissue Cells
Antibodies
Cell adhesion
Latex
Hyaluronic Acid
Microspheres
Cell Adhesion
Connective Tissue
Cell Movement
Lipopolysaccharides
Immunohistochemistry
Cells
Inflammation
Injections
Liver

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Three types of macrophagic cells in the mesentery of mice with special reference to LYVE-1-immunoreactive cells. / Zheng, Miao; Kimura, Shunsuke; Nio-Kobayashi, Junko; Takahashi-Iwanaga, Hiromi; Iwanaga, Toshihiko.

In: Biomedical Research (Japan), Vol. 35, No. 1, 04.03.2014, p. 37-45.

Research output: Contribution to journalArticle

Zheng, Miao ; Kimura, Shunsuke ; Nio-Kobayashi, Junko ; Takahashi-Iwanaga, Hiromi ; Iwanaga, Toshihiko. / Three types of macrophagic cells in the mesentery of mice with special reference to LYVE-1-immunoreactive cells. In: Biomedical Research (Japan). 2014 ; Vol. 35, No. 1. pp. 37-45.
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